Recombinant Mouse Interleukin-36 Receptor Antagonist Protein (IL36RN) Protein (His/Tag-Free)

Name: Recombinant Mouse Interleukin-36 Receptor Antagonist Protein (IL36RN) Protein (His/Tag-Free)
Brand: Beta LifeScience
SKU: BLC-05308P
Availability: InStock

Collections: Cytokines, Cytokines and growth factors, Interleukins and receptors, Recombinant proteins

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Product Overview

Description	The Recombinant Mouse Interleukin-36 Receptor Antagonist Protein Protein is produced by our E.coli expression system.
Purity	Greater than 85% as determined by SDS-PAGE and HPLC.
Uniprotkb	Q9QYY1
Target Symbol	IL36RN
Synonyms	IL36RN; Fil1d; Il1f5; Il1h3; Il1hy1; Interleukin-36 receptor antagonist protein; IL-36Ra; Interleukin-1 HY1; IL-1HY1; Interleukin-1 delta; IL-1 delta; Interleukin-1 family member 5; IL-1F5; Interleukin-1 homolog 3; IL-1H3; Interleukin-1-like protein 1; IL-1L1
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-His/Tag-Free
Complete Sequence	VLSGALCFRM KDSALKVLYL HNNQLLAGGL HAEKVIKGEE ISVVPNRALD ASLSPVILGV QGGSQCLSCG TEKGPILKLE PVNIMELYLG AKESKSFTFY RRDMGLTSSF ESAAYPGWFL CTSPEADQPV RLTQIPEDPA WDAPITDFYF QQCD
Mol. Weight	16.9 kDa
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.

Target Details

Target Function	Inhibits the activity of interleukin-36 (IL36A,IL36B and IL36G) by binding to receptor IL1RL2/IL-36R and preventing its association with the coreceptor IL1RAP for signaling. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor. Proposed to play a role in skin inflammation. May be involved in the innate immune response to fungal pathogens. May activate an anti-inflammatory signaling pathway by recruiting SIGIRR.
Subcellular Location	Cytoplasm. Secreted.
Protein Families	IL-1 family
Database References	KEGG: mmu:54450 STRING: 10090.ENSMUSP00000028360 UniGene: Mm.29261
Tissue Specificity	Highly abundant in embryonic tissue and tissues containing epithelial cells.

Gene Functions References

Transcripts for IL-1F5 are significantly increased in the involved skin of bi- and monotransgenic mice compared with their C57BL controls. PMID: 21242515
IL-1F5 has unique loop conformations for receptor binding specificity PMID: 12974628
IL-1F5 mediates anti-inflammatory effects through its ability to induce interleukin-4 production, as a consequence of its interaction with orphan receptor single Ig IL-1R-related molecule (SIGIRR)/TIR8. PMID: 18284608

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.