Recombinant Mouse Interleukin-7 Receptor Subunit Alpha (IL7R) Protein (His)

Name: Recombinant Mouse Interleukin-7 Receptor Subunit Alpha (IL7R) Protein (His)
Brand: Beta LifeScience
SKU: BLC-09291P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: Cytokines, Cytokines and growth factors, Interleukins and receptors, Recombinant proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Submit an inquiry today to inquire about all available size options and prices! Connect with us via the live chat in the bottom corner to receive immediate assistance.

Product Overview

Description	Recombinant Mouse Interleukin-7 Receptor Subunit Alpha (IL7R) Protein (His) is produced by our Baculovirus expression system. This is a extracellular protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	P16872
Target Symbol	IL7R
Synonyms	Il7r; Interleukin-7 receptor subunit alpha; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; CD antigen CD127
Species	Mus musculus (Mouse)
Expression System	Baculovirus
Tag	C-9His
Target Protein Sequence	ESGNAQDGDLEDADADDHSFWCHSQLEVDGSQHLLTCAFNDSDINTANLEFQICGALLRVKCLTLNKLQDIYFIKTSEFLLIGSSNICVKLGQKNLTCKNMAINTIVKAEAPSDLKVVYRKEANDFLVTFNAPHLKKKYLKKVKHDVAYRPARGESNWTHVSLFHTRTTIPQRKLRPKAMYEIKVRSIPHNDYFKGFWSEWSPSSTFETPEPKNQGGWD
Expression Range	21-239aa
Protein Length	Extracellular Domain
Mol. Weight	27.0 kDa
Research Area	Immunology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).
Subcellular Location	Membrane; Single-pass type I membrane protein.
Protein Families	Type I cytokine receptor family, Type 4 subfamily
Database References	KEGG: mmu:16197 STRING: 10090.ENSMUSP00000003981 UniGene: Mm.389
Tissue Specificity	Spleen, thymus and fetal liver.

Gene Functions References

Memory formation and long-term maintenance of memory type I innate lymphoid cells via a lymph node-liver axis is mediated via IL-7Ralpha expression. PMID: 30451860
IL-7 receptor (IL-7R) is not strictly required for the development of any innate lymphoid cells subset. PMID: 28361874
the results indicate that endogenous IL-7R signals promote Th1 and Tc1 responses and IFN-gamma- and TNF-alpha production to sustain the persistence of SS-like sialadenitis in NOD mice. These findings suggest that IL-7 and Th1 cytokines could serve as promising therapeutic targets for this prevalent autoimmune disease. PMID: 29680668
this study demonstrates a key role for IL-7R in the generation of microenvironments required for thymic dendritic cells PMID: 28890536
show that the differentiation of group 3 ILCs is controlled by the glutamylation of IL-7Ralpha and the induction of transcription factor Sall3 PMID: 28794449
results demonstrated that forced expression of IL-7R not only improved the functionality of tolerized CD8 T cells, it also acted in synergy with PDL-1 deficiency to further promote CD8 T cell cytotoxicity to self antigens PMID: 29272267
this study shows that short-term blockade of IL-7Ralpha induces detectable changes in co-inhibitory receptor expression and Treg frequencies in peripheral blood of NOD mice PMID: 28356069
results indicate that the induction of IL-7Ralpha expression on dendritic cells (DCs) is critical for thymic stromal lymphopoietin responsiveness and that IL-4 can upregulate IL-7Ralpha on DCs. PMID: 28404633
IL-7RA role in hematopoiesis and development of the lympho-myeloid progenitors in the developing fetal liver PMID: 26235516
These studies provide in vivo evidence that IL-7Ralpha signals positively regulate normal human B-cell production and proliferation beyond the fetal period and suggest that TSLP can replace IL-7 in providing these signals. PMID: 27325567
Bcl6 promoted T follicular helper cell differentiation through antagonizing IL-7R / STAT5a axis. PMID: 26876184
results suggest that LNK suppresses IL-7R/JAK/STAT signaling to restrict pro-/pre-B progenitor expansion and leukemia development, providing a pathogenic mechanism and a potential therapeutic approach for B-ALLs with LNK mutations. PMID: 26974155
The IL-7R plays an important role in the induction of HFD-induced adipogenesis and insulin resistance in mice. PMID: 26152662
IL-7R signaling in regulatory T cells maintains peripheral and allograft tolerance in mice. PMID: 26450881
This work demonstrates that the CNS1 element controls IL-7Ralpha expression and maintenance of peripheral T cells, suggesting differential regulation of IL-7Ralpha expression between central and peripheral lymphoid organs. PMID: 26336149
Inhibition of the IL-7 receptor inhibits tumor growth in murine models of melanoma. PMID: 24619454
The combined loss of Gads and CD127 reveals a novel function of Gads prior to T cell receptor beta expression PMID: 25037454
data suggest that IL-7Ralpha Tyr(449) was essential for IL-7Ralpha signaling in bone marrow B cell development and survival. PMID: 25143441
The development and survival but not function of follicular B cells is dependent on IL-7Ralpha Tyr449 signaling. PMID: 24551160
interleukin-7 receptor mutants induce early T cell precursor acute lymphoblastic leukemia by the block of thymocyte differentiation at the double negative 2 stage at which myeloid lineage and T lymphocyte developmental potential coexist. PMID: 24687960
The transcription factor GATA3 is critical for the development of all IL-7Ralpha-expressing innate lymphoid cells. PMID: 24631153
Expression of insertion mutation in combination with a Notch1 mutant led to the development of much more aggressive T-acute lymphoblastic leukemia than with wild-type IL7Ralpha. PMID: 24174626
These data indicate that IL7R+ B cells have a proinflammatory role in arthritis which can be inhibited by the sympathetic neurotransmitter norepinephrine via inhibition of IL-7R signalling. PMID: 23505234
IL-7Ralpha transgenesis or T-cell-specific ablation of Gfi-1 restored IL-7Ralpha expression and largely ameliorated the development and homeostasis defects of TGF-beta-receptor-deficient T cells. PMID: 23932572
We have analyzed the discrete contributions of the antibody constant (Fc) and IL-7-binding (Fab) domains to the mechanism of binding to IL-7R. PMID: 23610371
IL7Ralpha has a role in potentiating IL-7 bioactivity and in promoting autoimmunity PMID: 23610432
Pre-B cell receptor (BCR) cooperates with IL-7R in expanding the pre-B cell pool, but it also critically controls the differentiation program that shuts off the c-Myc gene in large pre-B cells. PMID: 23420891
IL-7Ralpha differentially controls development and maturation of thymocyte subpopulations in late developmental stages. PMID: 23267098
the in vivo regulatory role of Gfi1 was specific for CD8(+), and not CD4(+) T cells or immature thymocytes. These results identify Gfi1 as a specific transcriptional repressor of the Il7r gene in CD8 T lymphocytes in vivo. PMID: 22865857
show that interleukin-17A (IL-17A)-producing IL-7Ralpha(+) innate lymphoid cells (ILCs) were potent promoters of disease in Tbx21(-/-)Rag2(-/-) ulcerative colitis (TRUC) mice PMID: 23063332
The durable efficacy and the multipronged tolerogenic mechanisms of IL-7Ralpha antibody therapy suggest a unique disease-modifying approach to T1D. PMID: 22733769
Blockade of IL-7/IL-7Ralpha signaling in IL-7-dependent p53(null) cells reduced POT1 expression and caused telomere and chromosome abnormalities similar to those observed in DKO lymphomas. PMID: 22281704
Data suggest that this decrease in IL-7Ralpha is associated with defective hematopoietic development in Down Syndrome. PMID: 21504363
Increased IL-7Ralpha (CD127) expression correlated, as previously reported with increased turnover of CD4 memory cells, however, this was not linked to protection or enhanced response to rechallenge PMID: 22039531
a novel function of Efnb1 and Efnb2 in stabilizing IL-7Ralpha expression at the post-translational level PMID: 22069310
IL-7R-mediated signaling not only maintains the size but also impacts the diversity of the naive T-cell repertoire. PMID: 21339767
IL-7R-dependent survival and differentiation of early T-lineage progenitors is regulated by the BTB/POZ domain transcription factor Miz-1. PMID: 21258009
High expression of IL-7 receptor (IL-R)alpha on colitic CD4-positive memory T cells, but not on other cells, is essential for the development and persistence of chronic colitis. PMID: 21217010
These findings highlight that IL-7Ralpha confers susceptibility by influencing autoimmune Th1/Th17 responses in experimental autoimmune encephalomyelitis model of multiple sclerosis . PMID: 20861865
T-cell homeostasis is maintained through a concerted regulation of Gfi-1-IL-7R-controlled cytokine responsiveness and ERK-mediated TCR signaling strength by the Cdc42-PAK1 signaling axis. PMID: 20937872
increased expression on activated FOXP3+CD4+ regulatory T cells PMID: 20690182
HIV Tat protein colocalizes with CD127 at the cell surface of resting CD8-positive T lymphocytes. PMID: 20660706
Sin1-mTORC2 suppresses rag1,2 and il7r gene expression through Akt2 in B cells. PMID: 20705244
study shows that IL-7Ralpha Y449 is important for lymphocyte transformation PMID: 20440272
Pre-pro-B cell growth-stimulating factor (PPBSF) upregulates IL-7Ralpha chain expression and enables pro-B cells to respond to monomeric IL-7. PMID: 12184921
IL-7R(alpha)(-/-) mice lack detectable numbers of all downstream early B lineage precursors, including pre-pro-B cells PMID: 12208884
IL-7R signals are able to stimulate T cell proliferation in lymphopenic hosts independently of lck-mediated T cell receptor (TCR) signaling, but they can also synergize with TCR signals to facilitate proliferation. PMID: 12244169
IL-2 signaling can diminish IL-7Ralpha expression via a phosphatidylinositol 3-kinase/Akt-dependent mechanism. PMID: 12354940
The inability of B-cell progenitors in the thymus to mature is due to down-regulation of IL7Ralpha through a type 1 IFN receptor-independent mechanism PMID: 12393710
Bax deficiency partially corrects interleukin-7 receptor alpha deficiency PMID: 12433363

FAQs

Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.

Feel free to use the Chat function to initiate a live chat. Our customer representative can provide you with a quote immediately.

Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.