Recombinant Mouse Interleukin-9 Protein (IL9), Active

Name: Recombinant Mouse Interleukin-9 Protein (IL9), Active
Brand: Beta LifeScience
SKU: BLC-05362P
Availability: InStock

Collections: Antibody / cell therapy targets, Antibody therapeutic / adc target proteins, Cytokines, Cytokines and growth factors, Interleukins and receptors, Recombinant proteins

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Product Overview

Description	Recombinant Mouse Interleukin-9 Protein (IL9), Active is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 97% as determined by SDS-PAGE and HPLC.
Endotoxin	Less than 1.0 EU/μg as determined by LAL method.
Activity	Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine TS1 cells is less than 0.02 ng/ml, corresponding to a specific activity of >5.0x10 7 IU/mg.
Uniprotkb	P15247
Target Symbol	IL9
Synonyms	Il9; Interleukin-9; IL-9; Cytokine P40; T-cell growth factor P40
Species	Mus musculus (Mouse)
Expression System	E.Coli
Tag	Tag-Free
Complete Sequence	QRCSTTWGIR DTNYLIENLK DDPPSKCSCS GNVTSCLCLS VPTDDCTTPC YREGLLQLTN ATQKSRLLPV FHRVKRIVEV LKNITCPSFS CEKPCNQTMA GNTLSFLKSL LGTFQKTEMQ RQKSRP
Expression Range	19-144aa
Protein Length	Full Length of Mature Protein
Mol. Weight	14.2 kDa
Research Area	Immunology
Form	Liquid or Lyophilized powder
Buffer	0.2 μm filtered PBS, pH 7.4, lyophilized
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.

Target Details

Target Function	Supports IL-2 independent and IL-4 independent growth of helper T-cells.
Subcellular Location	Secreted.
Protein Families	IL-7/IL-9 family
Database References	KEGG: mmu:16198 STRING: 10090.ENSMUSP00000022019 UniGene: Mm.3006

Gene Functions References

Results indicated that interleukin 9 (IL-9) was an important type of cytokine involved in the progression of Schistosoma japonicum infection-induced hepatic damage. PMID: 28539607
IL9 CNS-25/IL9 CNS-18 is a critical and conserved regulatory element for IL-9 production in mice and humans. PMID: 30442929
Data show that interferon regulatory factor 1 (IRF1) occupancy correlates with decreased interferon regulatory factor 4 (IRF4) abundance, suggesting an IRF1-IRF4-binding competition at the interleukin 9 (Il9) locus. PMID: 28497800
Furthermore, chromatin immunoprecipitation (ChIP) followed by luciferase assays revealed direct binding of Foxo1 to both the Il9 and Irf4 promoters and induces their transactivation. PMID: 29339772
CXCL10, not CXCL9 or CXCL11, induced IL-9 expression in the liver tissue. PMID: 29860220
Chronic kidney disease aggravates vein graft disease through mechanisms involving IL-9 and mast cell activation. PMID: 29048463
the results of this study indicate that environmental cues dictate the instability of the Th9 phenotype, and they suggest approaches to enhance Th9 activity in beneficial immune responses PMID: 28137893
The germinal center development of memory B cells is promoted by follicular helpter T cell-derived IL-9. PMID: 28650481
In mice, the absence of IL-9 impaired ILC2 proliferation and activation of regulatory T (Treg) cells, and resulted in chronic arthritis with excessive cartilage destruction and bone loss. PMID: 28714991
These data indicate that IL-9 is an essential regulator of megakaryopoiesis and a promising therapeutic agent for treatment of thrombocytopenia such as CIT. PMID: 28450306
Group 2 innate lymphoid cells utilize the IRF4-IL-9 module to coordinate epithelial cell maintenance of lung homeostasis. PMID: 26129648
Our results suggest that The Th9/IL-9 is involved in the pathogenesis of UC. PMID: 26603936
IL-9 exerted pro-atherosclerotic effects in ApoE-/- mice at least partially by inducing VCAM-1 expression, which mediated inflammatory cell infiltration into atherosclerotic lesions. PMID: 25784693
IL-9 is dispensable for mucosal mast cell development but is necessary for their effective expansion to promote intestinal mastocytosis and susceptibility to experimental food allergy in an IL-9-dependent autocrine manner. PMID: 26410628
IL-9 production following chlamydial lung infection is redundant for host defense against the intracellular bacteria PMID: 25646821
IL-25-stimulated dendritic cells rapidly induced mediators, such as the chemokine CCL17, which, in turn, attracted IL-9-producing T cells PMID: 26371249
TH9 cells are a major source of IL-9 in models of allergic inflammation and play an important role in mast cell accumulation and activation. PMID: 25746972
IRF4 binds to the promoters of IL1b and IL9, suggesting that tick-derived sialostatin L suppresses mast cell-derived IL-9 preferentially by inhibiting IRF4. PMID: 26078269
these data identify TL1A-DR3 interactions as a novel pathway that promotes Th9 differentiation and pathogenicity. TL1A may be a potential therapeutic target in diseases dependent on IL-9. PMID: 25786692
Neutralizing IL9 antibody phenocopied the effects of IL9 gene deletion. PMID: 25297635
IL-9 can promote interstitial cells of Cajal (ICC) growth and help maintain ICC functions. PMID: 24755995
Th2 lymphocytes migrating to the bone marrow under high-altitude hypoxia promote erythropoiesis via activin A and interleukin-9. PMID: 24769210
results suggest that Foxp3 Treg suppress sufficient IL-9 production for subsequent mast cell degranulation during S. ratti infection in a non-redundant manner in BALB/c mice PMID: 24516385
This study demonstrates that IL-9, through its direct effects on Th1 and ability to promote IL-4 secretion, has a regulatory role for Th1 lymphocytes in allergic contact dermatitis. PMID: 24487305
In experimental autoimmune encephalomyelitis, forced expression of BCL6 in myelin oligodendrocyte glycoprotein35-55-specific Th9 cells resulted in decreased IL-9 production and induction of IFN-gamma PMID: 24879792
The activator protein 1 (AP1) family transcription factor BATF (B cell, activating transcription factor-like) was among the genes enriched in Th9 cells and was required for the expression of IL-9 and other Th9-associated genes. PMID: 24216482
IL-9 has a unique role as an autocrine amplifier of ILC2 function, promoting tissue repair in the recovery phase after helminth-induced lung inflammation PMID: 24249111
TGF-beta signaling regulated IL-9 production through displacement of EZH2 and removal of suppressive H3K27 histone modification at the Il9 locus. PMID: 24108699
There is a critical and nonredundant role for Th9 cells and IL-9 in host-protective type 2 immunity against parasitic worm infection. PMID: 24138883
This study demonstrates that intestinal IL-25 promotes protective immunity against T. spiralis infection by inducing antigen-specific Th9 immune response. PMID: 23897610
We propose that Smad2/3 and IRF4 cooperatively transactivate the Il9 promoter and play an important role in regulating allergic immune responses by inducing Th9 cells. PMID: 23913959
Il9r(-/-) mice showed accelerated tumor growth. Recombinant IL-9 given to tumor-bearing WT & Rag1(-/-) mice inhibited melanoma & lung carcinoma growth, suggesting a role for IL9 in tumor immunity. PMID: 22772464
IL-9-producing invariant NKT cells protect against DSS-induced colitis in an IL-4-dependent manner. PMID: 22892939
A PU.1-dependent mechanism alters histone acetylation and expression of the interleukin (Il)9 locus in T helper (Th)9 cells. PMID: 22904310
propose that Notch and Smad3 cooperate to induce IL-9 and participate in regulating the immune response PMID: 22503540
data established a transcription factor network that regulates IL-9 PMID: 22180613
data suggest that sialostatin L can prevent experimental asthma, most likely by inhibiting the IL-9 production of Th9 cells PMID: 22327077
production is largely restricted to innate lymphoid cells in experimental lung inflammation PMID: 21983833
Data show that IL-9(-/-) mice developed significantly less severe EAE than their WT counterparts. PMID: 21674475
data show that neoplastic mast cells (MC) express IL-9 receptors, that IL-9 and NPM-ALK upregulate MC-production in vivo, and that both'hits' act in concert to induce a mastocytosis-like disease in mice PMID: 21297223
IL-9-producing CD4-positive T cells are induced in an IL-4-dependent manner. PMID: 21346235
results demonstrate a previously unappreciated role for IL-9 in dampening the pathogenic activities of Th17 cells PMID: 21360526
The nephroprotective, anti-inflammatory effects of regulatory T cells critically depend on IL-9-mediated attraction of mast cells into kidney-draining lymph nodes. PMID: 21115728
protective effect of IL-9 blockade in EAE was likely mediated via inhibition of the development of MOG peptide-specific T cells, which in turn led to reduced infiltration of T cells into the CNS PMID: 20805418
data clearly demonstrate the central function of interferon regulatory factor 4 in the development of T helper 9 cells and underline the contribution of this T helper cell subset to the pathogenesis of asthma. PMID: 20674401
the chronic OVA challenge protocol in BALB/c mice provides a model to study the mechanism of bronchial mast cell accumulation, and bronchial mast cell accumulation in chronic OVA challenged mice is independent of IL-9 in this model PMID: 20480160
a critical role for PU.1 in generating the IL-9-producing (T(H)9) phenotype and in the development of allergic inflammation PMID: 20431622
These studies indicate differential involvement of the IL-9/IL-9R pathway in systemic and oral antigen-induced anaphylaxis. PMID: 20159257
IL-25 is a newly identified regulator of IL-9 expression. PMID: 20154671
expression in anaplastic large-cell lymphoma PMID: 12555065

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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