Recombinant Mouse Nicotinamide Phosphoribosyltransferase (NAMPT) Protein (His)
Beta LifeScience
SKU/CAT #: BLC-01459P
Greater than 85% as determined by SDS-PAGE.
Recombinant Mouse Nicotinamide Phosphoribosyltransferase (NAMPT) Protein (His)
Beta LifeScience
SKU/CAT #: BLC-01459P
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Description | Recombinant Mouse Nicotinamide Phosphoribosyltransferase (NAMPT) Protein (His) is produced by our Yeast expression system. This is a full length protein. |
Purity | Greater than 85% as determined by SDS-PAGE. |
Uniprotkb | Q99KQ4 |
Target Symbol | NAMPT |
Synonyms | Pre-B-cell colony-enhancing factor 1 homolog Short name: PBEF Visfatin |
Species | Mus musculus (Mouse) |
Expression System | Yeast |
Tag | N-10His |
Target Protein Sequence | MNAAAEAEFNILLATDSYKVTHYKQYPPNTSKVYSYFECREKKTENSKVRKVKYEETVFYGLQYILNKYLKGKVVTKEKIQEAKEVYREHFQDDVFNERGWNYILEKYDGHLPIEVKAVPEGSVIPRGNVLFTVENTDPECYWLTNWIETILVQSWYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYRGVSSQETAGIGASAHLVNFKGTDTVAGIALIKKYYGTKDPVPGYSVPAAEHSTITAWGKDHEKDAFEHIVTQFSSVPVSVVSDSYDIYNACEKIWGEDLRHLIVSRSTEAPLIIRPDSGNPLDTVLKVLDILGKKFPVTENSKGYKLLPPYLRVIQGDGVDINTLQEIVEGMKQKKWSIENVSFGSGGALLQKLTRDLLNCSFKCSYVVTNGLGVNVFKDPVADPNKRSKKGRLSLHRTPAGNFVTLEEGKGDLEEYGHDLLHTVFKNGKVTKSYSFDEVRKNAQLNIEQDVAPH |
Expression Range | 1-491aa |
Protein Length | Full Length |
Mol. Weight | 57.9 kDa |
Research Area | Cytokine |
Form | Liquid or Lyophilized powder |
Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
Storage | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Target Details
Target Function | The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression. |
Subcellular Location | Nucleus. Cytoplasm. Secreted. |
Protein Families | NAPRTase family |
Database References | |
Tissue Specificity | Ubiquitously expressed in lymphoid and non-lymphoid tissues. |
Gene Functions References
- our study demonstrated that PBEF ameliorates ischemia-induced neuronal death through inhibiting caspase-dependent and independent apoptotic signaling pathways and suppressing mitochondrial damage and dysfunction. Our study provides novel insights into the mechanisms underlying the neuroprotective effect of PBEF, and helps to identify potential targets for ischemic stroke therapy. PMID: 27576732
- the NAMPT-mediated NAD(+) biosynthesis pathway is a potential therapeutic target for degenerative motor neuron diseases. PMID: 28854367
- observations demonstrate that NAMPT knockdown exerted antiatherogenic effects by promoting cholesterol efflux and macrophage RCT through the PPARalpha- LXRalpha- ABCA1/G1pathway in vitro and in vivo PMID: 27229177
- Study found that primary mouse embryonic fibroblast (MEF) cells undergo progressive decline of NAMPT and NAD(+) contents during serial passaging before becoming senescent; further found that constitutive Nampt over-expression increases SIRT1 activity, increases the expression of antioxidant genes, superoxide dismutase 2 and catalase and promotes resistance against oxidative stress. PMID: 29178516
- NAMPT-mediated NAD(+) biosynthesis in adipocytes regulates adipose tissue function and multi-organ insulin sensitivity in mice. PMID: 27498863
- NAMPT-mediated NAD(+) biosynthesis is essential for vision in mice. PMID: 27681422
- NAMPT is involved in key basic cellular functions. PMID: 28333140
- role of Nampt in regulating osteoblast differentiation in primary mouse bone marrow-derived mesenchymal stem cells (BM-MSCs); Nampt expression was progressively elevated during BM-MSCs osteogenic differentiation PMID: 28159473
- Synthesis and Degradation of Adenosine 5'-Tetraphosphate by Nicotinamide and Nicotinate Phosphoribosyltransferases PMID: 28416276
- NAMPT inhibited endothelial progenitor cell senescence through an SIRT1 AS lncRNA/miR-22/SIRT1 pathway PMID: 27569277
- results support a novel, important role for Nampt-mediated NAD(+) biosynthesis in long-term depression and in the function of GluN2B-containing NMDARs PMID: 26481044
- extracellular NAMPT can be actively released by melanoma cells in vitro. PMID: 26358657
- Visfatin plays an important role in glucose metabolism via the calcium mediated AMPK-p38 MAPK pathway. PMID: 26019302
- Deacetylation of intracellular NAMPT by the mammalian NAD(+)-dependent deacetylase SIRT1 predisposes the protein to secretion in adipocytes. PMID: 25921090
- Report retinal toxicity of NAMPT inhibitors. PMID: 25505128
- intracellular NAMPT-NAD(+)-SIRT1 cascade improves post-ischaemic neovascularization PMID: 25341895
- The Nampt activity of visfatin is involved in chondrocyte and osteoblast activation PMID: 24479481
- Visfatin levels increase after aortic valve replacement for aortic stenosis and may play a part in postoperative reverse remodelling. PMID: 24429804
- nicotinamide phosphoribosyltransferase increases NAD synthesis in muscle but is not sufficient to promote oxidative metabolism PMID: 25411251
- NAMPT is a key novel ARDS therapeutic target and candidate gene with genetic/epigenetic transcriptional regulation in response to excessive mechanical stress. PMID: 24821571
- Ischemic Injury Induces Nicotinamide Phosphoribosyltransferase Expression and Secretion from Neurons. PMID: 25005877
- Pharmacological inhibition of NAMPT activity mitigates inflammation in atherosclerotic plaques by reducing CXCL1-mediated activities on neutrophils. PMID: 24196571
- Oral lipid ingestion is a physiological regulator of systemic visfatin release. Fatty acids differentially regulate visfatin release in vitro. PMID: 24554513
- Fndings support NAMPT inhibition via FK-866 as a novel therapeutic agent for acute respiratory distress syndrome via enhanced apoptosis in inflammatory neutrophils. PMID: 24588101
- results indicate that JAZF1 might firstly upregulated the expression of PPARalpha and PPARbeta/delta, which in turn activated the transcription of visfatin PMID: 24930994
- Specific ablation of Nampt in adult neural stem cells recapitulates their functional defects during aging. PMID: 24811750
- miR-34a reduces NAD(+) levels and SIRT1 activity by targeting NAMPT, the rate-limiting enzyme for NAD(+) biosynthesis. PMID: 23834033
- forebrain excitatory neurons mainly use intracellular Nampt-mediated NAD(+) biosynthesis to mediate their survival and function. PMID: 24760840
- GLP-1 promotes the novel insulin-mimetic adipocytokine visfatin expression. PMID: 23670349
- These results suggested that diet-induced obesity could promote murine gastric cancer growth by upregulating the expression of the nampt, sirt1 and c-myc genes. PMID: 23970286
- melatonin suppresses visfatin-induced iNOS upregulation in macrophages by inhibiting the STAT3 and NF-kappaB pathways. PMID: 23869429
- Rev-erb alpha is a new intracellular regulator of glucagon secretion via AMPK/Nampt/Sirt1 pathway. PMID: 23936124
- Nicotinamide phosphoribosyltransferase/visfatin expression by inflammatory monocytes mediates arthritis pathogenesis. PMID: 23313810
- Osteogenic differentiation correlates with intracellular nicotinamide adenine dinucleotide (NAD) metabolism in which Nampt plays a regulatory role. PMID: 23537654
- Nampt inhibition appears as a new strategy to dampen CXCL2-induced neutrophil recruitment and thereby reduce neutrophil-mediated tissue injury in mice PMID: 22452634
- cardiomyocytes are capable of secreting Nampt during stress, and exogenous Nampt is a positive regulator of cardiac hypertrophy and adverse ventricular remodeling PMID: 23203961
- the proinflammatory actions of visfatin in chondrocytes involve regulation of IR signaling pathways, possibly through the control of Nampt enzymatic activity PMID: 22399297
- This study indicates that plasma visfatin levels are significantly higher in ST-elevation myocardial infarction patients. PMID: 22137121
- Visfatin administration during superovulation plays an important role in regulating oocyte quality and can improve oocyte quality and fertility of aged female mice. PMID: 22425197
- Knockdown of Nampt in the mouse mesenchymal cell line C3H10T1/2 cells resulted in decreased Sirt1 activity and enhanced adipogenesis. PMID: 21812028
- FoxOs control the Nampt gene expression and the NAD(+) signaling in the regulation of hepatic triglyceride homeostasis PMID: 21388966
- study shows that PBEF has a neuronal protective role in cerebral ischemia presumably through enhanced energy metabolism PMID: 20485294
- analysis of inhibition of nicotinamide phosphoribosyltransferase and how cellular bioenergetics reveals a mitochondrial insensitive NAD pool PMID: 20724478
- study examined the effect of visfatin on cellular stress responses and signalling pathways in skeletal muscle; findings provide novel insights for the unique pathophysiological role of visfatin in skeletal muscle PMID: 19898975
- activated, but not resting, T lymphocytes undergo massive NAD(+) depletion upon FK866-mediated Nampt inhibition PMID: 19936064
- Estriol, estradiol and progesterone exert a synergistic effect on visfatin gene expression. Visfatin may play a physiological role during pregnancy. PMID: 20004694
- Data show that visfatin exists in all the detected tissues, with the expression in spleen and adipose tissue higher than the others. PMID: 19500503
- The role of Nampt in regulating autophagy and potential mechanisms by which NAD(+) regulates autophagy in the heart, is discussed. PMID: 19855187
- Pre-B-cell colony-enhancing factor, whose expression is up-regulated in activated lymphocytes, is a nicotinamide phosphoribosyltransferase, a cytosolic enzyme involved in NAD biosynthesis. PMID: 12555668
- NAD biosynthesis mediated by Nampt regulates the function of Sir2alpha and plays an important role in controlling various biological events in mammals PMID: 15381699