Recombinant Mouse Protein Wnt-4 (WNT4) Protein (His)

Name: Recombinant Mouse Protein Wnt-4 (WNT4) Protein (His)
Brand: Beta LifeScience
SKU: BLC-07498P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: Cytokines, Recombinant proteins

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Product Overview

Description	Recombinant Mouse Protein Wnt-4 (WNT4) Protein (His) is produced by our Mammalian cell expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P22724
Target Symbol	WNT4
Species	Mus musculus (Mouse)
Expression System	Mammalian cell
Tag	C-6His
Target Protein Sequence	SNWLYLAKLSSVGSISEEETCEKLKGLIQRQVQMCKRNLEVMDSVRRGAQLAIEECQYQFRNRRWNCSTLDSLPVFGKVVTQGTREAAFVYAISSAGVAFAVTRACSSGELEKCGCDRTVHGVSPQGFQWSGCSDNIAYGVAFSQSFVDVRERSKGASSSRALMNLHNNEAGRKAILTHMRVECKCHGVSGSCEVKTCWRAVPPFRQVGHALKEKFDGATEVEPRRVGSSRALVPRNAQFKPHTDEDLVYLEPSPDFCEQDIRSGVLGTRGRTCNKTSKAIDGCELLCCGRGFHTAQVELAERCGCRFHWCCFVKCRQCQRLVEMHTCR
Expression Range	23-351aa
Protein Length	Full Length of Mature Protein
Mol. Weight	38.9 kDa
Research Area	Cancer
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Ligand for members of the frizzled family of seven transmembrane receptors. Plays an important role in the embryonic development of the urogenital tract and the lung. Required for normal mesenchyme to epithelium transition during embryonic kidney development. Required for the formation of early epithelial renal vesicles during kidney development. Required for normal formation of the Mullerian duct in females, and normal levels of oocytes in the ovaries. Required for normal down-regulation of 3 beta-hydroxysteroid dehydrogenase in the ovary. Required for normal lung development and for normal patterning of trachael cartilage rings.
Subcellular Location	Secreted, extracellular space, extracellular matrix.
Protein Families	Wnt family
Database References	KEGG: mmu:22417 STRING: 10090.ENSMUSP00000036580 UniGene: Mm.20355
Tissue Specificity	In adults in lung and brain.

Gene Functions References

urinary Wnt4 could be a potential noninvasive biomarker for the early detection of tubular injury. PMID: 27600466
GLP-1 promoted adipogenesis through the modulation of the Wnt4/beta-catenin signaling pathway PMID: 27504979
Results suggest that the Wnt4 gene encodes signals that are important for various aspects of female reproductive tract development. PMID: 26721931
Our functional study revealed that WNT4 molecules were involved in regulating zygotic cleavage and early embryogenesis PMID: 26700598
Data indicate that a balance between supporting cell self-renewal and differentiation is maintained in the developing ovary by relative Wnt4 protein/beta-catenin and p27Kip1 protein (p27)/Forkhead box L2 (FOXL2) activities. PMID: 26939755
decreased expression of Wnt4 in the aging thymus may be one of the molecular triggers underlying the process of age-related thymic senescence. PMID: 26397044
Mir-29c regulates WNT4 signaling. PMID: 26484393
Wnt4 is essential to normal mammalian lung development. PMID: 26321050
RSPO1, WNT4, and beta-catenin have roles in the signaling pathway during ovarian differentiation in mice. PMID: 25187620
The Runx-1 gene can be a Wnt-4 signalling target, and that Runx-1 and Wnt-4 are mutually interdependent in their expression. PMID: 25645944
Progesterone and Wnt4 control stem cell function through a luminal-myoepithelial crosstalk with Wnt4 acting independent of progesterone receptor perinatally. PMID: 25603931
significant role in supporting thymocyte development and maturation PMID: 24768153
results suggest that Wnt4 signaling could be an attractive therapeutic target for treating osteoporosis and preventing skeletal aging PMID: 25108526
Data indicate that Wnt4 signaling is necessary during maturation of the ovarian follicle. PMID: 24371124
Data from several types of in vitro angiogenesis assays suggest that Wnt4 (but not Wnt1) is a potent inhibitor of capillary outgrowth; Wnt4 and Wnt1 appear to be secreted by vascular endothelial cells into extracellular matrix during angiogenesis. PMID: 22607657
Wnt4-null and Rspo1-null pregranulosa cells transition through a differentiated granulosa cell state prior to transdifferentiating towards a Sertoli cell fate. PMID: 24036309
Wnt4 signaling through beta-catenin stabilization is sufficient to drive spontaneous myofibroblast differentiation in interstitial pericytes and fibroblasts, emphasizing the importance of this pathway in renal fibrosis. PMID: 23766539
Endocardial to myocardial notch1-wnt4-bmp2 axis regulates early heart valve development. PMID: 23560082
this study identifies Rspo1 and Wnt4 as two new regulators of cell proliferation in the early gonad regardless of its sex, in addition to the specific role of these genes in ovarian differentiation. PMID: 23095882
The repression of many female genes including Wnt4 is necessary for the bipotential gonad to adopt the male testicular fate. PMID: 22705479
Our analyses show that Wt1, Sf1, and Wnt4 can be dosage-sensitive in mice, depending on the genetic background, and provide insights into the mechanisms of B6 XY sex reversal PMID: 22496664
WNT4 was found to signal via the CTNNB1 pathway in Sertoli cells. PMID: 22253774
These data reveal a new role for Wnt4 in mammalian Neuromuscular junction formation. PMID: 22253844
Wnt4 regulates steady-state thymic cellularity by a thymic epithelial cell (TEC)-dependent mechanism. The absence of Wnt4 suppressed fetal and postnatal thymic expansion and resulted in decreased TEC numbers. PMID: 21937690
Wnt-4 modulates canonical Wnt signaling and acts as regulator of beta-cell proliferation and inflammatory cytokine release. PMID: 21771967
An important role of Wnt-4 in thymic senescence, is reported. PMID: 21453014
Activation of canonical Wnt signaling was evident as indicated by increased Wnt4 activation in livers of hepatocellular carcinoma farnesoid X-knockout mice. PMID: 21430080
These results establish a functional role for non-canonical Wnt signaling in hematopoiesis. PMID: 21541287
Wnt4 activated the canonical beta-catenin pathway during myogenic differentiation in both myoblasts and satellite cells and negatively regulated myostatin's expression and its regulating pathways. PMID: 21248078
Maintenance of female germ cells hinge upon a delicate balance between positive (WNT4 and beta-catenin) and negative (activin betaB) regulators derived from the somatic cells in the fetal ovary. PMID: 20454446
WNT4 is a critical regulator not only of proper postnatal uterine development, but also embryo implantation and decidualization. PMID: 21163860
Wnt/beta-catenin signaling occurs in proliferating vascular smooth muscle cells during intimal thickening. This is a result of Wnt4 upregulation. PMID: 21193738
Wnt-4 deficiency allows 20% of the germ cells to initiate meiosis in the ovary, whereas meiosis is inhibited completely in the Wnt-4/Wnt-5a double mutant. PMID: 20106871
WNT4 is required for normal antral follicle development and may act by regulating granulosa cell functions including steroidogenesis. PMID: 20371632
Notch2 overexpression leads to ectopic Wnt4 expression and premature tubule formation. PMID: 20299358
data indicate that Wnt4 levels must be regulated in chondrocytes for normal growth plate development and skeletogenesis PMID: 17505543
The current results support a view that WNT4 may have a role in oocyte selection and follicle formation and maturation in human ovaries. PMID: 19962424
Wnt4(EGFPCre)-mediated inactivation of Wnt4 function considerably reduced the amount of Wnt4 transcripts, led to a severe defect in kidney development, and caused the female embryos to undergo partial sex reversal to males PMID: 19830824
role for Wnt-4 in the pathogenesis of renal fibrosis. PMID: 11832423
wnt-4 is expressed at distinct stages of follicular development. PMID: 11861511
results point to a role for Wnt-4 in adrenal gland development and function PMID: 12399432
Wnt4 signaling mediates the increased expression of Dax-1 as the ovary becomes sexually differentiated PMID: 12554773
Wnt-4 dependent signaling cascades regulate steroidogenic factor-1-dependent transcription of genes in adrenal cortex PMID: 12732619
Data show that the role of WNT4 in gonad development is to pattern the sex-specific vasculature and to regulate steroidogenic cell recruitment. PMID: 12835383
Wnt4 mRNA is expressed in a decreasing anterior-to-posterior gradient in the floor plate; commissural axons lacking the Wnt receptor Frizzled3 displayed anterior-posterior guidance defects after midline crossing PMID: 14671310
matrilysin may have a role in renal tubular injury and progression of tubulointerstitial fibrosis, and Wnt4 may regulate matrilysin expression in the kidney PMID: 15149334
germ cells in the ovarian cortex are almost completely lost in both Wnt4 and follistatin null gonads before birth PMID: 15162500
Wnt-4 binds both canonical and noncanonical Frizzled receptors, thereby activating Wnt signaling pathways that may each contribute to kidney tubulogenesis. PMID: 15265686
Results identify WNT4 as a new factor involved in the mammalian testis determination pathway and show that genes can have a specific but distinct role in both male and female gonad development. PMID: 15581876
Wnt-4 signaling normally acts to suppress testosterone biosynthesis in the female, and that testosterone is the putative mediator of the masculinization phenotype in Wnt-4-deficient females PMID: 15932923

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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