Recombinant Mouse Proto-Oncogene Wnt-3 (WNT3) Protein (His)

Name: Recombinant Mouse Proto-Oncogene Wnt-3 (WNT3) Protein (His)
Brand: Beta LifeScience
SKU: BLC-10865P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: Cytokines, Recombinant proteins, Recombinant proteins fall special offers - full-length proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Submit an inquiry today to inquire about all available size options and prices! Connect with us via the live chat in the bottom corner to receive immediate assistance.

Product Overview

Description	Recombinant Mouse Proto-Oncogene Wnt-3 (WNT3) Protein (His) is produced by our Yeast expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P17553
Target Symbol	WNT3
Synonyms	Wnt3; Int-4; Wnt-3; Proto-oncogene Wnt-3; Proto-oncogene Int-4
Species	Mus musculus (Mouse)
Expression System	Yeast
Tag	N-6His
Target Protein Sequence	GYPIWWSLALGQQYTSLASQPLLCGSIPGLVPKQLRFCRNYIEIMPSVAEGVKLGIQECQHQFRGRRWNCTTIDDSLAIFGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGTSTICGCDSHHKGPPGEGWKWGGCSEDADFGVLVSREFADARENRPDARSAMNKHNNEAGRTTILDHMHLKCKCHGLSGSCEVKTCWWAQPDFRAIGDFLKDKYDSASEMVVEKHRESRGWVETLRAKYALFKPPTERDLVYYENSPNFCEPNPETGSFGTRDRTCNVTSHGIDGCDLLCCGRGHNTRTEKRKEKCHCVF
Expression Range	22–355aa
Protein Length	Full Length of Mature Protein
Mol. Weight	36.9kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis. Required for normal formation of the apical ectodermal ridge and for normal embryonic limb development.
Subcellular Location	Secreted, extracellular space, extracellular matrix. Secreted.
Protein Families	Wnt family
Database References	KEGG: mmu:22415 STRING: 10090.ENSMUSP00000000127 UniGene: Mm.159091
Tissue Specificity	Detected at low levels in adult brain. Dorsal portion of the neural tube, dorsal ectoderm, the branchial arches, and the limb buds.

Gene Functions References

Downregulation of Gata4 could restore the phenotype exhibited by Wnt3 downregulation in dorsal root ganglion neurons. PMID: 29567480
Here, we describe the culture protocol using Wnt-3a for successful maintenance of skin epithelial stem cells . PMID: 27032938
stem-cell membranes constitute a reservoir for Wnt proteins, while Frizzled receptor turnover and 'plasma membrane dilution' through cell division shape the epithelial Wnt3 gradient PMID: 26863187
timed secretion of wnt3 by ingrowing axons from the thalamus regulates the combinatorial composition of ribosomal proteins in developing neocortex. WNT3 further regulates the specificity of mRNA translation and development of neurons and oligodendrocytes. PMID: 26245956
this article shows that absence of Wnt3 in the posterior visceral endoderm leads to delayed formation of the primitive streak and that interplay between anterior and posterior visceral endoderm restricts the position of the primitive streak PMID: 25907228
Data indicate that functional Wnt3/beta-Catenin signaling is localized to the dorsal subventricular zone (SVZ) microdomain. PMID: 24449255
Wnt3 is part of a signaling loop that affects homeostasis of intestinal stem and Paneth cells in mice. PMID: 22922422
Wnt3 function in the epiblast is required for the maintenance but not the initiation of gastrulation in mice PMID: 23085236
These results suggest that the regulation of paracrine factors plays a critical role in hippocampal aging and neurogenesis. PMID: 21746862
Wnt-3a and Wnt-3 factors signal through the canonical Wnt/beta-catenin pathway to regulate different aspects of spinal cord neural progenitor development. PMID: 20722074
The mRNA levels of Wnt3, 6, and 9a were significantly upregulated in the intestinal epithelial cells by Salmonella. PMID: 20083111
Different permissive Wnt3/beta-catenin signaling thresholds is required for the embryonic development of head structures and adult intestinal polyposis. PMID: 20084116
WNT-3, expressed by motor neurons, acts as a retrograde signal that controls terminal arborization of muscle afferents. PMID: 12354395
Expression is regulated by beta-catenin during formation of the anteroposterior axis and the mesoderm PMID: 14623818
effects of Wnt3a are exerted through activation of the beta-catenin signal transduction pathway and do not require alternative Wnt transduction cascades PMID: 15009701
Data suggest that recruitment of Axin and Frat1 to the membrane by LRP5-triggered Wnt3 signaling leads to both Axin degradation and Frat1-mediated inhibition of glycogen synthase kinase-3. PMID: 15699046
Wnt3a and SB216763 promoted the proliferation of retinal stem cells, partly dependent on FGF2 signaling.A combination of Wnt and FGF signaling may provide a therapeutic strategy for in vitro expansion or in vivo activation of adult retinal stem cells. PMID: 16223856
Wnt3 repulsion is mediated by Ryk, expressed in a ventral-to-dorsal decreasing gradient PMID: 16280981
We further delineate the role of Wnt signaling pathway as evidenced by induction of Wnt3 and Wnt8b, stimulation of FRP-1, inhibition of GSK-B, accumulation of cytosolic beta-catenin, and transcription of target genes, including c-myc and cyclin-D1. PMID: 16522160
This study findings reveal the rich contributions of the Wnt3a lineage to a variety of brain structures and show that functionally integrated nuclei can share a molecular identity. PMID: 17701978
Wnt3 expression in the epiblast is required for induction of the primitive streak and mesoderm whereas activity in the posterior visceral endoderm is dispensable. PMID: 18028899
an internally truncated LRP5 receptor is strongly implicated in deregulated activation of the WNT/beta-catenin signaling pathway in hyperparathyroid tumors PMID: 18044981
head development is sensitive to the level of WNT3 signalling and that DKK1 is the key antagonist that modulates WNT3 activity during anterior morphogenesis. PMID: 18403408
Wnt-3a signaling regulates hemopoiesis through stromal cells with selectivity and different degrees of the effect, depending on the hemopoietic lineage in the hemopoietic microenvironment. PMID: 11441081

FAQs

Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.

Feel free to use the Chat function to initiate a live chat. Our customer representative can provide you with a quote immediately.

Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.