Recombinant Mouse VEGFR2 / KDR Protein (His Tag), Biotinylated

Name: Recombinant Mouse VEGFR2 / KDR Protein (His Tag), Biotinylated
Brand: Beta LifeScience
SKU: BLPSN-4807
Availability: InStock

Collections: Antibody / cell therapy targets, Biotinylated proteins, Car-nk targets proteins, Car-t cell therapy targets, Cytokines and growth factors, Featured biotinylated protein molecules, Growth factors and receptors, Recombinant proteins

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Product Overview

Tag	His
Host Species	Mouse
Accession	P35918
Synonym	6130401C07, Flk-1, Flk1, Krd-1, Ly73, sVEGFR-2, VEGFR-2, VEGFR2
Background	VEGFR2, also called as KDR or Flk-1, is identified as the receptor for VEGF and VEGFC and an early marker for endothelial cell progenitors, whose expression is restricted to endothelial cells in vivo. VEGFR2 was shown to be the primary signal transducer for angiogenesis and the development of pathological conditions such as cancer and diabetic retinopathy. It has been shown that VEGFR2 is expressed mainly in the endothelial cells, and the expression is upregulated in the tumor vasculature. Thus the inhibition of VEGFR2 activity and its downstream signaling are important targets for the treatment of diseases involving angiogenesis. VEGFR2 transduces the major signals for angiogenesis via its strong tyrosine kinase activity. However, unlike other representative tyrosine kinase receptors, VEGFR2 does not use the Ras pathway as a major downstream signaling but rather uses the phospholipase C-protein kinase C pathway to signal mitogen-activated protein (MAP)-kinase activation and DNA synthesis. VEGFR2 is a direct and major signal transducer for pathological angiogenesis, including cancer and diabetic retinopathy, in cooperation with many other signaling partners; thus, VEGFR2 and its downstream signaling appear to be critical targets for the suppression of these diseases. VEGF and VEGFR2-mediated survival signaling is critical to endothelial cell survival, maintenance of the vasculature and alveolar structure and regeneration of lung tissue. Reduced VEGF and VEGFR2 expression in emphysematous lungs has been linked to increased endothelial cell death and vascular regression.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy
Description	A DNA sequence encoding the mouse KDR (P35918-1) extracellular domain (Met1-Glu762) was expressed with a C-terminal His tag. The purified protein was biotinylated in vitro.
Source	HEK293
Predicted N Terminal	Ala 20
AA Sequence	Met1-Glu762
Molecular Weight	The recombinant mouse KDR consists of 754 a.a. and predicts a molecular mass of 84.5 KDa.
Purity	>90% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg protein as determined by the LAL method.
Bioactivity	1.Measured by its binding ability in a functional ELISA. 2.Immobilized human VEGF165 at 10ug/mL (100uL/well) can bind biotinylated mouse KDR, the EC50 of biotinylated mouse KDR is 40-130ng/mL.
Formulation	Lyophilized from sterile PBS..
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.