Recombinant Pig T-Cell Surface Glycoprotein Cd3 Epsilon Chain (CD3E) Protein (His)
Beta LifeScience
SKU/CAT #: BLC-00314P
Greater than 90% as determined by SDS-PAGE.
Recombinant Pig T-Cell Surface Glycoprotein Cd3 Epsilon Chain (CD3E) Protein (His)
Beta LifeScience
SKU/CAT #: BLC-00314P
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
| Description | Recombinant Pig T-Cell Surface Glycoprotein Cd3 Epsilon Chain (CD3E) Protein (His) is produced by our E.coli expression system. This is a protein fragment. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Uniprotkb | Q7YRN2 |
| Target Symbol | CD3E |
| Species | Sus scrofa (Pig) |
| Expression System | E.coli |
| Tag | N-6His |
| Target Protein Sequence | QEDIERPDEDTQKTFKVSISGDKVELTCPEDPESEKMTWKRNDMQIYESYDNYMLLESFSEVENSGYYTCTVGEKTSHRLYLKARVCENCVEVDL |
| Expression Range | 22-116aa |
| Protein Length | Partial |
| Mol. Weight | 15.2 kDa |
| Research Area | Others |
| Form | Liquid or Lyophilized powder |
| Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Reconstitution | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Storage | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Target Details
| Target Function | Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region. |
| Subcellular Location | Cell membrane; Single-pass type I membrane protein. |
| Database References |
Gene Functions References
- expressed and refolded porcine CD3 ectodomain molecules include CD3epsilon, CD3gamma, CD3delta, CD3epsilongamma heterodimer, CD3epsilondelta heterodimer, CD3epsilongamma single-chain fusion protein and CD3epsilondelta single-chain fusion protein PMID: 22672968
