Recombinant Rat CD6 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1064
Recombinant Rat CD6 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1064
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Rat |
Accession | Q812A4 |
Background | T-cell differentiation antigen CD6, also known as TP12 and CD6, is a single-pass type I membrane protein which contains threeSRCR domains. CD6 / TP12 is a cell surface glycoprotein expressed primarily on T cells, it may function as a costimulatory molecule and may play a role in autoreactive immune responses. CD6 / TP12 is expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain. CD6 ligand termed CD166 (previously known as activated leukocyte cell adhesion molecule, ALCAM ) has been identified and shown to be expressed on activated T cells, B cells, thymic epithelium, keratinocytes, and in rheumatoid arthritis synovial tissue. CD6 / TP12 binds to activated leukocyte cell adhesion molecule ( CD166 ), and is considered as a costimulatory molecule involved in lymphocyte activation and thymocyte development. CD6 / TP12 partially associates with the TCR / CD3 complex and colocalizes with it at the center of the mature immunological synapse (IS) on T lymphocytes. During thymic development CD6-dependent signals may contribute both to thymocyte survival, and to the overall functional avidity of selection in both man and mouse. |
Description | A DNA sequence encoding the rat CD6 (Q812A4) (Met1-Asn396) was expressed with a His tag at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Leu 18 |
AA Sequence | Met1-Asn396 |
Molecular Weight | The recombinant rat CD6 comprises 390 a.a. and predicts a molecular mass of 42.5 kDa. The apparent molecular mass of the recombinant protein is approximately 64-70 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
Purity | >95% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | 1. Immobilized rat CD6-His at 10 ug/mL (100 uL/well) can bind rat ALCAM-Fc3, The EC50 of rat ALCAM-Fc3 is 19.5-45.5 ng/ml.2. Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.When 8 x 104 cells/well are added to ratCD6-His coated plates (10ug/mL, 100 uL/well), approximately 39.2% will adhere after 60 minutes at 37°C. |
Formulation | Lyophilized from sterile PBS, pH 7.4.. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |