Recombinant Rat Eotaxin Protein (CCL11), Active

Name: Recombinant Rat Eotaxin Protein (CCL11), Active
Brand: Beta LifeScience
SKU: BLC-05448P
Availability: InStock

Collections: Antibody / cell therapy targets, Antibody therapeutic / adc target proteins, Cytokines, Cytokines and growth factors, Growth factors and receptors, Recombinant proteins

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Product Overview

Description	Recombinant Rat Eotaxin Protein (CCL11), Active is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 96% as determined by SDS-PAGE and HPLC.
Endotoxin	Less than 1.0 EU/μg as determined by LAL method.
Activity	Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using purified eosinophils is in a concentration range of 0.1-1.0 μg/ml.
Uniprotkb	P97545
Target Symbol	CCL11
Synonyms	Ccl11; Scya11Eotaxin; C-C motif chemokine 11; Eosinophil chemotactic protein; Small-inducible cytokine A11
Species	Rattus norvegicus (Rat)
Expression System	E.coli
Tag	Tag-Free
Complete Sequence	HPGSIPTSCC FTMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTKLGKEIC ADPKKKWVQD ATKHLDQKLQ TPKP
Expression Range	24-97aa
Protein Length	Full Length of Mature Protein
Mol. Weight	8.4 kDa
Research Area	Immunology
Form	Lyophilized powder
Buffer	Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils (a prominent feature of allergic inflammatory reactions), but not lymphocytes, macrophages or neutrophils. Binds to CCR3.
Subcellular Location	Secreted.
Protein Families	Intercrine beta (chemokine CC) family
Database References	KEGG: rno:29397 STRING: 10116.ENSRNOP00000009756 UniGene: Rn.10632

Gene Functions References

our data suggest that the CCL11-CCR3 interaction mainly activates PI3K/Akt signal transduction pathway in HUVECs. PMID: 28279120
Iddm30 controls pancreatic expression of Ccl11 (Eotaxin) and the Th1/Th2 balance within the insulitic lesions. PMID: 24646746
Tripterine can inhibit expression of Eotaxin by restraining the activation of NF-kappaB in the nasal musoca of rats with allergic rhinitis. PMID: 23272499
expression of Ccl11 associates with immune response modulation in experimental autoimmune encephalomyelitis PMID: 22815714
Intervention of PRMT1 activity can abrogate IL-4-dependent eotaxin-1 production to influence the pulmonary inflammation with eosinophil infiltration. PMID: 22387551
The expression of Eotaxin in nasal lavage fluid and nasal mucosa increased after treatment of allergic rhinitis with histamine. PMID: 20359111
The expression of Eotaxin in nasal mucosa increased, and the expression of CCR3 in bone marrow increased in the model of allergic rhinitis. PMID: 16722399
Suppression of eotaxin by CpG DNA reduced the symptoms of experimental allergic rhinitis. PMID: 16838745
Targeting CCL11 with monoclonal antibodies possibly results in decreased myocardial fibrosis and improves contractile function after heart transplantation ub rats. PMID: 20832583
Galectin-3 inhibited expression of eotaxin and IL5, potentially suppressing eosinophilic inflammation in asthma. PMID: 17074272
Eotaxin is upregulated in the pancreatic lymph nodes and may play a role in early disease pathogenesis of type 1 diabetes mellitus in the BioBreeding rat model. PMID: 15557196
In a model of ongoing acute heart transplant rejection in the rat, we found eotaxin mRNA expression within infiltrating macrophages, but not in mast cells PMID: 19631640

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.