Recombinant Rat FGFR4 Protein (Fc Tag)

Name: Recombinant Rat FGFR4 Protein (Fc Tag)
Brand: Beta LifeScience
SKU: BLPSN-2082
Availability: InStock

Collections: Cytokines and growth factors, Enzymes, Growth factors and receptors, Kinase, Recombinant proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Submit an inquiry today to inquire about all available size options and prices! Connect with us via the live chat in the bottom corner to receive immediate assistance.

Product Overview

Tag	Fc
Host Species	Rat
Accession	Q498D6
Synonym	FGFR4, CD334, JTK2, TKF, fibroblast growth factor receptor 4
Background	Fibroblast growth factor receptor 4 is a protein that in humans is encoded by the FGFR4 gene. FGFR4 has also been designated as CD334 (cluster of differentiation 334).FGFR4 (Fibroblast Growth Factor Receptor 4) is a Protein Coding gene. Diseases associated with FGFR4 include Prostate Cancer and Rhabdomyosarcoma. Among its related pathways are PI3K/AKT activation and RET signaling. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is FGFR3.
Description	A DNA sequence encoding the rat FGFR4 (Q498D6) extracellular domain (Met 1-Asp 367) was fused with the Fc region of human IgG1 at the C-terminus.
Source	HEK293
Predicted N Terminal	Phe 17
AA Sequence	Met 1-Asp 367
Molecular Weight	The recombinant rat FGFR4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 592 amino acids and predicts a molecular mass of 66 kDa. The apparent molecular mass of the rat FGFR4/Fc monomer is approximately 110 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Purity	>90% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	1. Measured by its binding ability in a functional ELISA.2. Immobilized human FGF18 at 10 ug/mL (100 ul/well) can bind- Rat FGFR4, The EC50 of Rat FGFR4 is 1.17 ug/mL.3. Immobilized mouse FGF18 at 10 ug/mL (100 ul/well) can bind- Rat FGFR4, The EC50 of Rat FGFR4 is 0.44 ug/mL.4. Immobilized human bFGF at 10 ug/mL (100 ul/well) can bind- Rat FGFR4, The EC50 of Rat FGFR4 is 0.163 ug/mL.
Formulation	Recombinant Rat FGFR4 Protein (Fc Tag) was lyophilized from sterile PBS, pH 7.4
Stability	Recombinant Rat FGFR4 protein is stable up to 1 year at -70°C
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

FAQs

Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.

Feel free to use the Chat function to initiate a live chat. Our customer representative can provide you with a quote immediately.

Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.