Recombinant Rat Neural Cell Adhesion Molecule 1 (NCAM1) Protein (His&Myc)

Name: Recombinant Rat Neural Cell Adhesion Molecule 1 (NCAM1) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-02908P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: Antibody / cell therapy targets, Antibody therapeutic / adc target proteins, Recombinant proteins

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Product Overview

Description	Recombinant Rat Neural Cell Adhesion Molecule 1 (NCAM1) Protein (His&Myc) is produced by our E.coli expression system. This is a extracellular protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P13596
Target Symbol	NCAM1
Synonyms	Ncam1; Ncam; Neural cell adhesion molecule 1; N-CAM-1; NCAM-1; CD antigen CD56
Species	Rattus norvegicus (Rat)
Expression System	E.coli
Tag	N-10His&C-Myc
Target Protein Sequence	LQVDIVPSQGEISVGESKFFLCQVAGDAKDKDISWFSPNGEKLSPNQQRISVVWNDDDSSTLTIYNANIDDAGIYKCVVTAEDGTQSEATVNVKIFQKLMFKNAPTPQEFKEGEDAVIVCDVVSSLPPTIIWKHKGRDVILKKDVRFIVLSNNYLQIRGIKKTDEGTYRCEGRILARGEINFKDIQVIVNVPPTVQARQSIVNATANLGQSVTLVCDADGFPEPTMSWTKDGEPIENEEEDDEKHIFSDDSSELTIRNVDKNDEAEYVCIAENKAGEQDASIHLKVFAKPKITYVENQTAMELEEQVTLTCEASGDPIPSITWRTSTRNISSEEKASWTRPEKQETLDGHMVVRSHARVSSLTLKSIQYTDAGEYICTASNTIGQDSQSMYLEVQYAPKLQGPVAVYTWEGNQVNITCEVFAYPSATISWFRDGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNCTAVNRIGQESLEFILVQADTPSSPSIDRVEPYSSTAQVQFDEPEATGGVPILKYKAEWKSLGEEAWHSKWYDAKEANMEGIVTIMGLKPETRYAVRLAALNGKGLGEISAATEFKTQPVREPSAPKLEGQMGEDGNSIKVNLIKQDDGGSPIRHYLVKYRALASEWKPEIRLPSGSDHVMLKSLDWNAEYEVYVVAENQQGKSKAAHFVFRTSAQPTAIPANGSPTAGLST
Expression Range	20-721aa
Protein Length	Extracellular Domain
Mol. Weight	82.7kDa
Research Area	Neuroscience
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
Subcellular Location	Cell membrane; Single-pass type I membrane protein.
Database References	KEGG: rno:24586 STRING: 10116.ENSRNOP00000048442 UniGene: Rn.11283

Gene Functions References

Our results strongly support the hypothesis that PSA-NCAMs play a proaggregatory role in the fasciculatory process PMID: 23963795
CD56(140kD) up-regulation plays a pivotal role in the pathogenesis of ischemic cardiomyopathy. PMID: 23462508
Stressed animals exhibited an increase in NCAM mRNA expression in the hippocampus, but not in the prefrontal cortex. The synaptosomal NCAM of the hippocampus was markedly polysialylated, while cortical PSA-NCAM was significantly decreased. PMID: 22814229
A serine-to-alanine exchange at position 774 of NCAM resulted in decreased activation of the cAMP response element binding protein and decreased neurite outgrowth after NCAM stimulation in neuroblastoma cells PMID: 22419107
FGL triggers a long-lasting enhancement of synaptic transmission and long-term potentiation in hippocampal CA1 neurons by a facilitated synaptic delivery of AMPA receptors. PMID: 22363206
NCAM-derived peptide plannexin can protect immature neurons in vivo; however, in a model of status epilepticus plannexin-treated animals do not reveal any neuroprotective effects of plannexin on mature neurons. PMID: 21787839
Treatment with triiodothyronine downregulates expression of NCAM in the seminiferous tubules of rats with flutamide-induced cryptorchidism. PMID: 22007488
Polysialylated-NCAM expression is important for the connectivity of interneurons in the adult cerebral cortex; its regulation may play an important role in the structural plasticity of inhibitory networks. PMID: 20843898
Plannexin facilitates neurite outgrowth in primary hippocampal neuronal cultures and improves spatial learning under conditions involving a deficit in a key plasticity-promoting posttranslational modification of NCAM, its polysialylation. PMID: 21887252
We suggest that the expression pattern of various FGFR isoforms determines the cell context-specific effects of NCAM signaling PMID: 21876469
N-CAM is influenced by an intentionally misdirected outgrowth of motor and sensory axons following nerve repair. PMID: 20622691
NCAM is a cardioprotective factor up-regulated under metabolic stress in cardiomyocytes PMID: 19853610
Results indicate that the phosphorylation-dependent interaction between 14-3-3beta and alphaII spectrin acts as a switch between positive and negative regulation of neurite outgrowth stimulated by NCAM. PMID: 20598904
a decisive role for the neuronal K(+) channel in regulating NCAM-dependent neurite outgrowth and attribute a physiologically meaningful role to the functional interplay of Kir3.3, NCAM, and TrkB in ontogeny PMID: 20610389
Kidney cell proliferation, assessed by BrdU and PCNA labeling, was prominent in outer medulla and reach a maximum between 24 and 72 hours after reperfusion. This population was characterized by the coexpression of vimentin and nestin. PMID: 20617137
the activation of FRS2alpha by FGL was significantly lower than the activation by FGF1, indicating a differential signaling profile induced by NCAM compared with the cognate growth factor PMID: 20175207
results suggest a positive role of NCAM in neurogenesis in the periventricular region; expression of NCAM in stem cells might be one of many factors useful for therapeutic approaches in the future PMID: 19788570
microdomains responsive to NCAM-mediated signaling from growth cones. PMID: 11817895
distribution of the NCAM immunoreactivity in the sciatic nerves of 10- and 15-day-old Wistar rats PMID: 11936192
Reciprocal actions of neural cell adhesion molecule and tissue plasminogen activator via a Ras-dependent mitogen-activated protein kinase signaling pathway in hippocampal neurons. PMID: 12387826
The effect of exposure to restraint stress on NCAM mRNA was evaluated in the hippocampus. A decrease in NCAM mRNA was found throughout all hippocampal subregions. Stress may thus induce structural alterations in the the hippocampus. PMID: 12453492
Integrin alphav and NCAM mediate the effects of GDNF on DA neuron survival, outgrowth, DA turnover and motor activity in rats. PMID: 12493556
Polysialic acid NCAM expression appears first in the developing inner hair cells (IHCs) of the cochlea and then in the neighboring Kolliker's organ in an increasingly mediolateral gradient. PMID: 12640664
results showed that the protein concentration of the NCAM-140 isoform decreased in the hippocampus of stressed rats PMID: 12757368
Loss or inactivation of the polysialyl tail of NCAM reduces the migration of mechanically wounded oligodendrocyte progenitor-like (O-2A) cells into the injured area and completely blocks the wound closure. PMID: 12774308
Removal of PSA from NCAM by the enzyme Endo N leads to a significant loss of both VP and OT neurons PMID: 12786978
direct interaction between NCAM and fibroblast growth factor receptor 1 (Ig modules 2 and 3) by surface plasmon resonance analysis PMID: 12791257
Association of NCAM with GFRalpha1 downregulates NCAM-mediated cell adhesion and promotes GDNF binding to NCAM, resulting in rapid activation of cytoplasmic protein tyrosine kinases Fyn and FAK. PMID: 12837245
Strong overexpression of NCAM(CD56) and RUNX1(AML1) is a constant and characteristic feature of cardiomyocytes within or adjacent to scars in ICM. PMID: 12937148
Traumatic fear conditioning suppresses NCAM-PSA-mediated plasticity and the concomitant inability to store the trace of recently acquired information. PMID: 13129654
neural cell adhesion molecule is associated with major components of the cytoskeleton PMID: 14550299
The most intense polysialylated NCAM immunoreactivity is localized in the medial and triangular septal nuclei, regions of the brain that regulate hippocampal theta rhythm during memory consolidation. PMID: 14648585
demonstrated for the first time that IUGR newborn rat kidneys express N-CAM and cadherin adhesion molecules at specific sites of the nephron. PMID: 14663363
Amygdaloid-kindling increases polysialylated NCAM-immunoreactive mossy fibers and their terminals in the stratum lucidum of the hippocampal CA3 region and in the granule cells of the dentate gyrus. PMID: 14741393
The circulating count of CD56(dim) natural killer cells does not change during exercise training. PMID: 14752119
12 h posttraining, immunohistochemical analyses revealed a learning-induced up-regulation of polysialylated neural cell adhesion molecule in the dentate gyrus related to the spatial learning abilities displayed by rats during training. PMID: 15169853
Relevance of NCAM for tumor cell binding was proven by treating neuroblastoma cells with NCAM antisense oligonucleotides or NCAM transfection. PMID: 15256054
We therefore suggest that early synaptic NCAM expression may be one of the factors determining the capability of rats to learn. PMID: 15490465
Several interacting partners of cytosolic domains of rat brain neural cell adhesion molecule (NCAM) isoforms 140 and 180 have been identified that may play a role in NCAM-mediated signal transduction. PMID: 15865439
Results suggested that the expressions of NCAM, M-cadherin and beta-catenin in skeletal muscle satellite cells may be controlled by distinct regulatory mechanisms during functional overload and muscle hypertrophy. PMID: 16776666
Decreased NCAM-1 expression in CA1 and CA3 fields of the hippocampus, and in a lesser degree in the basal ganglia, limbic structure and cervical spinal cord in autoimmune encephalitis PMID: 17064783
upregulation of NCAM is a correlate of fear conditioning that is not necessary for the establishment of fear memory in the amygdala, but participates in mechanisms precluding fear extinction PMID: 17223582
These findings show evidence of a selective neuronal expression of PSA-NCAM in developing thalamus, supporting its suggested role in cell migration and synaptogenesis as it occurs in the cerebral cortex PMID: 17292800
The upregulation of PSA-NCAM by hyperthermia may have a significant impact on hippocampal plasticity, permitting induction of the complex molecular cascade responsible for neuroprotection. PMID: 17336079
After a dose of a CB1 receptor agonist the number of PSA-NCAM immunoreactive (IR) cells in the dentate gyrus declined in a time-dependent manner. PMID: 17355876
Exposure to stressors during juvenility alters NCAM expression and potentially underlies the predisposition to exhibit stress-related symptoms in adulthood. PMID: 17429411
upregulation of PSA-NCAM expression in the dorsal (but not ventral) dentate gyrus at 24 h after contextual (but not tone) fear conditioning PMID: 17460068
modulation of PSA-NCAM function may promote neuroregeneration in different central nervous system insults PMID: 17513116
Fluoride causes oxidative stress, apoptosis, and decreased mRNA and protein expression levels of NCAM in rat hippocampal neurons. PMID: 17537562
Given the role of PSA-NCAM in neuronal plasticity, the present results indicate that, even during aging, many regions of the CNS may display neurite, spine or synaptic remodeling. PMID: 17904697

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Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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