Recombinant Rat Neurotrophin-3 (NTF3) Protein (His&Myc)

Name: Recombinant Rat Neurotrophin-3 (NTF3) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-00729P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: Cytokines, Recombinant proteins, Recombinant proteins fall special offers - full-length proteins

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Product Overview

Description	Recombinant Rat Neurotrophin-3 (NTF3) Protein (His&Myc) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	P18280
Target Symbol	NTF3
Synonyms	(NT-3)(HDNF)(Nerve growth factor 2)(NGF-2)(Neurotrophic factor)
Species	Rattus norvegicus (Rat)
Expression System	E.coli
Tag	N-10His&C-Myc
Target Protein Sequence	YAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT
Expression Range	140-258aa
Protein Length	Full Length of Mature Protein
Mol. Weight	21.1 kDa
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Seems to promote the survival of visceral and proprioceptive sensory neurons.
Subcellular Location	Secreted.
Protein Families	NGF-beta family
Database References	KEGG: rno:81737 STRING: 10116.ENSRNOP00000026685 UniGene: Rn.9715
Tissue Specificity	Brain and peripheral tissues.

Gene Functions References

the combined delivery of NT-3 and anti-NogoA from the DDS in an impact/compression model of SCI increases axon density and improves locomotor function. PMID: 27056081
Compared to the sham group, the expression levels of NT-3 were significantly decreased and electroacupuncture was shown to upregulate the expression of NT-3 PMID: 27597902
results demonstrate that BDNF and NT3 regulate axon initial segmen location and neuronal excitability PMID: 28440877
Neurotrophin 3 activity in spinal cord was higher in balance and coordination trained animals following sciatic nerve crush compared to endurance trained. PMID: 26316168
The expressed BDNF and NT3 exert a synergistic effect, which may promote the neuronal differentiation of adiposederived stem cells. PMID: 26239042
These results indicate the feasibility of fabricating NT-3-immobilized scaffolds using the adsorption of NT-3/SF coating method, as well as the potential of these scaffolds to induce SCI repair by promoting survival and neuronal differentiation PMID: 25215612
NT-3 gene-modified bone marrow-derived mesenchymal stem cells via lumbar puncture can strengthen the therapeutic benefits of bone mesenchymal stem cell transplantation PMID: 24744011
A method of hypoglossal-facial nerve anastomosis through an NT3-expressing predegenerated nerve graft engineered by lentiviral vectors is designed to treat complete facial palsy in adult rats. PMID: 23581595
Our results show that NT-3 modifies the ability of the mossy fiber pathway to present subsequent long-term potentiation PMID: 23387385
mRNA expression of brain-derived neurotrophic factor (BDNF) and NT-3 (but not nerve growth factor) in injury segment follows neural stem cell transplantation and is remarkably upregulated. PMID: 22573254
The exposure to long-term stress likely affects a variety of organs capable of releasing NT-3 into the bloodstream. Elevation of plasma NT-3 levels may play important roles in homeostasis under stress conditions. PMID: 23074106
We conclude that upregulation of NT-3 in neonatally wounded skin is a critical factor mediating the sensory nerve sprouting that underlies hypersensitivity and pain following skin injury. PMID: 22871470
Upregulation of neurotrophin-3 in PC12 cells reduces apoptosis under hypoxic conditions. PMID: 22960790
The NTF3 gene promoted differentiation of neural stem cells into cholinergic neurons and enhanced neuronal cell survival PMID: 22971496
Accompanying the simulated weightlessness extension, NT-3 expression in rat soleus muscle spindles is progressively decreased. PMID: 21340438
Transient receptor potential canonical (TRPC)5 acts as a novel and specific mediator for NT-3 to regulate dendrite development through calcium/calmodulin-dependent protein kinase (CaMK)IIalpha. PMID: 22764246
Muscle NT-3 levels increased by exercise training contribute to the improvement in caudal nerve conduction velocity in diabetic rats. PMID: 22552353
Endogenous NT-3 may be involved in the analgesic effect of electroacupuncture on inflammatory pain in rats, mediated through the inhibition of spinal glial activity. PMID: 21680256
Neurotrophin 3 modulates neuropathic pain in the dorsal root ganglia in a rat model. PMID: 21221027
MiR21 is a candidate for regulating neurotrophin-3 signaling in the hippocampus following status epilepticus. PMID: 22019057
neurotrophin 3 signaling pathway is essential for opiate-induced adaptation of locus coeruleus neurons. PMID: 17688944
NT-3 immunoreactivity increased in activated fibers after partial transection of facial nerve. PMID: 21473141
Neurotrophin 3 may lead to enhanced exocytosis of synaptic vesicles. PMID: 21370927
regular rhythms of BDNF and NT-3 are essential for correct cortical network formation in juvenile rodents PMID: 21527636
regrowth and perception were improved when the nerve graft overexpressed neurotrophin-3 at the time of transplantation PMID: 21135740
Endogenous NT-3 has a distinctive role in promoting spiral ganglion axon growth in the organ of Corti and synaptogenesis on inner hair cells. PMID: 21613508
Results suggest that proneurotrophin-3 and proBDNF may play important roles in the response to noise-induced injuries or ototoxic damage via the Sortilin:p75(NTR) death-signalling complex. PMID: 21261755
3'UTR of calreticulin has two conserved regions, and either of these is sufficient for axonal and dendritic targeting; only nucleotides 1315-1412 show ligand responsiveness to neurotrophin 3 (NT3) and myelin-associated glycoprotein (MAG) PMID: 20308067
Report the proangiogenic capacity of NT-3 and propose NT-3 as a novel potential agent for the treatment of ischemic disease. PMID: 20360537
Our results indicate for the first time that monoaminergic neurotransmitters play an important role in the regulation of NT-3 synthesis in cultured rat astrocytes. PMID: 19854260
Brain-derived neurotrophic factor (BDNF) levels in the spinal cord, as detected by enzyme-linked immunosorbent assay, decreased with time, whereas neurotrophin-3 (NT-3) levels remained unchanged. PMID: 19203225
exercise decreases hippocampal neurotrophin-3 PMID: 12914971
ability of neurotrophin-3 (NT-3) to prevent abnormalities in neurofilamens in sensory neurons in experimental diabetes PMID: 12941778
neurotrophin-3 (NT-3), delivered chronically via fibroblasts implanted intrathecally into neonatal rats, can facilitate synaptic transmission in the spinal cord PMID: 14507970
Transfection of NT-3 into oligidendrocyte precursors caused a 10-fold increase in myelin basic protein production observed in myelin membranes in cocultures with hippocampal neurons. PMID: 15139015
the level of supraspinal and muscle afferent input plays an important role in modulating the levels of BDNF and NT-3 in the spinal cord. PMID: 15548637
These data demonstrate that localized overexpression of BDNF or NT-3, but not NGF, is sufficient to promote sprouting of serotonergic axons in the cortex following an experimental neurotoxic injury. PMID: 16206279
BDNF, NT-3, and NGF, secreted by transplanted neural stem cells, were involved in the promotion of corticospinal axon growth after transplantation PMID: 17515814
Glutamate receptor blockade inhibited the generation of spontaneous sodium-driven action potentials which, in turn, regulate both the endogenous secretion of NT-3 and the calbindin-D(28k) phenotype acquisition. PMID: 17561837
neurotrophin-3 is a unique hypertrophic factor, which is paradoxically downregulated in cardiac hypertrophy and might counteract hypertrophic change PMID: 17628607
Biochemically, the XYS decoction also reversed CIS-induced decreases in BDNF and increases in TrkB and NT-3 in the frontal cortex and the hippocampal CA(1) subregion. PMID: 17647101
Vascular-derived NT-3 increases or maintains neuropeptide Y and tyrosine hydroxylase expression in postganglionic sympathetic neurons PMID: 18032527
The functional consequences of the effect of IL-1beta on NT-3 signaling were severe, as NT-3 protection of the trophic support-deprived cortical neurons was abrogated. PMID: 18036576
Increased NT-3 mRNA expression in hippocampus was detected in the immobilization stress group; the magnitude of increase in NT-3 mRNA was decreased when animals were allowed to bite. PMID: 18191115
Co-culture of Ntf3 over-expressing Schwann cells with SGNs from early postnatal rats significantly enhanced neuronal survival in comparison to both control Schwann cells and conventional recombinant neurotrophin proteins. PMID: 18304740
Non-viral vector-mediated genetic engineering of OEG was safe and more effective in producing NT-3 and promoting axonal outgrowth followed by enhancing SCI recovery in rats. PMID: 18369383
Exposure of neurons to increased levels of NT-3 reduces expression of sodium channels Na(v)1.8 and Na(v)1.9 in dorsal root ganglia neurons subject to chronic constriction injury. PMID: 18601922
levels in cerebellar vermis were higher for animals in an enriched environment after prenatal alcohol exposure PMID: 18652597
Analysis of neurotrophin expression in dentate gyrus dosen't reveal an expression change of Ntf3 in a genetically hypertensive strain of Wistar rat. PMID: 18957307
findings suggest that orexins, but not melanin-concentrating hormone, may be an inducer of NT-3 in the cerebral cortex PMID: 19026718

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