Recombinant Rat TGFBR2 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4455
Recombinant Rat TGFBR2 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4455
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Product Overview
Tag | Fc |
Host Species | Rat |
Accession | P38438 |
Background | TGFBR2 is member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. It is a transmembrane protein. TGFBR2 is comprised by a C-terminal protein kinase domain and an N-terminal ectodomain. The ectodomain consists of a compact fold containing nine beta-strands and a single helix stabilised by a network of six intra strand disulphide bonds. The folding topology includes a central five-stranded antiparallel beta-sheet, eight-residues long at its centre, covered by a second layer consisting of two segments of two-stranded antiparallel beta-sheets. TGFBR2 has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in TGFBR2 gene have been associated with Marfan syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. TGFBR2 attenuates the biological activities of TGF-beta in colorectal cancer. TGFBR2 expression is increased in oral squamous cell carcinoma cells. Its expression is decreased by IL-1beta while inducing Sp3 via NFkappaB. TGFB2 and TGFBR2 are involved in the antiestrogenic activity. |
Description | A DNA sequence encoding the rat TGFBR2 (P38438) (Met1-Gln166) was expressed, fused with the Fc region of human IgG1 at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Ile 24 |
AA Sequence | Met1-Gln166 |
Molecular Weight | The recombinant rat TGFBR2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 384 a.a. and has a predicted molecular mass of 43 kDa. The apparent molecular mass of the protein is approximately 54 kDa in SDS-PAGE under reducing conditions. |
Purity | >95% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Please contact us for detailed information |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Target Details
Target Function | Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFRB1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways. |
Subcellular Location | Cell membrane; Single-pass type I membrane protein. Membrane raft. |
Protein Families | Protein kinase superfamily, TKL Ser/Thr protein kinase family, TGFB receptor subfamily |
Database References |
Gene Functions References
- Findings outlined in the current study demonstrated that 1,25(OH)2D3 was a promising therapeutic modality for treatment of Pulmonary arterial hypertension, function of which was exerted through miR-204 mediated Tgfbr2 signaling. PMID: 29196166
- Blocking Hsp90-Cdc37 interactions during pressure overload hypertrophy results in ubiquitin-mediated proteasomal degradation of TGFBR2 leading to termination of TGF beta mediated signaling. PMID: 26362850
- The levels of collagen I and alpha-SMA were downregulated in CS/S58 gel-treated eyes. Conjunctival fibroblast proliferation and the inflammation response were also suppressed in the CS/S58 gel-treated group. PMID: 26284552
- Morphological and biochemical data about the endosomal compartments involved in the internalization of TbetaRII upon inflammatory stimuli are shown. PMID: 25813266
- We proved that miRNA-337 is associated with chondrogenesis through regulating TGFBR2 expression, and miRNA-337 can also influence cartilage-specific gene expression in chondrocytes. PMID: 22425884
- the increased dimerization of TbetaRII in hypertrophic cardiomyocytes comparing to the normal cardiomyocytes was found. PMID: 21382347
- These data indicated that knockdown of TGFbetaRII expression inhibited the activation of hepatic stellate cells and the production of fibrogenic extracellular matrix components in HSC-T6 cells. PMID: 21378033
- Dragon's Blood can effectively reduce pulmonary fibrosis by inhibiting the expression of TGFbetaR II mRNA in the lung tissue. PMID: 17953362
- Differential expression of TGF-beta type I and II receptors by pulmonary cells in bleomycin-induced lung injury correlate with repair and fibrosis, with reduced Tgfbr2 expression occurring during the later (days 14-28 )decreased cell proliferation stage. PMID: 11936776
- TGFBRII expression is induced by acetaldehyde, a major active metabolite of alcohol PMID: 12223100
- Tgfbr2 had a marked yet transient upregulation in glomerular cells in experimental glomerulonephritis. PMID: 12545247
- detection of TGFbetas, TbetaRII expression and telomerase activity in hyperplastic, dysplastic cholangiocytes, cholangiocarcinoma cells as well as in stroma fibroblasts during cholangiocarcinogenesis PMID: 12632524
- albumin-induced internalization of TbetaRII signaling may be an important mechanism in the vessel wall for controlling TGF-beta responses in endothelial cells PMID: 14729511
- The decreased expression of TGF-beta1 mRNA in the hypoplastic heart suggests that the downregulation of RAS may be involved in the pathogenesis of cardiac hypoplasia in nitrofen-induced CDH. PMID: 15578192
- Maximal content of TGFbetaRII has been observed immunohistochemically in the early stage of pancreatic regeneration following ischemia/reperfusion-induced acute pancreatitis, suggesting the involvement of this growth factor in pancreatic recovery. PMID: 15613744
- Pituitary cells, which demonstrate reduced expression of dopamine beta2 receptor, also show reduction of TGFbeta1 type II receptor. PMID: 15961557
- Connective tissue growth factor, which is induced by TGFbeta, is expressed only iin heart transplantations with chronic rejection. PMID: 16611331
- Transforming growth factor beta receptor 2 were obviously expressed in primitive pulmonary alveolus, showing it plays an important regulatory role in the development of rat embryo and embryonic lung, especially in organic morphodifferentiation. PMID: 17428384
- transforming growth factor-beta receptor II promoter is methylated by lentivirus-mediated shRNA delivery PMID: 17533113
- Tgfbr2 was methylated and markedly down-regulated in three of seven 3,2'-dimethyl-4-aminobiphenyl-induced invasive adenocarcinomas in the dorsolateral lobe of the rat prostate. PMID: 18381416