Recombinant Rhesus IGFBP1 Protein (His Tag)

Name: Recombinant Rhesus IGFBP1 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-2573
Availability: InStock

Collections: Cytokines and growth factors, Growth factors and receptors, Recombinant proteins

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Product Overview

Tag	His
Host Species	Rhesus
Accession	XP_001085935.2
Synonym	IGFBP1 Insulin-like growth factor-binding protein 1; IBP-1; IGF-binding protein 1; IGFBP-1; Placental protein 12; PP12
Background	Insulin-like growth factor-binding protein 1 (IGFBP1) is encoded by 259 amino acid (aa) with 25 aa residue signal peptide that is processed to generate the 234 aa residue mature protein. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
Description	A DNA sequence encoding the rhesus IGFBP1 (Met23-Asn281) was expressed, fused with a His tag at the C-terminus.
Source	HEK293
Predicted N Terminal	Ala 48
AA Sequence	Met23-Asn281
Molecular Weight	The recombinant rhesus comprises 245 amino acids and has a calculated molecular mass of 26.7 KDa.
Purity	>98% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by its binding ability in a functional ELISA. Immobilized Rhesus IGFBP1-His at 10 ug/ml (100 ul/well) can bind biotinylated human IGF1 with a linear range of 12.5-100 ng/ml.
Formulation	Recombinant Rhesus IGFBP1 Protein (His Tag) was lyophilized from sterile PBS, pH 7.4
Stability	Recombinant Rhesus IGFBP1 protein is stable up to 1 year at -70°C
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.