Recombinant Rhesus IGFBP2 Protein (His Tag)

Name: Recombinant Rhesus IGFBP2 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-2576
Availability: InStock

Collections: Cytokines and growth factors, Growth factors and receptors, Recombinant proteins

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Product Overview

Tag	His
Host Species	Rhesus
Accession	XP_001087071.1
Synonym	IGFBP2, IBP2, IGF-BP53, insulin like growth factor binding protein 2
Background	IGFBP2 (Insulin Like Growth Factor Binding Protein 2) is a Protein Coding gene. Diseases associated with IGFBP2 include Insulin-Like Growth Factor I and Anorexia Nervosa. Among its related pathways are Development IGF-1 receptor signaling and Myometrial Relaxation and Contraction Pathways. Gene Ontology (GO) annotations related to this gene include insulin-like growth factor binding and insulin-like growth factor II binding. An important paralog of this gene is IGFBP4.
Description	A DNA sequence encoding the rhesus IGFBP2 (Met1-Gln324) was expressed, fused with a His tag at the C-terminus.
Source	HEK293
Predicted N Terminal	Glu 38
AA Sequence	Met1-Gln324
Molecular Weight	The recombinant rhesus IGFBP2 comprises 300 amino acids and has a calculated molecular mass of 32.8 KDa.
Purity	>67% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by its ability to inhibit the biological activity of IGFII on MCF7 human breast adenocarcinoma cells. The ED50 for this effect is typically 0.02-0.1 ug/mL in the presence of 14 ng/mL human IGFII.
Formulation	Recombinant Rhesus IGFBP2 Protein (His Tag) was lyophilized from sterile PBS, pH 7.4
Stability	Recombinant Rhesus IGFBP2 protein is stable up to 1 year at -70°C
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.