T4 Polynucleotide Kinase

Name: T4 Polynucleotide Kinase
Brand: BetaLifeSci
SKU: ENY-103
Availability: InStock

Collections: Featured enzyme molecules, Tool enzymes & other reagents

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Product Overview

Description	T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer and exchange of Pi from the Î³ position of ATP to the 5' -hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3'-monophosphates. T4 Polynucleotide Kinase also catalyzes the removal of 3'-phosphoryl groups from 3'-phosphoryl polynucleotides, deoxynucleoside 3'-monophosphates and deoxynucleoside 3'-diphosphates. T4 PNK is applicable to end-labeling DNA or RNA for probes and DNA sequencing, addition of 5'-phosphates to oligonucleotides to allow subsequent ligation and removal of 3'-phosphoryl groups.
Source	A E. coli strain that carries the cloned T4 Polynucleotide Kinase gene.
Applications	Phosphorylation (Kinase)
Size	250 U / 500 U / 2500 U
Concentration	10,000 U/ml
Components	T4 Polynucleotide Kinase (10,000 U/ml)ï¼›10X T4 PNK Reaction Buffer
Unit Definition	One Richardson unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol of acid-insoluble [32P] in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X T4 PNK Reaction Buffer with 66 μM [Î³-32P] ATP (5 x 106 cpm/μmol) and 0.26 mM 5'-hydroxyl-terminated salmon sperm DNA.
Usage	For Research Use Only
Storage	Store the T4 Polynucleotide Kinase at -20°C. Please avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.