Taq DNA Polymerase (Mg2+ Plus Buffer)

Name: Taq DNA Polymerase (Mg2+ Plus Buffer)
Brand: BetaLifeSci
SKU: ENY-107
Availability: InStock

Collections: Featured enzyme molecules, Tool enzymes & other reagents

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Description	Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5'→ 3' polymerase activity and a 5' flap endonuclease activity. Taq DNA Polymerase was isolated from a recombinant source and offers robust and reliable reactions. It tolerates a wide range of templates and incorporates dUTP, dITP and fluorescently-labeled nucleotides.
Source	An E.coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1.
Applications	Multiplex PCR, PCR , Specialty PCR, Routine PCR
Size	1000 U / 5000 U / 10000 U
Concentration	5,000 U/ml
Components	Taq DNA Polymerase (5,000 U/ml); 10X PCR Reaction Buffer, Mg2+ plus
Unit Definition	One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 75°C.
Usage	For Research Use Only
Storage	Storage Conditions: 10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Tween 20, 0.5% NP-40, 50% Glycerol, pH 7.4 @ 25°C. Store the Taq DNA Polymerase (Mg2+ Plus Buffer) at -20°C. Please avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.