Biotinylated Recombinant Arabidopsis Thaliana Abscisic Acid Receptor Pyr1 (PYR1) Protein (MBP&His-Avi)

Name: Biotinylated Recombinant Arabidopsis Thaliana Abscisic Acid Receptor Pyr1 (PYR1) Protein (MBP&His-Avi)
Brand: Beta LifeScience
SKU: BLC-07415P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: Avi-tagged proteins, Biotinylated proteins, Featured biotinylated protein molecules, Other recombinant proteins, Recombinant proteins, Recombinant proteins fall special offers - full-length proteins

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Product Overview

Description	Biotinylated Recombinant Arabidopsis Thaliana Abscisic Acid Receptor Pyr1 (PYR1) Protein (MBP&His-Avi) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	O49686
Target Symbol	PYR1
Species	Arabidopsis thaliana (Mouse-ear cress)
Expression System	E.coli
Tag	N-MBP&C-6His-Avi
Target Protein Sequence	MPSELTPEERSELKNSIAEFHTYQLDPGSCSSLHAQRIHAPPELVWSIVRRFDKPQTYKHFIKSCSVEQNFEMRVGCTRDVIVISGLPANTSTERLDILDDERRVTGFSIIGGEHRLTNYKSVTTVHRFEKENRIWTVVLESYVVDMPEGNSEDDTRMFADTVVKLNLQKLATVAEAMARNSGDGSGSQVT
Expression Range	1-191aa
Protein Length	Full Length
Mol. Weight	69.3 kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Receptor for abscisic acid (ABA) required for ABA-mediated responses such as stomatal closure and germination inhibition. Inhibits the activity of group-A protein phosphatases type 2C (PP2Cs) when activated by ABA. Can be activated by both (-)-ABA and (+)-ABA. Promotes drought tolerance.
Subcellular Location	Cytoplasm. Cytoplasm, cytosol. Nucleus. Cell membrane. Vacuole.
Protein Families	PYR/PYL/RCAR abscisic acid intracellular receptor family
Database References	KEGG: ath:AT4G17870 STRING: 3702.AT4G17870.1 UniGene: At.2045

Gene Functions References

study suggests both nuclear and cytoplasmic PYR1 participate in the control of ABA signal transduction PMID: 28109881
The transgenic plants show an acute abscisic acid (ABA) insensitivity, demonstrating the importance of ABA-mediated PYR/PYL/RCAR-PP2C contacts for receptor function in vivo. PMID: 21357183
Pyr1 receptor contributes to ozone, reduced air humidity and darkness induced stomatal regulation. PMID: 23703845
findings are generally consistent with the competitive-interaction model for PYR1 but highlight dynamical contributions of the PYR1 structure in mediating interaction selectivity PMID: 23825939
This work illustrates how oligomerization of abscisic acid receptors can modulate hormonal responses in Arabidopsis and more generally, the sensitivity of a ligand-dependent signalling system. PMID: 21847091
Subtle changes in the binding pockets of PYR/PYL proteins control ligand orientation inside the pocket, and this is sufficient to explain whether pyrabactin acts as an effective receptor agonist. [PYR1] PMID: 20729860
X-ray data is reported for PYL9/RCAR1, PYL5/RCAR8 and PYR1/RCAR11 in the presence of (+)-abscisic acid. The three proteins crystallize in space groups P3(1)21/P3(2)21, P2 and P1, respectively. X-ray intensity data were collected to 1.9-2.6 A resolution. PMID: 20383021
findings show that abscissic binds to PYRABACTIN RESISTANCE 1 (PYR1),At4g17870, which in turn binds to and inhibits Type 2C protein phosphatases (HAB1) PMID: 19407142
the crystal structure of Arabidopsis thaliana PYR1, which consists of a dimer in which one of the subunits is bound to ABA. PMID: 19898494
structure of pyrabactin resistance 1 (PYR1); structure reveals an alpha/beta helix-grip fold & homodimeric assembly; abscisic acid (ABA) binding within a large internal cavity switches structural motifs distinguishing ABA-free from ABA-bound conformations PMID: 19933100

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.