Recombinant Cynomolgus IL-23 alpha&Mouse IL-12 beta Protein, Active

Name: Recombinant Cynomolgus IL-23 alpha&Mouse IL-12 beta Protein, Active
Brand: Beta LifeScience
SKU: BLK-01939P-100UG
Availability: InStock

Cynomolgus IL-23 alpha&Mouse IL-12 beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Description	Recombinant Cynomolgus IL-23 alpha&Mouse IL-12 beta Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Val22-Pro189(Cynomolgus IL-23 alpha)&Met23-Ser335(Mouse IL-12 beta).
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Accession	A0A2K5TLV4-1(Cynomolgus IL-23 alpha)&P43432-1(Mouse IL-12 beta)
Target Symbol	IL-23 alpha&IL-12 beta
Synonyms	IL23 alpha; IL12 beta; IL23 alpha&IL12 beta
Species	Cynomolgus&Mouse
Expression System	HEK293
Tag	N-His
Expression Range	Val22-Pro189(Cynomolgus IL-23 alpha) & Met23-Ser335(Mouse IL-12 beta)
Mol. Weight	The protein has a predicted MW of 19.5 (Cynomolgus IL23 alpha)&35.8 (Mouse IL12 beta) kDa. Due to glycosylation, the protein migrates to 22 (Cynomolgus IL23 alpha)&40-50 (Mouse IL12 beta) kDa based on Tris-Bis PAGE result.
Form	Lyophilized
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Endotoxin	Less than 1EU per ug by the LAL method.
Activity	Immobilized Cynomolgus IL-23 alpha&Mouse IL-12 beta, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-23R, hFc Tag with the EC50 of 57.5ng/ml determined by ELISA (QC Test). The affinity constant of 3.90 nM as determined in SPR assay (Biacore T200). Contact us for detailed testing images.
Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Shipping	Shipped at ambient temperature.
Gene Background	Interleukin (IL)-12 and IL-23 belong to the IL-12 type family and are composite cytokines, consisting of the common β subunit p40 and the specific cytokine α subunit p35 and p19, respectively. IL-12 signals via the IL-12Rβ1·IL-12Rβ2 receptor complex, and IL-23 uses also IL-12Rβ1 but engages IL-23R as second receptor.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.