Recombinant Human AGP2 Protein (C-6His)

Name: Recombinant Human AGP2 Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-0793NP
Availability: InStock

BL-0793NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Description	Recombinant Human Alpha-1-acid Glycoprotein 2 is produced by our Mammalian expression system and the target gene encoding Gln19-Ser201 is expressed with a 6His tag at the C-terminus.
Accession	P19652
Synonym	Orosomucoid-2; OMD 2; ORM2; AGP2
Gene Background	Alpha-1-acid glycoprotein 2 is a secreted protein that belongs to the calycin superfamily, lipocalin family.It is expressed by the liver and secreted in plasma. It appears to function in modulating the activity of the immune system during the acute-phase reaction. It functions as transport protein in the blood stream. It binds various hydrophobic ligands in the interior of its beta-barrel domain. It also binds synthetic drugs and influences their distribution and availability.
Molecular Mass	22.6 KDa
Apmol Mass	25-50 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Functions as transport protein in the blood stream. Binds various hydrophobic ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability. Appears to function in modulating the activity of the immune system during the acute-phase reaction.
Subcellular Location	Secreted.
Protein Families	Calycin superfamily, Lipocalin family
Database References	HGNC: 8499 OMIM: 138610 KEGG: hsa:5005 STRING: 9606.ENSP00000394936 UniGene: Hs.719954
Tissue Specificity	Expressed by the liver and secreted in plasma.

Gene Functions References

the glycosylation change of alpha-1-acid glycoprotein (AGP) in hepatocellular carcinoma (HCC), cirrhosis and controls PMID: 28621608
Neural cell interactions are important for brain physiology and pathology. Particularly, the interaction between non-neuronal cells plays a central role in regulating brain inflammation, which is closely linked to many brain disorders. Here, we newly identified orosomucoid-2 (ORM2) as an endogenous protein that mediates such non-neuronal glial cell interactions PMID: 28193696
Low ORM2 expression is associated with metastasis in hepatocellular carcinoma. PMID: 25965830
Data suggest that human serum albumin (HSA) might serve as a carrier in delivering chitooligomers to target tissues than alpha-1-glycoprotein (AGP) which has pharmacological importance. PMID: 24359035
Alteration in expression of ORM2 suggests that ORM2 could be used as a potential biomarker in the diagnosis of colorectal cancer. PMID: 22363757
the sera of patients with acute inflammation demonstrated increased numbers of bi-antennary and alpha1,3-fucosylated N-glycan structures at each glycosylation site PMID: 15863355
The binding of coumarin enantiomers to ORM2 is studied. PMID: 16290938
Results describe the thermal unfolding and reversibility of temperature-induced changes in alpha(1)-acid glycoprotein. PMID: 16331959
The results indicate that, in accordance with prior expectations, the ORM2 variant is responsible for the acute-phase property of alpha-1 acid glycoprotein. PMID: 19018521
structure of the alpha1-acid glycoprotein (AGP), or orosomucoid (ORM), gene was investigated in a Ghanaian mother and her child, who shared an unusual variant, ORM1 S2(C), found by isoelectric focusing PMID: 11587070

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.