Recombinant Human ANG Protein

Name: Recombinant Human ANG Protein
Brand: Beta LifeScience
SKU: BL-1813NP
Availability: InStock

BL-1813NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Description	Recombinant Human Angiogenin is produced by our E.coli expression system and the target gene encoding Gln25-Pro147 is expressed.
Accession	P03950
Synonym	Angiogenin; Ribonuclease 5; RNase 5; ANG; RNASE5
Gene Background	Angiogenin belongs to the pancreatic ribonuclease family. Angiogenin is primarily expressed in the liver. It may act as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Angiogenin is a potent stimulator of new blood vessel formation. And Angiogenin is endocytosed and translocated to the nucleus by binding to actin on the surface of endothelial cells. Angiogenic activity is regulated by interaction with RNH1 in vivo. In addition, Angiogenin is associated with susceptibility to amyotrophic lateral sclerosis, which is a degenerative disorder of motor neurons in the cortex, brain stem and spinal cord.
Molecular Mass	14.27 KDa
Apmol Mass	14 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.
Subcellular Location	Cytoplasmic vesicle, secretory vesicle lumen. Secreted. Nucleus. Nucleus, nucleolus.
Protein Families	Pancreatic ribonuclease family
Database References	HGNC: 483 OMIM: 105850 KEGG: hsa:283 STRING: 9606.ENSP00000336762 UniGene: Hs.283749
Associated Diseases	Amyotrophic lateral sclerosis 9 (ALS9)
Tissue Specificity	Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons.

Gene Functions References

Angiogenin contributes to angiogenesis induced by Brain-derived neurotrophic factor (BDNF). PMID: 29573867
Data show that phospholipase D2 (PLD2)-produced phosphatidic acid (PA) promoted cell invasion through the the expression of angiogenin (ANG) in clear cell renal cell carcinoma (ccRCC) cells. PMID: 29660846
The results revealed that PCNA is predominantly localized in the cytoplasm, while hAng is distributed both in the nucleus and in the cytoplasm. hAng and PCNA colocalize in the cytoplasm, suggesting that they may interact in this compartment. PMID: 28777577
RNase 5 suppressed p27 and up-regulated cyclin D1, D3, and E by activating PI3-kinase/Akt in CECs to initiate cell cycle progression PMID: 27526633
When yeast genetic interaction partners held in common between human OPTN and ANG were validated in mammalian cells and zebrafish, MAP2K5 kinase emerged as a potential drug target for amyotrophic lateral sclerosis therapy PMID: 28596290
There was a significant association between RNase 5 and histological differentiation in colon adenocarcinomas, but no association between RNase 5 and Necl 4 in gastric or colon adenocarcinomas PMID: 28561015
Results continue to establish ANG as an oncoprotein and further reveal that ANG contributes to oncogenesis by the activation of MMP2 through modulation of DNMT3b functions. PMID: 27317771
(31) RRR(33) and (50) KRSIK(54) motifs of angiogenin might play a critical role in the regulation of p53-mediated apoptosis and angiogenesis in cancer cells. PMID: 29105754
Study shows that plexin-B2 (PLXNB2) is the functional receptor for ANG in endothelial, cancer, neuronal, and normal hematopoietic and leukemic stem and progenitor cells. PMID: 29100074
Delivery of the therapeutic human genes VEGF and ANG using an adenoviral vector improved functional recovery after traumatic spinal cord injury in a rat model. Immunofluorescent analysis of the post-treatment spinal cord suggested that the positive effect of Ad5-VEGF+Ad5-ANG transduction on recovery of locomotor function may be due to the action of glial cells on motor neurons. PMID: 28452633
Ang down-regulate the expression of Col-I, alpha-SMA and TGF-beta1/Smad2/3 and subsequently inhibits fibroblast-myofibroblast transition. PMID: 27543459
Ang,Tie1 and Tie2 play roles in vascular development and pathogenesis of vascular diseases.[review] PMID: 27941161
Results indicate that angiogenin (ANG) plays a critical role in regulating angiogenesis and follicle survival in xenografted human ovarian tissues. PMID: 28274269
we found that ANG plays an important role in EMT in squamous cell lung carcinoma and may be a valuable therapeutic target for squamous cell lung carcinoma PMID: 27667357
There is Lack of association between the Angiogenin (ANG) rs11701 polymorphism and amyotrophic lateral sclerosis risk. PMID: 26753798
ANG plays a non-cell-autonomous role in regulation of hematopoiesis by simultaneously preserving hematopoietic stem/progenitor cells stemness and promoting myeloid-restricted progenitor cell proliferation. PMID: 27518564
ANG K17I variant is rare in Caucasian patients and controls and increases the risk for amyotrophic lateral sclerosis PMID: 26255299
ANG rs11701 variant is a genetic risk factor for Parkinson's disease in Taiwan population.Mutations in ANG are not a common cause for idiopathic Parkinson's disease. PMID: 25386690
In conclusion, angiogenin secretion by HCCs favors tumor development by inducing HSC activation and ECM remodeling. PMID: 25604905
the present study established a link between Ang and FHL3 proteins and identifies a new pathway for regulating astrocytoma progression. PMID: 25659096
Studied the mechanism of ligand binding in human angiogenin using the NMR chemical shift projection analysis. PMID: 25450558
ANG could play a pivotal role in the development of bladder cancer through regulating AKT/mTOR signaling pathway. PMID: 25564356
Suggest a novel regulatory pathway whereby breast tumor-derived angiogenin directly activates angiogenesis through inhibition of miR-542-3p. PMID: 26272182
a biological explanation for the loss-of-function of D22G-Angiogenin leading to ALS, and suggest that the L35P-Angiogenin mutation would probably cause ALS symptoms in individuals harboring this mutation PMID: 25372031
High angiogenin expression is associated with bladder cancer. PMID: 24696417
a novel mechanism of ANG in regulating PI3K/AKT/mTOR signaling pathway via RI, is reported. PMID: 25193113
Up-regulated ANG expression and increased nuclear translocation were observed in cervical cancers as compared to normal cervical tissues; upregulation of ANG was positively correlated with primary tumor invasion PMID: 25552400
findings support an unrecognized interplay between ANG, ERK1/2 and MMP2 that can impact tumor growth and progression. PMID: 24561529
The observed pattern of angiogenin expression is compatible with a role in blood vessel formation and in cross-talk between trophoblasts and endothelial cells. PMID: 25093183
Serum angiogenin levels may be useful to distinguish between cancer and noncancer patients among the candidates for prostatic biopsy in regular clinical practice. PMID: 23921786
ANG interacts with plasminogen activation system at the leading edges of breast cancer cell surfaces and facilitates interactions of uPAR with uPA to regulate plasmin formation and cell migration. PMID: 24457100
Amyloidogenic ApoA-I induces cell death is through attenuating anti-stress activity of angiogenin. PMID: 24603325
Angiogenin was up-regulated in proliferative hemangiomas. Angiogenin serum levels correlate with the hemangioma stage. Angiogenin levels discriminate between proliferative hemangiomas and the control group and patients with venous malformations. PMID: 25068345
Depletion of cellular ANG expression abolished PLSCR1-enhanced rRNA transcription. PMID: 24356419
Human angiogenin has antimicrobial activity against C. albicans and S. pneumoniae. PMID: 12548285
Data show that the transcription of angiogenin (ANG) and ribonuclease 4 (RNASE4) promoter is influenced by RNA polymerase III (Pol III) elements and could be differentially regulated by an intragenic CCCTC binding factor (CTCF)-dependent chromatin loop. PMID: 24659782
Data shows ANG-stimulated rRNA transcription is not only an essential component for androgen-dependent growth of prostate cancer but also contributes to the transition of prostate cancer from androgen-dependent to castration-resistant growth status. PMID: 23851444
Nuclear ANG directly binds to the ANG-Binding Sequence within ERRgamma of ERRgamma gene and inhibits ERRgamma transcription to promote breast cancer cell proliferation. PMID: 23977052
These data suggest that one of the mechanisms by which ANG stimulates rRNA transcription is through an epigenetic activation of rDNA promoter. PMID: 24122807
Changes of angiogenin in the skin of patients with amyotrophic lateral sclerosis are related to the disease process. PMID: 23351638
Overexpression of ANG in pterygium body fibroblasts might be involved in active pterygium growth with thick pterygium body formation and increased risk of recurrence. PMID: 23989187
Our data do not support the association of angiogenin variants with Parkinson's disease in Han Chinese of mainland China. PMID: 23231972
Increased angiogenin may contribute to decreased T-cell zeta-chain expression in hemodialysis patients PMID: 23379493
fast molecular dynamics based method for determining the mechanisms of functional loss caused by mutations PMID: 23665167
Angiogenin is up-regulated in the hypoxic environment of oral squamous cell carcinoma PMID: 22694909
Dysregulated angiogenin expression may contribute to the pathogenesis of myositis as well as skin involvement via the vascular change in DM/CADM PMID: 23229115
analysis of Angiogenin in middle-aged type 1 diabetes patients PMID: 22940420
Angiogenin interacts with p53 and colocalizes in the nucleus. PMID: 22266868
In Alzheimer disease, serum angiogenin was decreased and cognitive function was associated with correlated with angiogenin levels. PMID: 22449478
serum angiogenin levels in cutaneous T cell lymphoma (CTCL)patients were significantly higher than healthy controls; angiogenin mRNA expression levels in lesional skin were elevated in erythrodermic CTCL compared to normal skin; results suggest enhanced angiogenin expression may be related with poor prognosis of erythrodermic CTCL PMID: 22526325

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