Recombinant Human ANXA2 Protein

Name: Recombinant Human ANXA2 Protein
Brand: Beta LifeScience
SKU: BL-1814NP
Availability: InStock

BL-1814NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Description	Recombinant Human Annexin A2 is produced by our E.coli expression system and the target gene encoding Ser2-Asp339 is expressed.
Accession	P07355
Synonym	Annexin A2; Annexin II; Annexin-2; Calpactin I Heavy Chain; Calpactin-1 Heavy Chain; Chromobindin-8; Lipocortin II; Placental Anticoagulant Protein IV; PAP-IV; Protein I; p36; ANXA2; ANX2; ANX2L4; CAL1H; LPC2D
Gene Background	Annexin A2 (ANXA2) is a member of the annexin family and has roles in the regulation of cellular growth and in signal transduction pathways. ANXA2 protein is associated with sickle cell osteonecrosis and the expression reduce of ANXA2 is associated with osteosarcoma metastases. ANXA2 functions as an autocrine factor, it can increases osteoclast formation and bone resorption. ANXA2 is involved in muscular dystrophies. In humans, the up-regulation of ANXA2 is related with colon adenocarcinoma cell differentiation.
Molecular Mass	38.6 KDa
Apmol Mass	35 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM Histidine-HCl, 6% Sucrose, 4% Mannitol, 0.05%Tween 80, pH 5.5.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9.
Subcellular Location	Secreted, extracellular space, extracellular matrix, basement membrane. Melanosome. Note=In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
Protein Families	Annexin family
Database References	HGNC: 537 OMIM: 151740 KEGG: hsa:302 STRING: 9606.ENSP00000346032 UniGene: Hs.511605

Gene Functions References

Mechanistic studies revealed that FOXD2AS1 upregulated the expression of the miR206 target gene ANXA2 in hepatocellular carcinoma by acting as a miR206 sponge. PMID: 30272362
study has localised AnxA2/S100A10 complexes to key anatomical locations in the placenta and suggests a role for this complex in amniotic epithelium, trophoblasts and syncytium, in addition to its well-known roles in endothelial cells PMID: 30143909
Inhibition of ANXA2 will affect the expression of downstream targets and downstream signaling pathways, leading to the suppression of the malignant progression of tumors. PMID: 30355917
our data suggest that overexpression of peroxiredoxin-2, annexin A2, and heat shock protein beta-1 was correlated with tumor invasion, metastasis, and poor prognosis, and therefore, these proteins may serve as potential diagnostic and therapeutic biomarkers. PMID: 29332450
aptamer wh6 can block MM cell adhesion to ANXA2 and block the proliferation of MM cells induced by ANXA2 PMID: 29906496
The results demonstrated that ANX2 and stromal tenascin C regulated invasion in addition to stemness and anoikis resistance, which are crucial for metastasis in the progression of pancreatic ductal adenocarcinoma. PMID: 29749431
highly expressed in gastric cancer, related to tumor size, histological differentiation, lymph node metastasis PMID: 29097873
models for annexin A2-mediated vesicle aggregation mechanisms. PMID: 29567212
Our profiling data provided a rich source for further study of the molecular mechanisms of acquired drug resistance inneuroblastoma ( NB). Further study may determine the role of ANXA2 as a prognostic biomarker and a potential therapeutic target for patients with multidrug-resistant NB. PMID: 28814318
These results suggested that increased ANXA2 and less expressed beta-catenin were correlated to adenomyosis-associated dysmenorrhea PMID: 28547742
These data suggested that Annexin A2 induces cisplatin resistance of non-small cell lung cancer (NSCLC)via regulation of JNK/c-Jun/p53 signaling, and provided an evidence that blockade of Annexin A2 could serve as a novel therapeutic approach for overcoming drug resistance in NSCLCs PMID: 28886730
Results show that Anxa2-Tyr23 phosphorylation is necessary for proliferation, invasion, and metastasis of breast cancer cells in vitro and in vivo. Tyr23-phosphorylated Anxa2 binds to STAT3 and serves as a key regulator of STAT3 signaling. PMID: 28470457
annexin A2 can potentially predict the development of pre-eclampsia. The low annexin A2 levels (<0.89 ng/ml) together with higher blood pressure and proteinuria constitute a higher risk to develop preeclampsia. PMID: 28501283
Variants in the Annexin A2 (ANXA2) gene directly influence plasma LDL-C levels. PMID: 28456096
this study showed that Annexin A2 inhibition suppresses proliferation and invasion in ovarian cancer via beta-catenin/EMT, proposing the potential role of Annexin A2 in the prevention and treatment of ovarian cancer PMID: 28440436
FNDC3B and ANXA2 expression correlate negatively with patient survival in hepatocellular carcinoma PMID: 27385217
UBAP2 formed a complex with Annexin A2 and promoted the degradation of Annexin A2 protein by ubiquitination, and then inhibited HCC progression. PMID: 27121050
results indicate that ANXA2 plays an important role to enhance the malignant behaviors of Hepatocellular carcinoma cells, and the enhancement is closely based on its remodeling to cell structures PMID: 27060670
Thus, tight regulation of AnxA2 function is an integral aspect of cellular homeostasis. The presence of AnxA2 in cancer cell-derived exosomes, as well as the potential regulation of exosomal AnxA2 by phosphorylation or other PTMs, are topics of great interes PMID: 28867585
Restoration of miR-101 expression reversed chemoresistance of drug-resistant gastric cancer cells by inhibiting viability and enhancing apoptosis via targeting ANXA2. PMID: 28609840
Annexin 2A (ANXA2) is over-expressed in Glioblastoma (GBM) and positively correlates with patient outcome. PMID: 27429043
Results confirm that both circRNA expression and even ANXA2 expression could be used as biomarkers of RR-MS with good values of specificity and sensitivity. PMID: 28651352
This study demonstrated that the expression level of AnxA2 was significantly higher in recurrent adamantinomatous craniopharyngioma compared with primary ones. AND AnxA2(+) adamantinomatous craniopharyngioma cells exhibited enhanced proliferation and migration ability compared with AnxA2(-) adamantinomatous craniopharyngioma cells. PMID: 27640198
Cercvical cancer stem cells express annexin II, an HPV co-receptor. PMID: 27008711
Annexin A2 (ANXA2) and prostate-specific antigen (PSA) proteins measured from biopsy tumour regions are unlikely to be good biomarkers for prediction of the clinical outcome of prostate cancer presenting with apparently localized disease. PMID: 29187477
anti-ANXA2 antibodies play a role in thrombotic mechanisms leading to recurrent pregnancy loss and placental vascular disease PMID: 27631133
results suggest that mycoplasma induces a resistance to multiple drugs in hepatocarcinoma cells which required the interaction of P37 and Annexin A2. The pathway downstream this interaction needs to be explored PMID: 28976984
Findings indicate that Munc13-4 supports acute WPB exocytosis by tethering WPBs to the plasma membrane via AnxA2-S100A10. PMID: 28450451
Detecting ANXA2 and ANXA4 expression may aid the evaluation of cervical carcinoma prognosis. PMID: 27402115
our studies show that this G-motif represents a novel and essential determinant for axonal localization of the Anxa2 mRNA mediated by the SMN complex. PMID: 28258160
The data data suggest that interactions between HIV-1 gp120 and A2 exist, though this interaction may be indirect. PMID: 27863502
Exosome-associated Annexin II plays an important role in angiogenesis and breast cancer metastasis. PMID: 27760843
ANXA2 stromal expression might play a key role in aggressive tumor phenotype associated with increased EMT CTCs release, however, other factors beyond ANXA2 are responsible for coagulation activation mediated by CTCs in breast cancer patients. PMID: 28476852
annexin A2 contributes to lung injury and fibrotic disease by mediating the fibrogenic actions of FXa. PMID: 28283478
Annexin A2 and HB-EGF are overexpressed and are being secreted into serum in Her-2 negative breast cancer patients. PMID: 27496793
results indicate that ANXA2 facilitates PS-ASO trafficking from early to late endosomes where it may also contribute to PS-ASO release PMID: 27378781
Annexins A2 and A8 in endothelial cell exocytosis and the control of vascular homeostasis PMID: 27451994
Rack1 regulated P-Glycoprotein activity, which was necessary for adriamycin-induced P-Glycoprotein - mediated phosphorylation of Anxa2 and Erk1/2. PMID: 27754360
The presence of vascular lesions in lupus nephritis (LN) appears to be associated with significant differences in the vascular expression of ANXA2. PMID: 26511441
expression elevated in lung squamous cell carcinoma tissue PMID: 28178129
annexin A2 but not follistatin is expressed in hepatocellular carcinoma PMID: 26189841
Our results place ANXA2 at the apex of a regulatory cascade that determines glioblastoma mesenchymal transformation and validate aSICS as a general methodology to uncover regulators of cancer subtypes. PMID: 27667176
Overexpression of ANXA2 is associated with colorectal cancer. PMID: 27468721
Annexin A2, up-regulated by IL-6, promotes ligament ossification in ankylosing spondylitis patients. PMID: 27697640
highly specific 30 kDa plant lectin could dissociate AnxA2 from endogenous lectin galectin-3 interaction at the cell surface. This dissociation could down-regulate Bcl-2 family proteins, cell proliferation, and migration simultaneously triggering cell apoptosis PMID: 26438086
Data show that ONECUT2, IGF2BP1, and ANXA2 proteins were confirmed to be microRNA-9 (miR-9) targets and aberrantly upregulated in hepatocellular carcinoma (HCC). PMID: 26547929
annexin A2 and S100A10 expressions are powerful predictors of serous ovarian cancer outcome. PMID: 26925708
expression of ANXA2 is associated with tumorigenesis and therapeutic resistance of nasopharyngeal carcinoma PMID: 26196246
Anxa2 binds to STAT3 and promotes epithelial to mesenchymal transition in breast cancer. PMID: 26307676
the role of Axna2 is instructive for understanding the development of RA, suppress the effect of Axna2 might provide a new potential measure for treatment of RA. PMID: 26963384

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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