Recombinant Human ASPRV1 Protein (N-10xHis & C-Myc)

Name: Recombinant Human ASPRV1 Protein (N-10xHis & C-Myc)
Brand: Beta LifeScience
SKU: BLC-11442P
Price: 349.0 USD
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: Recombinant proteins, Recombinant proteins holiday sale

Price Save $600

Size

100ug

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Product Overview

Description	This highly advanced Recombinant Human ASPRV1 protein can be used in the field of cell biology research. ASPRV1, also known as Retroviral-like aspartic protease 1, Skin-specific retroviral-like aspartic protease, or TPA-inducible aspartic proteinase-like protein, is a crucial component involved in various cellular processes. Our recombinant protein is produced using an innovative in vitro E.coli expression system, ensuring its exceptional quality and functionality. It encompasses the full length of the mature ASPRV1 protein, spanning amino acids 191 to 326, providing comprehensive insights into its biological functions and regulatory mechanisms. For ease of detection and purification, the Recombinant Human ASPRV1 protein is equipped with an N-terminal 10xHis tag and a C-terminal Myc tag. These tags enhance the solubility and simplifies the downstream applications of the protein. With a purity level of greater than 85% as determined by SDS-PAGE, you can have confidence in the reliability and consistency of this protein for your research needs. The Recombinant Human ASPRV1 is available in both liquid and lyophilized powder forms, providing flexibility in storage and experimental setups. Explore the vast potential of this protein in unraveling the intricate mechanisms of cellular biology and uncovering novel insights into cellular pathways and protein interactions.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	Q53RT3
Target Symbol	ASPRV1
Species	Human
Expression System	in vitro E.coli expression system
Tag	N-terminal 10xHis-tagged and C-terminal Myc-tagged
Target Protein Sequence	SMGKGYYLKGKIGKVPVRFLVDSGAQVSVVHPNLWEEVTDGDLDTLQPFENVVKVANGAEMKILGVWDTAVSLGKLKLKAQFLVANASAEEAIIGTDVLQDHNAILDFEHRTCTLKGKKFRLLPVGGSLEDEFDLE
Expression Range	191-326aa
Protein Length	Full Length of Mature Protein
Mol. Weight	19.9 kDa
Research Area	Cell Biology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Target Function	Protease responsible for filaggrin processing, essential for the maintenance of a proper epidermis organization.
Subcellular Location	Membrane; Single-pass membrane protein.
Database References	HGNC: 26321 OMIM: 611765 KEGG: hsa:151516 STRING: 9606.ENSP00000315383 UniGene: Hs.516253
Tissue Specificity	Expressed primarily in the granular layer of the epidermis and inner root sheath of hair follicles. In psoriatic skin, expressed throughout the stratum corneum. In ulcerated skin, expressed in the stratum granulosum of intact epidermis but almost absent f

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.