Recombinant Human Calprotectin (S100A8&S100A9) Protein

Name: Recombinant Human Calprotectin (S100A8&S100A9) Protein
Brand: Beta LifeScience
SKU: BLK-02605P-100UG
Availability: InStock

Human Calprotectin (S100A8&S100A9) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Collections: Recombinant proteins

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Product Overview

Description	Recombinant Human Calprotectin (S100A8&S100A9) Protein is expressed from E.coli with His tag at the C-Terminus.It contains Met1-Glu93(S100A8)&Thr2-Pro114(S100A9).
Purity	> 95% as determined by Tris-Bis PAGE
Accession	P05109(S100A8)&P06702(S100A9)
Target Symbol	Calprotectin (S100A8&S100A9)
Synonyms	Protein S100-A8；Calgranulin-A；CFAG；MRP-8；S100 calcium-binding protein A8；Protein S100-A9；MRP-14；S100 calcium-binding protein A9
Species	Human
Expression System	E. coli
Tag	C-His
Expression Range	Met1-Glu93(S100A8) & Thr2-Pro114(S100A9)
Mol. Weight	The protein has a predicted MW of 11.7 kDa(S100A8)&13.2 kDa(S100A9) same as Tris-Bis PAGE result.
Form	Lyophilized
Formulation	Lyophilized from 0.22um filtered solution in 2mM DTT, PBS (pH 7.2). Normally 8% trehalose is added as protectant before lyophilization.
Endotoxin	Less than 1EU per ug by the LAL method.
Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Shipping	Shipped at ambient temperature.
Gene Background	Calprotectin, a member of the widespread calcium-binding S-100 protein family, is present in remarkably high concentration in the cytoplasm of human neutrophils. The calprotectin molecule is composed of light (MRP8) and heavy (MRP14) subunits. Although not secreted from intact neutrophils, calprotectin release from dead and dying neutrophils creates high concentrations of the protein in inflammatory or abscess fluids and in the intestinal tract lumen of patients with inflammatory bowel disease.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.