Recombinant Human Caspase-5 (CASP5) Protein (His-SUMO)

Name: Recombinant Human Caspase-5 (CASP5) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-08622P
Price: 299.0 USD
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Price Save $99

Size

100ug

Product Overview

Description	Recombinant Human Caspase-5 (CASP5) Protein (His-SUMO) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P51878
Target Symbol	CASP5
Synonyms	Apoptosis related cysteine protease; CASP-5; CASP5; CASP5_HUMAN; Caspase-5 subunit p10; ICE(rel)-III; ICERELIII; ICH 3; ICH 3 protease; Protease ICH-3; Protease TY; TY; TY protease
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	DQKITSVKPLLQIEAGPPESAESTNILKLCPREEFLRLCKKNHDEIYPIKKREDRRRLALIICNTKFDHLPARNGAHYDIVGMKRLLQGLGYTVVDEKNLTARDMESVLRAFAARPEHKSSDSTFLVLMSHGILEGICGTAHKKKKPDVLLYDTIFQIFNNRNCLSLKDKPKVIIVQACRGEKHGELWVRD
Expression Range	137-327aa
Protein Length	Partial
Mol. Weight	37.8kDa
Research Area	Cell Biology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Thiol protease that acts as a mediator of programmed cell death. Initiates pyroptosis, a programmed lytic cell death pathway through cleavage of Gasdermin-D (GSDMD): cleavage releases the N-terminal gasdermin moiety (Gasdermin-D, N-terminal) that binds to membranes and forms pores, triggering pyroptosis. During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation.
Protein Families	Peptidase C14A family
Database References	HGNC: 1506 OMIM: 602665 KEGG: hsa:838 STRING: 9606.ENSP00000376849 UniGene: Hs.213327
Tissue Specificity	Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.

Gene Functions References

Long non-coding RNA CASP5 promotes the malignant phenotypes of human glioblastoma multiforme. PMID: 29715460
Study shows that CASP5 was positively correlated with inflammation in rheumatoid arthritis (RA), and provides evidence that certain CASP5 SNPs were associated with an increased risk of RA. PMID: 29158166
Th17 micro-milieu via IL-17A regulates NLRP1-dependent CASP5 activity in psoriatic skin autoinflammation. PMID: 28422993
CASP5 gene overexpression significantly promoted angiogenesis ability of HMEC-1 cells, which was probably achieved by inhibiting angpt-1/Tie2 and promoting VEGF-1 signal pathway. PMID: 26884849
The distribution of the other genotypes; rs507879, rs518604 and rs523104 of caspase-5) was not significantly different between patients with psoriasis vulgaris and the healthy controls. PMID: 25753570
Data show that exposure of hepatocytes to direct hypoxia resulted in acid sphingomyelinase activation and ceramide elevation associated with activation of caspase 5 and the subsequent cleavage of HuR and apoptotic cell death. PMID: 22906436
Mutual activation between caspase-5 and -1 suggests caspase-5 may work predominantly in concert with caspase-1 in modulating retinal pigment epithelium inflammatory responses. PMID: 21969293
analysis of concerted antigen processing of a short viral antigen by human caspase-5 and -10 PMID: 21454616
Caspase-5 and the inflammasome may have an important role in the inflammatory response in psoriasis. PMID: 21191419
Unfavorable significance of CASP5 heterozygous genotype explained by its role in inflammation-related processes. PMID: 20434535
Identification of caspase-5 coding region microsatellite mutations in vivo implicates this gene in the pathogenesis of human T-LBL/ALL PMID: 12479849
Caspase-5 might be a suppressor gene of highly metastatic potential in lung cancer PMID: 12964016
CASPASE-5, an acknowledged frameshift target in MSI+ gastrointestinal tract tumors, was frequently mutated in Microsatellite instability+ cell lines PMID: 15886296
novel exon was present in six alternatively spliced caspase-5 mRNA variants expressed in human peripheral blood mononuclear cells (PBMC) and encoded the previously unknown amino-terminus of caspase-5. PMID: 16893518
Caspase-5 gene is commonly mutated in the cancers with microsatellite instability suggesting that inactivation of caspase-5 may play a role in their tumorigenesis. PMID: 18430458
Coding polymorphisms in Casp5, Casp8 and DR4 genes may play a role in predisposition to lung cancer. PMID: 19203830
Single nucleotide polymorphisms in CASP5 and RBBP8 gene are associated with survival in ovarian cancer patients. PMID: 19270026

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.