Recombinant Human CCL20 Protein

Name: Recombinant Human CCL20 Protein
Brand: Beta LifeScience
SKU: BLK-02591P-100UG
Availability: InStock

Human CCL20 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Collections: Other recombinant proteins, Recombinant proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Description	Recombinant Human CCL20 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Ala27-Met96.
Purity	> 95% as determined by Tris-Bis PAGE
Accession	P78556
Target Symbol	CCL20
Synonyms	C-C motif chemokine 20; MIP-3-alpha; LARC; MIP3A; SCYA20; CKb4; Exodus; MIP-3a; ST38; exodus-1; MIP3 alpha
Species	Human
Expression System	E. coli
Tag	N-His
Expression Range	Ala27-Met96
Mol. Weight	The protein has a predicted MW of 9.3 kDa. The protein migrates to 12-14 kDa based on Tris-Bis PAGE result.
Form	Liquid
Formulation	Supplied as 0.22um filtered solution in PBS (pH 7.4).
Endotoxin	Less than 1EU per ug by the LAL method.
Storage	Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	Shipped with dry ice.
Gene Background	Recent evidence suggests that CC chemokine ligand 20 (CCL20) is upregulated after subarachnoid hemorrhage (SAH). CCL20 is upregulated in an SAH mouse model and in cultured primary microglia and neurons. CCL20-neutralizing antibody alleviated SAH-induced neurological deficits, decreased brain water content and neuronal apoptosis, and repressed microglial activation.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.