Recombinant Human chymase 1 / MCT1 Protein (His Tag)

Name: Recombinant Human chymase 1 / MCT1 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-1266
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	His
Host Species	Human
Accession	P23946
Synonym	chymase, CYH, MCT1
Background	The STR polymorphism in the CMA1 gene is associated with asthma and that this association is even stronger with atopic asthma. CMA1 and IL-4 in atopic asthma and for IL-4 in atopy in general. The local angiotensin II system (LAS) has numerous functions, including the regulation of growth and differentiation in the gastrointestinal tract. Angiotensin II (AngII) may be generated by angiotensin-I-converting enzyme (ACE) or mast cell chymase (CMA1) and plays an important role in inflammatory processes, although opinions differ as to which AngII-generating enzyme is primarily associated with AngII-mediated effects. ACE in the gastric mucosa and the microvasculature of granulation tissue may represent a novel therapeutic target for the promotion of healing processes in gastritis and ulceration using ACE inhibitors or AT1R antagonists. The gene for mast cell chymase (CMA1) is an ideal candidate for investigating genetic predisposition to atopic asthma, as it is an important mediator of inflammation and remodeling in the asthmatic lung. (CMA1) is important for the generation of angiotensin II and therefore might be associated with the pathogenesis of hypertension.
Description	A DNA sequence encoding the human CMA1 (P23946) (Met 1-Asn 247) was fused with a His tag at the C-terminus.
Source	Baculovirus-Insect Cells
Predicted N Terminal	Gly 20
AA Sequence	Met 1-Asn 247
Molecular Weight	The secreted recombinant human CMA1 consists of 238 a.a. and predicts a molecular mass of 26.6 kDa. It migrates as an approximately 33 kDa band in SDS-PAGE under reducing conditions.
Purity	>92% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
Subcellular Location	Secreted. Cytoplasmic granule. Note=Mast cell granules.
Protein Families	Peptidase S1 family, Granzyme subfamily
Database References	HGNC: 2097 OMIM: 118938 KEGG: hsa:1215 STRING: 9606.ENSP00000250378 UniGene: Hs.135626
Tissue Specificity	Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions.

Gene Functions References

These findings reveal the critical role of hypoxia in producing local angiotensin II by a lactate-chymase-dependent mechanism and highlight the importance of local angiotensin II in regulating radioresistance of hypoxic tumor cells. PMID: 28205588
Cleavage of the alarmins by Human mast cell chymase and human neutrophil cathepsin G suggests a function in regulating excessive inflammation. PMID: 28053237
activation of endothelial CLP/chymase may directly relate to the increased inflammatory phenotypic changes in the vascular system in women with preeclampsia PMID: 19494363
Placenta-derived chymotrypsin-like protease contributes to the altered endothelial barrier function in preeclampsia. PMID: 19126871
promoter single nucleotide polymorphism is not associated with Dengue Hemorrhagic Fever and Dengue Shock Syndrome in Vietnam and Philippines PMID: 25797204
Our data demonstrated that mast cell chymase plays an important role in keloid formation through TGF-beta1/Smad signaling pathway. PMID: 25120737
These data suggest a possible contribution of human chymase activation to LPS-induced mortality PMID: 24516344
we present data indicating that MC tryptase and chymase contribute to the development of OSF and malignant transformation of the overlying epithelium. PMID: 24874976
The polymorphisms and haplotypes of the CMA1 locus are associated with cardiac hypertrophy in male patients with symptomatic aortic stenosis. PMID: 24823657
CMA1 gene single nucleotide polymorphisms is associated with essential hypertension. PMID: 25119908
Significant association between the AG genotype of CMA1 A polymorphism and Angina Pectoris and Ventricular Extrasystoles was observed. PMID: 25102745
The accumulated data in this review suggest that mast cells, their tryptases, and their chymases play important roles in tissue repair. PMID: 24507159
demonstrate the generation of Pso p27 from SCCA1 with extracts from psoriatic scale and even more remarkably, the generation of Pso p27 from SCCA1 in the presence of mast cell associated chymase PMID: 24560885
The present results indicated PRCP rs7104980 can be considered as a marker for EH and Hap3 GAGCACTAACA (PRCP) and Hap16 TTTA (CMA1) might be associated with essential hypertension in Chinese Han population. PMID: 22679278
Purified chymase mixed with fragmented heparin derived from heparanase-expressing cells show greater release from collagen gels than the enzyme alone or mixed with macromolecular heparin derived from mock cells. PMID: 24344642
Data indicate that the best Fynomer (the binding proteins derived from the Fyn SH3 domain) was found to bind chymase with a KD of 0.9 nM and koff of 6.6x10 (-4) s (-1) , and to selectively inhibit chymase activity with an IC 50 value of 2 nM. PMID: 22653218
Both IgE and chymase associate with diabetes status. PMID: 22194960
As mast cells are an important source of VEGF, tryptase, and chymase, these findings suggest that mast cell activation and mast cell-derived mediators participate in the development of Dengue hemorrhagic fever. PMID: 22363824
Chymase rs1800875 polymorphism is associated with the progression of immunoglobulin A (IgA) nephropathy in Korean patients. PMID: 21150220
a dominant role of cardiac chymase in the formation of Ang II from Ang-(1-12) PMID: 22180785
Two different populations of mast cells were found in melanocytic skin; one expressing both chymase and tryptase and the other with tryptase only. PMID: 22102069
mast cell-derived protein is present in uterine cervical carcinoma PMID: 21274549
Polymorphism rs1800875 of CMA1 may be associated with serum IgE level in coronary heart disease subjects, but not with chymase level in normal coronary intimae PMID: 21796807
human chymase contributes to blood glucose levels and mortality during the progression of diabetes PMID: 21786536
short tandem repeat genetic polymorphism is associated with bronchial asthma in a Swiss cohort study PMID: 20736038
Elevated maternal chymase activity and enhanced protease immunostaining in the maternal vessel endothelium may constitute the exacerbated inflammatory state and account for the increased vascular Ang II sensitivity in preeclampsia. PMID: 20670150
Positions 143 (Arg) and 192 (Lys) in human mast cell chymase contribute to the strong preference for negatively charged amino acids at substrate position P2'. PMID: 20423454
The levels of tryptase and chymase expression are greatly increased in human lung tissue of anaphylactic shock. PMID: 19697770
The S1 primary specificity pocket defines substrate specificity of chymases. PMID: 11852067
The mast cell chymase A3255 allele was shown to have an effect on HDL cholesterol metabolism. PMID: 12047032
The local release of mast cell chymase has potentially profound effects on airway smooth muscle cell function by disruption of the cell-associated matrix and inhibition of epidermal growth factor-induced smooth muscle cell proliferation. PMID: 12097409
Results suggest the additive effect of angiotensin I-converting enzyme (ACE) and heart chymase (CMA) gene polymorphisms on the increase in left ventricular mass in NIDDM patients. PMID: 12165749
Chymase may play a role in heart remodeling by increasing Ang II formation and activating MMP-9, and the regulation of collagen I gene expression. PMID: 12359984
Both the ACE and chymase-like enzyme activities in the aneurysmal aortae were significantly higher than those in the control aortae. PMID: 12484503
Degradation of phospholipid transfer protein (PLTP) and PLTP-generated pre-beta-high density lipoprotein by this enzyme in mast cells impairs high affinity efflux of cholesterol from macrophage foam cells. PMID: 12531890
Interactions among the loops bordering and defining the active site appear to influence both the zymogen and the activated conformations of chymase in this model. PMID: 12614156
albumin is a substrate of human chymase PMID: 12815038
new class of chymase inhibitor through a substituent analysis of MWP00965 chemically synthesized PMID: 14592513
chymase depletes pre-beta-high density lipoprotein, which impairs ATP-binding cassette transporter A1- but not scavenger receptor class B type I-mediated lipid efflux to high density lipoprotein PMID: 14701812
MCT1 immunoreactivity was visible in blood vessel walls as early as the 13th week of gestation mainly in the visual cortical plate and subplate. PMID: 14757520
mast cell chymase-1 is unlikely to influence blood pressure levels in the Japanese population. PMID: 15106801
Bladder fibrosis may be mediated by mast cell chymase stimulation of collagen synthesis. PMID: 15227657
The synthesis of new potential inhibitors of human chymase is described PMID: 15449728
Tryptase and chymase and protein levels were determined in mast cells in fibrosarcoma. PMID: 15638376
mast cell chymase activates ERK and p38 probably through G-protein-coupled receptor, and the ERK but not p38 cascade may have a crucial role in chymase-induced migration of eosinophils PMID: 15919053
A novel (TG)n(GA)m repeat polymorphism 254 bp downstream of the mast cell chymase (CMA1) gene is associated with atopic asthma and total serum IgE levels. PMID: 15924217
Chymase-induced apoptosis of conjunctival epithelial cells represents anoikis, which is a slowly occurring apoptotic process induced by lack of adhesion to an extracellular matrix. PMID: 16020275
Significant association between the CMA1 promoter polymorphism rs1800875 and atopic eczema supports the hypothesis that CMA1 serves as candidate gene for atopic eczema. PMID: 16134991
activated human skin mast cells (MCs) convert CTAP-III into biologically active NAP-2 through proteolytic cleavage by released chymase. PMID: 16317101
The CMA1 polymorphisms studied do not contribute to disease susceptibility in Japanese or Dutch sarcoidosis patients. PMID: 16446531

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