Recombinant Human DCXR Protein (His Tag)

Name: Recombinant Human DCXR Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-1563
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	His
Host Species	Human
Accession	Q7Z4W1
Synonym	DCR, HCR2, HCRII, KIDCR, P34H, PNTSU, SDR20C1, XR
Background	DCXR, also known as HCR2, belongs to the short-chain dehydrogenases/reductases (SDR) family. It is highly expressed in kidney, liver and epididymis. In the epididymis, DCXR is mainly expressed in the proximal and distal sections of the corpus region. HCR2 is weakly or not expressed in brain, lung, heart, spleen and testis. DCXR catalyzes the NADPH-dependent reduction of several pentoses, tetroses, trioses, alpha-dicarbonyl compounds and L-xylulose. DCXR participates in the uronate cycle of glucose metabolism. It may play a role in the water absorption and cellular osmoregulation in the proximal renal tubules by producing xylitol, an osmolyte, thereby preventing osmolytic stress from occurring in the renal tubules.
Description	A DNA sequence encoding the mature form of human DCXR (Q7Z4W1) (Met1-Cys244) was expressed with a His tag at the N-terminus.
Source	E.coli
Predicted N Terminal	His
AA Sequence	Met1-Cys244
Molecular Weight	The recombinant human DCXR consists of 259 a.a. and predicts a molecular mass of 27.8 KDa. It migrates as an approximately 29 KDa band in SDS-PAGE under reducing conditions.
Purity	>95% as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile PBS, 20% Glycerol, pH 7.4..
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Catalyzes the NADPH-dependent reduction of several pentoses, tetroses, trioses, alpha-dicarbonyl compounds and L-xylulose. Participates in the uronate cycle of glucose metabolism. May play a role in the water absorption and cellular osmoregulation in the proximal renal tubules by producing xylitol, an osmolyte, thereby preventing osmolytic stress from occurring in the renal tubules.
Subcellular Location	Membrane; Peripheral membrane protein.
Protein Families	Short-chain dehydrogenases/reductases (SDR) family
Database References	HGNC: 18985 OMIM: 260800 KEGG: hsa:51181 STRING: 9606.ENSP00000303356 UniGene: Hs.9857
Associated Diseases	Pentosuria (PNTSU)
Tissue Specificity	Highly expressed in kidney, liver and epididymis. In the epididymis, it is mainly expressed in the proximal and distal sections of the corpus region. Weakly or not expressed in brain, lung, heart, spleen and testis.

Gene Functions References

Ureaplasma urealyticum infection may affect the level of P34H protein expression on spermatozoa PMID: 28012250
identification of DCXR as an enzyme activity mediating chemical redox cycling suggests that it may be important in generating cytotoxic reactive oxygen species in the lung PMID: 28595002
Low expression of DCXR is associated with hepatocellular carcinoma. PMID: 27658779
EGCG is a potential inhibitor to human DCXR. PMID: 23661708
combined frequency of the two mutant DCXR alleles in 1,067 Ashkenazi Jewish controls was 0.0173, suggesting a pentosuria frequency of approximately one in 3,300 in this population PMID: 22042873
highly expressed in kidney and liver PMID: 11882650
crystallization and preliminary crystallographic analysis PMID: 12136162
structure of the tetrameric form of human L-Xylulose reductase PMID: 15906319
Our results demonstrate that the proportion of positive P34H cases that produced embryos in vitro clearly differs from cases with undetectable levels of P34H (P<.001). PMID: 16616743
These results suggested that altered HCR2 expression might play roles in the carcinogenesis and progression of hepatocellular carcinoma. PMID: 16847567
A significant proportion of men investigated for male infertility may be epididymal protein P34H deficient. PMID: 17434498
Decreased membranous expression of DCXR with altered subcellular localization appears to be associated with malignant progression of melanocytic lesions in human skin. PMID: 17576332
DCXR trranfected into mice may function in the removal of renal alpha-dicarbonyl compounds under oxidative circumstances, but it is not sufficient to suppress acute renal fibrosis. PMID: 18079483
DCXR overexpression has the potential to be an additional useful biomarker for prostate cancer. PMID: 18086765
The action of human XR may be regulated by cellular redox conditions through reversible disulfide-bond formation and by S-cysteinylation. PMID: 19337691

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.