Recombinant Human DLL3 (27-215) Protein, Active

Name: Recombinant Human DLL3 (27-215) Protein, Active
Brand: Beta LifeScience
SKU: BLK-02268P-100UG
Availability: InStock

Human DLL3 (27-215) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Collections: Recombinant proteins

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Product Overview

Description	Recombinant Human DLL3 (27-215) Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Ala27-Glu215.
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Accession	Q9NYJ7
Target Symbol	DLL3 Domain
Synonyms	Delta3; DLL3; Pudgy; SCDO1; SCDO1delta3
Species	Human
Expression System	HEK293
Tag	N-His-Flag
Expression Range	Ala27-Glu215
Mol. Weight	The protein has a predicted MW of 22.30 kDa. Due to glycosylation, the protein migrates to 24-25 kDa based on Tris-Bis PAGE result.
Form	Lyophilized
Formulation	Lyophilized from 0.22 um filtered solution in PBS, 360mM NaCl, 200mM L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Endotoxin	Less than 1EU per ug by the LAL method.
Activity	Immobilized Human DLL3 (27-215) , His Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA. Contact us for detailed testing images.
Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Shipping	Shipped at ambient temperature.
Gene Background	Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. DLL3 inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity).

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.