Recombinant Human ENPP1 Protein

Name: Recombinant Human ENPP1 Protein
Brand: Beta LifeScience
SKU: BL-9806PSL
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	His
Host Species	Human
Accession	P22413
Synonym	Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, E-NPP 1, Membrane component chromosome 6 surface marker 1, Phosphodiesterase I/nucleotide pyrophosphatase 1, Plasma-cell membrane glycoprotein PC-1, ENPP1, M6S1, NPPS, PC1, PDNP1, NPP1, PC-1, PCA1, ARHR2, COLED.
Background	ENPP1 (Ectonucleotide Pyrophosphatase/Phosphodiesterase 1) is a Protein Coding gene. Diseases associated with ENPP1 include Cole Disease and Hypophosphatemic Rickets, Autosomal Recessive, 2. Among its related pathways are NAD metabolism and Riboflavin metabolism. Gene Ontology (GO) annotations related to this gene include nucleic acid binding and protein homodimerization activity. An important paralog of this gene is ENPP3.
Description	ENPP1 Human Recombinant was expressed in mouse myeloma cell line and fused to a 6 a.a His tag at N-Terminus. The Human ENPP1 is purified by unique purification methods.
Source	Mouse myeloma cell line, NS0-derived.
Molecular Weight	96 kDa. 100-135 kDa, reducing conditions
Purity	>95.0% as determined by SDS-PAGE.
Endotoxin	<1.0 EU per μg ENPP1 protein by the LAL method.
Bioactivity	Measured by its ability to hydrolyze thymidine 5'-monophosphate p-nitrophenyl ester. The specific activity is >35,000 pmol/min/μg, as measured under the described conditions.
Formulation	Recombinant Human ENPP1 Protein was supplied as a 0.2 μm filtered solution in Tris and NaCl.
Stability	Recombinant ENPP1 protein is stable for 6 months at -70°C
Usage	For Research Use Only
Storage	Store human ENPP1 protein at -20--70°C.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.