Recombinant Human Factor VII Protein (His Tag)

Name: Recombinant Human Factor VII Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-1965
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	His
Host Species	Human
Accession	P08709
Synonym	Coagulation factor 7, Coagulation factor VII, SPCA
Background	Coagulation factor VII, also known as Serum prothrombin conversion accelerator, Factor VII, F7 and FVII, is a member of the peptidase S1 family. Factor VII is one of the central proteins in the coagulation cascade. It is an enzyme of the serine protease class, and Factor VII (FVII) deficiency is the most frequent among rare congenital bleeding disorders. Factor VII contains two EGF-like domains, one Gla (gamma-carboxy-glutamate) domain and one peptidase S1 domain. The main role of factor VII is to initiate the process of coagulation in conjunction with tissue factor (TF). Tissue factor is found on the outside of blood vessels, normally not exposed to the blood stream. The action of the Factor VII is impeded by tissue factor pathway inhibitor (TFPI), which is released almost immediately after initiation of coagulation. Factor VII is vitamin K dependent and is produced in the liver. Upon vessel injury, tissue factor is exposed to the blood and circulating Factor VII. Once bound to TF, FVII is activated to FVIIa by different proteases, among which are thrombin (factor IIa), factor Xa, IXa, XIIa, and the FVIIa-TF complex itself. Recombinant activated factor VII (rFVIIa) is a haemostatic agent, which was originally developed for the treatment of haemophilia patients with inhibitors against factor FVIII or FIX. FVIIa binds specifically to endothelial protein C receptor (EPCR), a known cellular receptor for protein C and activated protein C, on the endothelium. rFVIIa is a novel hemostatic agent, originally developed for the treatment of hemorrhage in hemophiliacs with inhibitors, which has been successfully used recently in an increasing number of nonhemophilic bleeding conditions.
Description	A DNA sequence encoding the human F7 isoform B (P08709-2) (Met 1-Pro 444) was expressed, fused with a His tag at the C-terminus.
Source	HEK293
Predicted N Terminal	Ala 39
AA Sequence	Met 1-Pro 444
Molecular Weight	The secreted mature form of recombinant human F7 consists of 417 a.a. and predictes a molecular mass of 46.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh F7 is approximately 50 kDa.
Purity	>95% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
Subcellular Location	Secreted.
Protein Families	Peptidase S1 family
Database References	HGNC: 3544 OMIM: 227500 KEGG: hsa:2155 STRING: 9606.ENSP00000364731 UniGene: Hs.36989
Associated Diseases	Factor VII deficiency (FA7D)
Tissue Specificity	Plasma.

Gene Functions References

TF-FVIIa/trypsin-mediated PAR2 activation leads to enhanced MMP-2 expression in human breast cancer cells contributing to tumor progression. PMID: 29870887
The current meta-analysis suggested that polymorphism of R353Q in factor VII was not associated with the MI risk. PMID: 30278561
The obtained results suggest a possible protective role of Gln353 and -122C alleles in Recurrent Miscarriage. PMID: 27504943
FVIIa-antithrombin levels in early and late preeclampsia PMID: 28887028
model predicts that small vesicles promote activation of FX by the extrinsic tenase (VIIa/TF) significantly better than large vesicles PMID: 28935233
Low levels of FVII:C and FVIIa reflected the degree of consumption of the coagulation factor among paediatric sepsis patients with disseminated intravascular coagulation. PMID: 28492702
Report a good correlation between the type of F7 mutation and/or polymorphisms and FVII:C levels, without a direct link between FVII:C and bleeding tendency in factor VII deficiencies. PMID: 28447100
Polymorphism rs6046 of the FVII gene is associated with the development of fetal growth retardation in Central Russia. PMID: 28544373
A common pathogenic mechanism, possibly a defective folding of the mutant proteins, was triggered by the FVII mutations. The misfolded state led to impaired trafficking of these proteins causing Endoplasmic reticulum retention, which would explain the low to very low FVII plasma levels observed in patients carrying these mutations. PMID: 29246447
FVIIa-antithrombin but not FVIIa is a ligand for LRP1, and LRP1 contributes to the clearance of FVIIa-antithrombin in vivo PMID: 27614059
heterozygotes for FVII deficiency show rare bleeding manifestations which are also present in the unaffected family members with normal FVII levels. This indicates that Factor VII activity levels played no role in the occurrence of the bleeding symptoms. Furthermore, FVII levels of around 0.40 IU/dl are capable of assuring a normal hemostasis. PMID: 28176610
plasma FVIIa-AT has a thrombophilic role in total and cardiovascular mortality risk in patients with clinically stable stable coronary artery PMID: 27061056
Family-based association study revealed that the G allele of Protein Z rs2273971, and haplotypes GA, CG, and CGA of Protein Z and factor VII had a significant effect on cerebral hemorrhage susceptibility. PMID: 27350683
Our study findings suggest a link between FVII and AR in prostate cancer pathogenesis. PMID: 27434295
Suggest that the hemostatic effect of pharmacological doses of rFVIIa in antibody-induced hemophilia mice stems from a TF-independent mechanism PMID: 26727350
Large deletions play a minor but essential role in the mutational spectrum of the F7 and F10 genes. Copy number analyses (e. g. MLPA) should be considered if sequencing cannot clarify the underlying reason of an observed coagulopathy. Of note, in cases of combined FVII/FX deficiency, a deletion of the two contiguous genes might be part of a larger chromosomal rearrangement. PMID: 26540129
Holders of the R allele had significantly higher activity of coagulation factor F7 (97.66 +/- 15.48 against 83.37 +/- 15.16, p = 0.002) and factor F2 (107.45 +/- 6.03 against 103.75 +/- 6.81, p = 0.023) than holders of the Q allele PMID: 27215039
The aim of the study was to evaluate the molecular basis behind low levels of FVII activity (FVII:C) levels in a cohort of Brazilian patients. PMID: 25828579
Decreased plasma levels of FVIIa in patients with deep vein thrombosis may indicate ongoing consumption of FVIIa and suggest a contributory role for TF in venous thrombus formation. PMID: 25891834
The story of FVII well summarizes the efforts of both theoretical and clinical approaches in the characterization of a coagulation disorder, that is, among the rare bleeding conditions, most frequently encountered in clinical practice. PMID: 25973586
Identified are the FVII gene mutations in the Chinese Han population of four unrelated FVII-deficient patients, and the effect of these mutations on the function of FVII molecule level has also been elucidated. PMID: 25767893
Letter: large volume of distribution of rFVIIa explains the persistence of some clotting potential when FVII:C is no longer detectable in plasma of patients with inherited FVII deficiency. PMID: 24763923
Structural differences in the carboxyl-terminus between the inherited FVII and the therapeutic molecules contributed to the immune response. A naturally-occurring, poorly secreted and 5-residue truncated FVII (FVII-462X) escaped inhibition. PMID: 25104096
Polymorphism R353Q (coagulation factor VII) does not represent a protective or risk factor for acute myocardial infarction in young Mexican individuals PMID: 25393858
Two heterozygous mutations of F7, g.11349G>A and g.11482T>G, is associated with hereditary coagulation factor deficiency. PMID: 25863091
factor VIIa improved heat intolerance by attenuating hypothalamic neuronal apoptosis and damage. PMID: 25033928
Results show that the conformational allosteric activation signal extends to the EGF1 domain in the light chain of factor VIIa (FVIIa). PMID: 25344622
F7-323Ins10 was associated with lower factor VII levels, but not with individual intraventricular hemorrhage risk in preterm infants. PMID: 25179312
Decanucleotide insertion polymorphism of factor VII significantly influences the risk of thrombosis in patients with essential thrombocythemia. PMID: 24617727
High Coagulation factor VII expression is associated with breast cancer. PMID: 25447311
Persistently high levels of factor VII is associated with insulin resisitance. PMID: 24344794
The variability in Factor VII throughout the menstrual cycle in premenopausal women is no greater than for postmenopausal women or men. PMID: 24382103
The obtained results suggest a probable protective role of -323P10 allele against the risk of miscarriage in women with > or = 3 recurrent pregnancy losses. PMID: 25219139
plasma level is associated with ischemic stroke subtypes PMID: 24048512
Results suggest no association between R353Q polymorphism for factor VII and the presence or progression of coronary artery disease in the Iranian population. PMID: 24469878
Identification of a homozygous mutation in exon 8 of coagulation FVII that is responsible for factor VII deficiency in a Chinese pedigree. PMID: 23672839
Eight missense mutations were identified on the Factor 7 gene (p.Cys82Tyr, p.Cys322Ser, p.Leu357Phe, p.Thr410Ala, c-57C>T) PMID: 23731332
Data indicate that nanobilayers containing phosphatidic acid (PA) bound substantially more of two proteins, factor VIIa and activated protein C, than did equivalent bilayers containing phosphatidylserine (PS). PMID: 23879866
Data indicate that the interlaboratory precision was better for normal specimens than for factor VII (FVII) <20 U/dL with a mean coefficient of variation (CV) of 17.2% per specimen. PMID: 23590660
Polymorphisms in the coagulation factor VII gene modulate the susceptibility to coronary artery disease in Tunisian Arabs. PMID: 22932775
Rab GTPases regulate endothelial cell protein C receptor-mediated endocytosis and trafficking of factor VIIa. PMID: 23555015
elevated FVII levels, and the -323P0/10 but not R353Q polymorphism, constitute risk factors for ACS. PMID: 23275237
Data indicate that hfVII-LC and hIgG1-Fc can effectively inhibit tumor growth and metastases in SCID mice with tissue factor (TF) over-expressing colon cancer. PMID: 23494077
Data suggest that plasma FVIIa-AT complex (coagulation factor VII-antithrombin III) is higher in portal vein thrombosis (PVT; without cirrhosis) than in healthy subjects; no difference in FVIIa-AT complex is observed in cirrhosis with/without PVT. PMID: 22958499
Report comprehensive molecular analysis of FVII deficiency affected patients in North Tunisia. PMID: 22873696
Prothromin genetic mutatation is one of the risk factor in the development of venous thromboembolism and myocardial infarction. PMID: 23382263
glucose deprivation enhanced F7 expression in a mechanism reliant on prior ATF4 upregulation primarily due to increased transcription from the ATF4 gene. PMID: 22848420
rs6046A allele in F7 associated with decreased blood pressure levels (P PMID: 22815813
Intracellular depletion of GTP results in upregulation of coagulation factor VII. PMID: 23050902
functional analysis of lethal factor VII deficiency due to novel mutations in the F7 promoter reveals disruption of HNF4 binding site PMID: 22628013

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