Recombinant Human FAM3C Protein (Fc Tag)

Name: Recombinant Human FAM3C Protein (Fc Tag)
Brand: Beta LifeScience
SKU: BLPSN-1978
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	Fc
Host Species	Human
Accession	Q92520
Synonym	GS3786, ILEI
Background	FAM3C, also known as ILEI, is a member of theFAM3 family. It can be detected in resent in most secretory epithelia. FAM3C may be involved in retinal laminar formation. It promotes epithelial to mesenchymal transition. A change in expression of FAM3C has been noted in pancreatic cancer-derived cells. ILEI is overexpressed and/or altered in intracellular localization in multiple human tumors, an event strongly correlated to invasion/EMT, metastasis formation, and survival in human colon and breast cancer.
Description	A DNA sequence encoding the human FAM3C (Q92520)(Met1-Asp227) was expressed with the Fc region of human IgG1 at the C-terminus.
Source	HEK293
Predicted N Terminal	Gln 25
AA Sequence	Met1-Asp227
Molecular Weight	The recombinant human FAM3C/Fc is a disulfide-linked homodimer. The reduced monomer comprises 444 a.a. and has a predicted molecular mass of 49.2 kDa. The apparent molecular mass of the protein is approximately 53 and 35 kDa in SDS-PAGE under reducing conditions.
Purity	(73.6+19.1)% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile PBS, pH 7.4..
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	May be involved in retinal laminar formation. Promotes epithelial to mesenchymal transition.
Subcellular Location	Secreted. Cytoplasmic vesicle. Note=Cytoplasmic in some cancer cells.
Protein Families	FAM3 family
Database References	HGNC: 18664 OMIM: 608618 KEGG: hsa:10447 STRING: 9606.ENSP00000353025 UniGene: Hs.272375
Tissue Specificity	Present in most secretory epithelia (at protein level).

Gene Functions References

The ILEI appears to play a crucial role in mediating TGF-beta1-induced EMT through the Akt and ERK pathways, which may provide a therapeutic target for the treatment of fibrotic kidney diseases. PMID: 29336056
Covalent dimerization of ILEI is associated with breast cancer metastasis. PMID: 28837266
Data suggest that ILEI does not form interleukin-like four helix-bundle structures; ILEI exists as monomers and as covalent dimers; ILEI dimer exhibits a trans-linked domain swap converting an intramolecular disulfide to an intermolecular disulfide; dimeric ILEI appears to be involved in neoplastic invasiveness. PMID: 28751379
Results demonstrate that phenotype switching regulates ILEI expression, and that ILEI regulates partial phenotype switching in MITF-low melanoma cell lines. PMID: 28545079
Ectopic overexpression of UBE4A, but not UBE3C, in cells was downregulated in vitro migration and invasion in these cells. Cumulatively, our data reveals a novel post-translational regulatory mechanism of regulating ILEI1 expression, a protein required for metastatic progression in prostate cancer cells PMID: 27862841
Study used immunohistochemical staining and biochemical fractionation to examine the regional distribution and subcellular localization of ILEI in mouse brains and in autopsy brains of patients with Alzheimer's disease; results suggest that a decline of ILEI expression may cause accumulation of Abeta in the brain and the eventual development of Alzheimer's disease. PMID: 27256505
FAM3C expression was dramatically increased in esophageal squamous cell carcinoma and might serve as a valuable prognostic indicator for esophageal squamous cell carcinoma patients after surgery PMID: 26498278
Altered subcellular ILEI localization strongly correlates with high tumor cell-associated uPAR protein expression. PMID: 25212966
Our findings indicate that cytoplasmic ILEI expression is a potential marker of EMT and tumour progression in colorectal cancer. ILEI is an independent predictive factor associated with poor prognosis in colorectal cancer. PMID: 24738665
ILEI could induce, as well as maintain, CD24(low)CD44(high) subpopulation in A549 cells treated with TGF-beta, which might explain its capability to induce metastatic progression. PMID: 24072492
At the FAM3C gene locus where rs7776725 was located, we identified several other SNPs (rs4727922, rs1803389, rs718766 and rs7793554) that were also associated with BMD. PMID: 22159821
A cytokine-like protein found in almost all tissues. PMID: 12160727
ILEI is overexpressed and/or altered in intracellular localization in multiple human tumors. PMID: 16959614
Data show that FAM3C is decreased in the CSF of temporal lobe epilepsy patients. PMID: 19109932

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.