Recombinant Human Fructose-Bisphosphate Aldolase A (ALDOA) Protein (His)

Name: Recombinant Human Fructose-Bisphosphate Aldolase A (ALDOA) Protein (His)
Brand: Beta LifeScience
SKU: BLC-01342P
Price: 349.0 USD
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Size

100ug

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Product Overview

Description	Recombinant Human Fructose-Bisphosphate Aldolase A (ALDOA) Protein (His) is produced by our Baculovirus expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P04075
Target Symbol	ALDOA
Synonyms	Lung cancer antigen NY-LU-1Muscle-type aldolase
Species	Homo sapiens (Human)
Expression System	Baculovirus
Tag	C-6His
Target Protein Sequence	PYQYPALTPEQKKELSDIAHRIVAPGKGILAADESTGSIAKRLQSIGTENTEENRRFYRQLLLTADDRVNPCIGGVILFHETLYQKADDGRPFPQVIKSKGGVVGIKVDKGVVPLAGTNGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSALAIMENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHIYLEGTLLKPNMVTPGHACTQKFSHEEIAMATVTALRRTVPPAVTGITFLSGGQSEEEASINLNAINKCPLLKPWALTFSYGRALQASALKAWGGKKENLKAAQEEYVKRALANSLACQGKYTPSGQAGAAASESLFVSNHAY
Expression Range	2-364aa
Protein Length	Full Length of Mature Protein
Mol. Weight	40.4 kDa
Research Area	Metabolism
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.
Subcellular Location	Cytoplasm, myofibril, sarcomere, I band. Cytoplasm, myofibril, sarcomere, M line.
Protein Families	Class I fructose-bisphosphate aldolase family
Database References	HGNC: 414 OMIM: 103850 KEGG: hsa:226 STRING: 9606.ENSP00000336927 UniGene: Hs.513490
Associated Diseases	Glycogen storage disease 12 (GSD12)

Gene Functions References

Results showed that ALDOA was upregulated in renal cell carcinoma (RCC) samples and cell lines and significantly associated with metastasis and survival. Overall, data revealed that ALDOA functions as a tumor promoter, plays a prominent role in proliferation, migration, and invasion of RCC cells with high expression, and may promote EMT and activate the Wnt/betacatenin signaling pathway. PMID: 29693182
The results indicate that ALDOA and PGK1 may indicate resistance to cisplatin in osteosarcoma PMID: 29199648
Knockdown of ALDOA in QBC939 and RBE cells attenuated the cell proliferation and induced a higher apoptosis rate. PMID: 30121648
Silencing aldolase A suppressed colon cancer cell proliferation and invasion and inhibited the epithelial-mesenchymal transition phenotype. Aldolase A protein expression in colon cancer was related to tumor location, tumor clinical stage and survival. PMID: 29453983
mir-122 and its targets G6PC3, ALDOA and CS play roles in the hypoxia responses that regulate glucose and energy metabolism and can serve as hypoxia biomarkers. PMID: 27793029
Aldolase A promotes lung cancer metastasis via PHD-mediated stabilization of HIF-1alpha and the subsequent activation of MMP9. PMID: 28610954
Findings show that ALDOA expression is up-regulated in colorectal cancer (CRC) and is a hypoxia-inducible prognostic factor that is closely related to CRC malignancy. PMID: 28000858
Overexpression of ALDOA is associated with colorectal cancer. PMID: 27468721
In vitro and in vivo results demonstrated that ALDOA was associated with proliferation and metastasis of pancreatic cancer cells. PMID: 26854714
Study shows that PI3K directly coordinates glycolysis with cytoskeletal dynamics in an AKT-independent manner. Growth factors or insulin stimulate the PI3K-dependent activation of Rac, leading to disruption of the actin cytoskeleton, release of filamentous actin-bound aldolase A, and an increase in aldolase activity. PMID: 26824656
Our results expand the clinical spectrum of aldolase A deficiency to isolated temperature-dependent rhabdomyolysis, and suggest that thermolability may be tissue specific. We also propose a treatment for this severe disease PMID: 25392908
Study provides evidence supporting a critical functional role of ALDOA in osteosarcoma progression, metastasis and perhaps chemoresistance. PMID: 25215901
ALDOC, Aldolase A (ALDOA) and Aldolase B (ALDOB) activate Wnt signaling. PMID: 24993527
ALDOA is highly expressed in lung squamous cell carcinoma (LSCC) and its expression level is correlated with LSCC metastasis, grades, differentiation status and poor prognosis. PMID: 24465716
The expression of ALDOA and/or SULT1A3 is significantly higher. PMID: 22949271
The results presented here point to ALDA as a factor involved in the regulation of cells proliferation. PMID: 23886627
The new prognostic biomarkers GRP78, Fructose-bisphosphate Aldolase A (ALDOA), Carbonic Anhydrase I (CA1) and Peptidyl-prolyl cis-trans isomerase A or Cyclophilin A (PPIA)) provided good survival prediction for TNM stage I-IV patients. PMID: 22996014
ALDOA is a target gene of the BACH1 transcription factor according to ChIP-seq analysis in HEK 293 cells. PMID: 21555518
Data that aldolase forms a complex with ARNO/Arf6 and the V-ATPase and that it may contribute to remodeling of the actin cytoskeleton. PMID: 21307348
Posttranslational nitration of aldolase A may be an important pathway that regulates mast cell phenotype and function. PMID: 20511553
ALDOA is involved in keratinocyte migration following the induction of lamellipodia formation, and ALDOA-related migration is enhanced by EGF PMID: 20362419
melanoma antigen expressed in G361, a representative melanoma cell line/ reacted with autoantibodies in patient sera PMID: 20181627
Gly346 is crucial for the correct conformation and function of aldolase A, because it governs the entry/release of the substrates into/from the enzyme cleft, and/or allows important C-terminal residues to approach the active site. PMID: 14766013
The existence of highly AMP-sensitive muscle-like FBPase, activity of which is regulated by metabolite-dependent interaction with aldolase enables the precise regulation of muscle energy expenditures. PMID: 18214967
Results identify VDAC2 and aldolase A as membrane proteins of K562 cells with increased expression under iron deprivation. PMID: 18278581
Aldolase A may play a role in the radio-response of human cells, probably in nuclei, in addition to its glycolytic role in the cytosol. PMID: 18328256
ZNF224 recruits the arginine methyltransferase PRMT5 on the transcriptional repressor complex of the aldolase A gene PMID: 19741270

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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