Recombinant Human GATC Protein

Name: Recombinant Human GATC Protein
Brand: Beta LifeScience
SKU: BL-1955PS
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	N/A
Host Species	Human
Synonym	Glutamyl-TRNA(Gln) Amidotransferase Subunit C, Glu-AdTSubunit C, 15E1.2, Glutamyl-TRNA(Gln) Amidotransferase Subunit C Homolog(Bacterial), Glutamyl-TRNA(Gln) Amidotransferase Subunit C Mitochondrial, Glutamyl-TRNA(Gln)Amidotransferase Subunit C Homolog, EC 6.3.5, Protein 15E1.2.
Background	Glutamyl-TRNAAmidotransferase, Subunit C also known as GATC allows the formation of properlycharged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) inthe mitochondria. The reaction occurs in the attendance of glutamine and ATP throughan activated gamma-phospho-Glu-tRNA(Gln). In addition, GATC is subunit of theheterotrimeric GatCAB amidotransferase (AdT) complex, composed of A (QRSL1), B (PET112)and C (GATC) subunits.
Description	GATC Human Recombinant expressed in E.Coli is a single, non-glycosylated polypeptide chain containing 159a.a. (1-136 a.a) and having a molecular weight of 17.5kDa (Molecular size on SDS-PAGE will appear higher).
Source	E.coli
AA Sequence	MGSSHHHHHH SSGLVPRGSH MGSMWSRLVW LGLRAPLGGR QGFTSKADPQ GSGRITAAVI EHLERLALVDFGSREAVARL EKAIAFADRL RAVDTDGVEP MESVLEDRCL YLRSDNVVEG NCADELLQNS HRVVEEYFVAPPGNISLPKL DEQEPFPHS
Purity	>85.0% as determined by SDS-PAGE.
Endotoxin	<1.0 EU per μg by the LAL method.
Formulation	GATC protein solution (0.25mg/ml) containing 20mMTris-HCl buffer (pH 8.0), 0.15M NaCl, 30% glycerol and 1mM DTT.
Stability	Recombinant protein is stable for 12 months at -70°C
Usage	For Research Use Only
Storage	Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.