Recombinant Human GLIPR1 Protein (C-6His)

Name: Recombinant Human GLIPR1 Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-0568NP
Availability: InStock

BL-0568NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Description	Recombinant Human Glioma Pathogenesis-Related Protein 1 is produced by our Mammalian expression system and the target gene encoding Asn22-Arg232 is expressed with a 6His tag at the C-terminus.
Accession	P48060
Synonym	Glioma Pathogenesis-Related Protein 1; GliPR 1; Protein RTVP-1; GLIPR1; GLIPR; RTVP1
Gene Background	Glioma Pathogenesis-Related Protein 1 (GLIPR1) is a member of the CRISP family. It is a single-pass membrane protein with 266 amino acids. GLIPR1 is highly expressed in the lung and kidney and lowly expressed in the heart and liver. It is also highly expressed in cell lines that are derived from nervous system tumors arising from glia; conversely, it is lowly expressed in non-glial-derived nervous system tumor cell lines. GLIPR1 is only expressed in brain tumor glioblastoma multiforme/astrocytoma and not expressed in other nervous system tumors nor normal adult or fetal tissues.
Molecular Mass	25.16 KDa
Apmol Mass	25-30 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Subcellular Location	Membrane; Single-pass membrane protein.
Protein Families	CRISP family
Database References	HGNC: 17001 OMIM: 602692 KEGG: hsa:11010 STRING: 9606.ENSP00000266659 UniGene: Hs.205558
Tissue Specificity	According to PubMed:8973356, it is ubiquitously expressed with high levels in lung and kidney and low levels in heart and liver. Highly expressed in cell lines derived from nervous system tumors arising from glia, low or absent in non-glial-derived nervou

Gene Functions References

Data found that GLIPR1 expression level was low in human bladder cancer tissues. Also, GLIPR1 was shown to inhibit the expression of TPX2. PMID: 28799673
High GLIPR1 expression is associated with cisplatin resistance in lung cancer. PMID: 28771580
The present study identified GLIPR1 and miR-16 as key components for regulating the proliferation, migration, invasion and cancer-initiating cells in osteosarcoma PMID: 27460987
This study reveals a novel pathway that PRMT5/WDR77 regulates GLIPR1 expression to control lung cancer cell growth. PMID: 26988096
Overexpression of RTVP-1 in human neural stem cells induced mesenchymal differentiation, whereas silencing of RTVP-1 in glioma stem cells (GSCs) decreased the mesenchymal transformation and stemness of these cells. PMID: 26267319
RTVP-1 regulates glioma cell spreading, migration and invasion and that these effects are mediated via interaction with N-WASP and by interfering with the inhibitory effect of hnRNPK on the function of this protein. PMID: 26305187
Data indicate that combining adenoviral vector-mediated GLIPR1 gene therap (AdGLIPR1) with radiotherapy may achieve additive or synergistic tumor control in selected prostate and bladder tumors. PMID: 23433894
Based on the observed overexpression in AML samples, GliPR1 should be further explored as a potential target for AML PMID: 24008279
miR-137 inhibits Glioblastom stem cell self-renewal and promotes their differentiation by targeting RTVP-1 which down-regulates CXCR4. PMID: 23714687
GLIPR1 interacts with Hsc70, and GLIPR1 overexpression or Hsc70 knockdown leads to transcriptional suppression of AURKA and TPX2. PMID: 23333597
GLIPR1 core structure has a conserved central cavity via which CAP proteins are likely to bind to shared ligands. PMID: 21931216
Data show that the expression of GLIPR1 and c-Myc were inversely correlated in prostate cancer. PMID: 22025562
RTVP-1 plays a role in the effect of serum response factor on glioma cell migration PMID: 21777672
demonstrate that GLIPR1 is a methylation-silenced gene in the acute myeloid leukemia patients PMID: 21922325
GLIPR1 expression is downregulated or even lost by promoter methylation in acute myeloid leukemia. PMID: 19483285
X-ray data have been collected to beyond 1.9 A resolution from a crystal of GLIPR1 that belonged to the orthorhombic space group P2(1)2(1)2 with average unit-cell parameters a = 85.1, b = 79.5, c = 38.9 A and either a monomer or dimer PMID: 21045302
Induction of GliPR expression by HIV-1 was confirmed in P4-CCR5 cells. PMID: 20356381
Expression of the human RTVP-1 gene is down-regulated in human prostate cancer specimens compared with normal human prostate tissue at the mRNA and protein levels. PMID: 14871827
In this study we report the identification and characterization of a novel p53 target gene cluster that includes human RTVP1 (hRTVP-1) together with two GLIPR1L genes (GLIPR1L1 and GLIPR1L2) on human chromosome 12q21 and mouse Rtvp1 PMID: 16714093
RTVP-1b is a novel splice variant of human RTVP-1, which was isolated from the U87 glioma cell line PMID: 17825796
a role for GLIPR1/RTVP-1 deregulation early in Wilms tumorigenesis. PMID: 18030365
GLIPR1 is a proapoptotic tumor suppressor acting through the ROS-JNK pathway. PMID: 18199537

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.