Recombinant Human GNRH2 Protein (C-6His)

Name: Recombinant Human GNRH2 Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-0638NP
Availability: InStock

BL-0638NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Description	Recombinant Human Progonadoliberin-2 is produced by our Mammalian expression system and the target gene encoding Gln24-Val120 is expressed with a 6His tag at the C-terminus.
Accession	O43555
Synonym	Progonadoliberin-2; Progonadoliberin II; Gonadoliberin-2; Gonadoliberin II; Gonadotropin-Releasing Hormone II; GnRH II; Luliberin II; Luteinizing Hormone-Releasing Hormone II; LH-RH II; GnRH-Associated Peptide 2; GnRH-Associated Peptide II; GNRH2
Gene Background	Progonadoliberin-2, also known as Progonadoliberin II and GNRH2, belongs to the gonadotropin-releasing hormone family. GNRH2 is specially expressed in midbrain, at significantly higher levels outside the brain (up to 30-fold). GNRH2 can be cleaved into two chains, gonadoliberin-2 and GnRH-associated peptide 2. gonadoliberin-2 regulates reproduction in females by stimulating the secretion of both luteinizing- and follicle-stimulating hormones. The proproteins produce three transcript variants, but produce the same peptide hormone.
Molecular Mass	11.6 KDa
Apmol Mass	14-25 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Stimulates the secretion of gonadotropins; it stimulates the secretion of both luteinizing and follicle-stimulating hormones.
Subcellular Location	Secreted.
Protein Families	GnRH family
Database References	HGNC: 4420 OMIM: 602352 KEGG: hsa:2797 STRING: 9606.ENSP00000245983 UniGene: Hs.129715
Tissue Specificity	Midbrain; expressed at significantly higher levels outside the brain (up to 30-fold), particularly in the kidney, bone marrow and prostate.

Gene Functions References

GnRHII expression correlated significantly with poor prognosis in breast cancer patients. PMID: 23287110
results suggest GnRH-II-induced laminin receptor precursor expression increases 67-kDa nonintegrin laminin receptor, which appears to interact with laminin in the extracellular matrix to promote MMP-2 expression and enhance ovarian cancer cell invasion PMID: 21193558
concluded that GnRHII stimulates PI3K/Akt pathway, and the phosphorylation of glycogen synthase kinase3beta, thereby enhancing the beta-catenin-dependent up-regulation of MT1-MMP production which contributes to ovarian cancer metastasis PMID: 21239435
These preliminary data suggest that local GnRH-II may participate in the regulation of ovarian tumor growth in post-menopausal women. PMID: 20018426
a novel mechanism in stimulating basal human GnRH-II gene transcription mediated by cooperative actions of multiple regulatory elements within the untranslated first exon of the gene. PMID: 12663744
GnRH-II has been identified, together with a 'putative' type II GnRH receptor, both in the central nervous system and in peripheral structures, such as tissues of the reproductive tract (both normal and tumoral). PMID: 14726258
GnRH-II did not affect proliferation of normal B-cells alone, and did not alter the proliferative response to IL-2. PMID: 15578334
Expression of GnRH2 protein and receptor (GnRHR2) is confirmed in both leiomyoma and patient-matched myometrium. PMID: 17296196
these data demonstrate the differential roles of NF-kappaB p65 and RARalpha/RXRalpha, interacting with the same sequence in the promoter of the human GnRH II gene to influence gene expression in a cell-specific manner. PMID: 17451432
GnRH II is widely expressed in prostate cancer and is an AR-regulated gene. PMID: 17914092
p-CREB, C/EBPbeta, and CBP are recruited to the CRE of the GnRH-II promoter in a temporarily defined manner to enhance its transcription in JEG-3 and OVCAR-3 cells in response to cAMP PMID: 18599546
These results provide evidence that EGF is an upstream regulator of the autocrine actions of GnRH-II on the invasive properties of ovarian cancer cells. PMID: 19608641

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.