Recombinant Human HLA-G Free Heavy Chain Protein, Active

Name: Recombinant Human HLA-G Free Heavy Chain Protein, Active
Brand: Beta LifeScience
SKU: BLK-02017P-100UG
Availability: InStock

Human HLA-G Free heavy chain on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Collections: Recombinant proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Description	Recombinant Human HLA-G Free Heavy Chain Protein is expressed from E.coli with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(C66S).
Purity	> 95% as determined by Tris-Bis PAGE
Accession	P17693-1
Target Symbol	HLA-G Free Heavy Chain
Synonyms	HLA G antigen; sHLA-G; b2 microglobulin; HLA G; HLAG; HLA-G; MHC Class I Antigen G; MHC class Ib antigen; MHC-G; sHLA-G
Species	Human
Expression System	E. coli
Tag	C-His-Avi
Expression Range	Gly25-Thr305(C66S)
Mol. Weight	The protein has a predicted MW of 35.5 kDa. The protein migrates to 36-40 kDa based on Tris-Bis PAGE result.
Form	Liquid
Formulation	Supplied as 0.22um filtered solution in 20mM Tris, 500mM NaCl, 20% Glycerol (pH 8.0).
Endotoxin	Less than 1EU per ug by the LAL method.
Activity	Immobilized Human HLA-G Free Heavy Chain, His Tag at 2ug/ml (100ul/Well) on the. Dose response curve for Anti-HLA-G Antibody, hFc Tag with the EC50 of 29.0ng/ml determined by ELISA (QC Test). Contact us for detailed testing images.
Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	Shipped with dry ice.
Gene Background	HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.