Recombinant Human Keratin, Type I Cytoskeletal 19 I (KRT19) Protein (His&Myc)

Name: Recombinant Human Keratin, Type I Cytoskeletal 19 I (KRT19) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-00072P
Price: 349.0 USD
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

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100ug

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Product Overview

Description	Recombinant Human Keratin, Type I Cytoskeletal 19 I (KRT19) Protein (His&Myc) is produced by our Baculovirus expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	P08727
Target Symbol	KRT19
Synonyms	40 kDa keratin intermediate filament; CK 19; CK-19; CK19; Cytokeratin 19; Cytokeratin-19; K19; K1C19_HUMAN; K1CS; Keratin 19; Keratin type I 40 kD; Keratin type I 40kD; Keratin type I cytoskeletal 19; Keratin, type I cytoskeletal 19; Keratin, type I, 40 kd; Keratin-19; KRT19; MGC15366
Species	Homo sapiens (Human)
Expression System	Baculovirus
Tag	N-10His&C-Myc
Target Protein Sequence	MTSYSYRQSSATSSFGGLGGGSVRFGPGVAFRAPSIHGGSGGRGVSVSSARFVSSSSSGAYGGGYGGVLTASDGLLAGNEKLTMQNLNDRLASYLDKVRALEAANGELEVKIRDWYQKQGPGPSRDYSHYYTTIQDLRDKILGATIENSRIVLQIDNARLAADDFRTKFETEQALRMSVEADINGLRRVLDELTLARTDLEMQIEGLKEELAYLKKNHEEEISTLRGQVGGQVSVEVDSAPGTDLAKILSDMRSQYEVMAEQNRKDAEAWFTSRTEELNREVAGHTEQLQMSRSEVTDLRRTLQGLEIELQSQLSMKAALEDTLAETEARFGAQLAHIQALISGIEAQLGDVRADSERQNQEYQRLMDIKSRLEQEIATYRSLLEGQEDHYNNLSASKVL
Expression Range	1-400aa
Protein Length	Full length
Mol. Weight	48
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Protein Families	Intermediate filament family
Database References	HGNC: 6436 OMIM: 148020 KEGG: hsa:3880 STRING: 9606.ENSP00000355124 UniGene: Hs.654568
Tissue Specificity	Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagu

Gene Functions References

elevated level of preoperative serum Cyfra 21-1 indicates an advanced stage of tumor PMID: 30037371
CYFRA 21-1 assessed at the end of RT or ChT seems to be a prognostic marker for tumor response. A high concentration of CYFRA 21-1 after treatment increases the risk of death. CYFRA 21-1 might be suggested in the monitoring of carcinomas of head and neck cancer (HNC). PMID: 28604997
the modulation of KRT19 expression could be involved in cancer stem cell reprogramming and drug sensitivity. PMID: 29747452
High expression of CK-19 is associated with intrahepatic cholangiocarcinoma. PMID: 29513894
KRT19 intracellularly binds to HER2, playing a critical role in HER2 activation PMID: 28008968
The combined use of CK19 and CD56 is helpful in discriminating papillary thyroid carcinoma and its variants from other mimicking thyroid lesions. PMID: 28214214
CYFRA 21-1 can be useful as a tumor marker for evaluating tumor progression and treatment efficacy in patients with extramammary Paget's disease. PMID: 28150330
YAP1 activation was more commonly found in CCAs than in pure HCCs. However, a differing pattern of YAP1 expression between cHC-CCAs and CK19(+) HCCs and the poor prognosis of YAP1 positive hepatic carcinomas suggests that YAP1 may have a preferential role in aggressive tumor behavior, rather than in the determination of cellular lineage in hepatic carcinomas PMID: 28645247
Circulating tumor cells expressing cytokeratin and tumor-initiating cell markers, including ALDH, CD133, and CD44, were identified in patients with pancreatic adenocarcinoma. These TIC-like CTCs were associated with poor prognosis after surgical resection and with an increased incidence of tumor recurrence PMID: 27789528
Aberrant decreased miR-26a could promote cell proliferation by regulating KRT19 which plays important role in the pathogenesis of cholangiocarcinoma. PMID: 27833076
The detection of TK1 combined with that of cytokeratin-19 fragment (CYFRA21-1), CEA or NSE increased the diagnostic value of TK1 for lung squamous cell carcinoma, adenocarcinoma and small cell lung cancer, respectively. PMID: 29247745
Our results are of special importance against the background that CK19 positive carcinomas revealed a significantly poorer prognosis and therefore emphasize its prognostic and possible diagnostic role in tongue cancer. PMID: 27764819
(18)F-FDG-PET can be used to predict K19 expression in hepatocellular carcinoma and should thereby aid in the development of novel therapeutic strategies targeting K19(+) hepatocellular carcinoma cancer stem cells PMID: 27663597
Immunohistochemical assessment of both CK19 and SCCAg status in histologically negative sentinel lymph nodes may be a valuable approach for predicting recurrence and survival after curative surgery for cervical squamous cell carcinoma. PMID: 27654256
Positive CK-19 expression can be used as a predictor of poor prognosis of PNET PMID: 29136022
Data suggest that high cytokeratin 19 (CK19) levels in peripheral blood may independently predict non-sentinel lymph node (nSLN) metastasis in breast cancer patients. PMID: 27105542
Findings provide evidence that K19 expression is inducible in human hepatocellular carcinoma as a sign of tumor progression. PMID: 28783171
Urine levels of COL4A1, COL13A1, the combined values of COL4A1 and COL13A1 (COL4A1 + COL13A1), and CYFRA21-1 were significantly elevated in urine from patients with BCa compared to the controls. A high urinary COL4A1 + COL13A1 was found to be an independent risk factor for intravesical recurrence. PMID: 28837258
CK19 and carcinoembryonic antigen may be involved in lymph node metastasis and poor prognosis in patients with gastriccardia cancer PMID: 28293089
The study has confirmed the preservation of CK19 protein expression in breast cancer cells after neoadjuvant therapy. On the basis of these results, quantification-based methods such as the one-step nucleic acid amplification (OSNA) CK19 assay, could be an accurate tool with which to analyse the sentinel nodes, regardless of whether they had been obtained before or after treatment. PMID: 26708058
Serum CYFRA 21-1 level may be a predictive factor for patients with non-small cell lung cancers treated with EGFR-TKIs, regardless of EGFR mutation status. PMID: 28982900
Data suggest that keratin (K) 19 (K19) may be a drug target for the treatment of K19-positive hepatocellular carcinoma (HCC). PMID: 27863477
Combination of the two tumor markers, CEA and CYFRA21-1, is a predictive and prognostic marker of docetaxel monotherapy for previously treated NSCLC patients PMID: 28870944
Results suggest that positive expression of CK19 mRNA and TK1 protein is closely correlated with poor prognosis in advanced gastrointestinal cancer. PMID: 27625087
GPC3 and KRT19 overexpression are associated with carcinogenesis, progression, and poor prognosis in patients with PDAC and a valuable biomarker for diagnosis of PDAC. PMID: 27689616
Data show that sixty one patients with a high cytokeratin 19 fragment (CYFRA 21-1) level had statistically shorter progression-free and overall survival than 39 patients with a normal CYFRA 21-1 level . PMID: 28179355
Data indicate that serum tumor markers showed significant shorter 3-year progression-free survival (PFS) in higher levels compared to lower levels for S-CYFRA 21-1 (cytokeratin 19 fragment), S-SCCA and S-CEA. PMID: 26432331
Gene promoter hypermethylation is found in sentinel lymph nodes of breast cancer patients identified as positive by one-step nucleic acid amplification of cytokeratin 19 mRNA PMID: 27097811
Peritumoral ductular reaction was more abundant and proliferative in CK19+ hepatocellular carcinoma patients, with higher level of nuclear translocation of beta-catenin. PMID: 27049492
BALF and serum NSE, CEA, and CYFRA21.1 elevated in lung cancer, which had prompt value for pathology, especially significant for BALF. PMID: 27072263
Significant levels of CEA, CYFRA 21-1, NSE, and TSGF were detected in the serum. The amounts found were useful for diagnosing non-small cell lung cancer (NSCLC) patients who depended on the currently limited biomarker development. PMID: 27072222
The cytologic tumor marker c-CYFRA was positively associated with EGFR mutations in non-small cell lung cancer (NSCLC). EGFR mutation-positive NSCLCs have relatively lower glycolysis compared with NSCLCs without EGFR mutation PMID: 26979333
Results show that serum miR21 could serve as an important marker for auxiliary diagnosis of early non-small cell lung cancer (NSCLC), while joint detection of serums miR21 and CYFRA21-1 levels could improve diagnostic efficiency. PMID: 26880855
Positive staining for CK19 suggests a possible LPC origin of the EpCAM positive hepatocellular carcinoma . PMID: 26609030
Increased Expression of K19 mRNA is associated with Recurrence After Curative Resection of Hepatocellular Carcinoma. PMID: 25791790
Patients with CK19 positivity showed a higher prevalence of microvessel invasion than those without CK19. PMID: 26212571
We report results of the studies relating to fabrication of a non-invasive, label-free and an efficient biosensing platform for detection of the oral cancer biomarker (CYFRA-21-1). PMID: 26657594
Serum CYFRA21-1 and CEA can be used as prognostic factors of NSCLC patients. Combinative detection of the two indices will be more reliable. PMID: 26333429
Data show that serum neuron-specific enolase, cytokeratin 19 fragment 21-1, pro-gastrin-releasing peptide, squamous cell carcinoma antigen, tissue inhibitor of metalloproteinase-1, and human epididymis protein 4 are not associated with brain metastases. PMID: 26730601
CK19 expression was measured in hepatocellular carcinoma at different stages and correlated with prognosis. CK19+/GPC3+ HCC has the highest risk of intrahepatic metastasis, microvascular invasion, regional lymph node involvement, and distant metastasis. PMID: 26977595
CK19 staining was found in hepatic hydatidosis patients with and without biliary fistula. PMID: 27141570
When confirmed by a low alpha-fetoprotein concentration (<400 mug/L), CK-19 expression in is an additional valuable indicator of early recurrence, distant metastasis, and poor prognosis in HBV-positive patients PMID: 26588210
Study confirms the prognostic significance of K19 expression in Caucasian patients with hepatocellular carcinoma. PMID: 26011233
High serum levels of CYFRA 21-1 are associated with oral squamous cell carcinoma. PMID: 26292957
K19 is a new cancer stem cell marker associated with epithelial mesechymal transformation and TGFb/Smad signaling. PMID: 25820415
Elevation of serum CYFRA21-1 is associated with Non-small Cell Lung Cancer. PMID: 26163610
Postoperative CK19-2G2 concentration may be an indicator of prognosis. An increase after the initial drop in CK19-2G2 concentration after surgery may indicate a poor prognosis. PMID: 26002573
CK19 and HBME-1 were significantly expressed more in papillary carcinoma as compared to follicular carcinoma PMID: 26503236
CK19 mRNA expression in peripheral blood was evaluated by qRT-PCR as an index of circulating tumor cells in 15 cases of HER-2-positive breast cancer and 18 cases of HER2-negative breast cancer PMID: 25867356
CK19 proved to be a sensitive marker to identify yolk sac tumours of the testis and to distinguish them from other germ cell tumours. PMID: 25889715

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