Recombinant Human LBP Protein (C-6His)

Name: Recombinant Human LBP Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-0431NP
Availability: InStock

BL-0431NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Description	Recombinant Human Lipopolysaccharide-Binding Protein is produced by our Mammalian expression system and the target gene encoding Ala26-Val481 is expressed with a 6His tag at the C-terminus.
Accession	P18428
Synonym	Lipopolysaccharide-Binding Protein; LBP
Gene Background	Lipopolysaccharide binding protein (LBP) is a plasma protein, belongs to a member of structurally and functionally related proteins which includes bactericidal permeability-increasing protein (BPI), plasma cholesteryl ester transfer protein (CETP) and phospholipid transfer protein (PLTP). It is involved in the acute-phase immunologic response to gram-negative bacterial infections. In cooperation with BPI. LBP binds LPS and interacts with the CD14 receptor, most likely playing a role in regulating LPS-dependent monocyte responses. Studies suggest that LBP is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS from circulation. Finally, t The LBP gene is found on chromosome 20, directly downstream of the BPI gene.
Molecular Mass	51.95 KDa
Apmol Mass	64 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, 1mM EDTA, pH 8.0.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide.
Subcellular Location	Secreted. Cytoplasmic granule membrane.
Protein Families	BPI/LBP/Plunc superfamily, BPI/LBP family
Database References	HGNC: 6517 OMIM: 151990 KEGG: hsa:3929 STRING: 9606.ENSP00000217407 UniGene: Hs.154078
Tissue Specificity	Detected in blood serum (at protein level).

Gene Functions References

This study shows a remarkable reversal of amyloid fibrin formation by LBP addition to the plasma of Parkinson's Disease patients . PMID: 29494603
Serum LBP levels are associated with arterial stiffness, independent of obesity and traditional cardiovascular risk factors, especially in men with type 2 diabetes. PMID: 28486964
novel observation that sCD14 compared with lipopolysaccharide binding protein, offers a preferred target to ameliorate TLR especially TLR4-induced inflammation and insulin resistance in human obesity and metabolic syndrome PMID: 26880233
LBP, an endotoxemia associated protein might be used as an inflammatory biomarker of both infectious and non-infectious origins in HCV-infected subjects PMID: 28107471
Data show that after matching for gender, age, and body mass index (BMI), serum lipopolysaccharide-binding protein (LBP) does not improve prediction of the development of type 2 diabetes mellitus (T2DM) independently. PMID: 25753130
The main findings of this study are that, in acute stroke patients, levels of LBP, IL-10, IL-6 and CRP show a different time course in patients with and without post-stroke infection. PMID: 25613713
Serum LBP level is significantly elevated in polycystic ovary syndrome women and is associated with insulin resistance. PMID: 26799617
LBP serves not only as an extracellular LPS shuttle but in addition facilitates intracellular transport of LPS. PMID: 26804480
LBP level was not significantly different in neutropenic systemic inflammatory response syndrome patients and sepsis patients. PMID: 26046926
Report increased secretion of Fetuin A, LBP and HMGB-1 from subcutaneous adipose tissue in metabolic syndrome. PMID: 25978344
Low levels of microbial translocation marker LBP are associated with sustained viral response after anti-HCV treatment in HIV-1/HCV co-infected patients PMID: 25785448
serum level elevated in late-onset neonatal sepsis in very low birth weight infants PMID: 25014514
Adjusting for body mass index and waist circumference, LBP levels remained significantly increased in metabolic syndrome and increased with increasing numbers of MetS risk factors. PMID: 25063948
study suggested that elevated plasma LBP was associated with an increased risk of developing MetS among middle-aged and older Chinese, especially in normal-weight individuals. PMID: 24906952
In preterm neonates born to asymptomatic women with PPROM, LBP in cord blood at delivery is an excellent diagnostic biomarker of Fetal inflammatory response syndrome/funisitis with prognostic potential. PMID: 24335151
report pre-ART IL-6 and LBP levels as well as IL-6, LBP and I-FABP levels during IRIS-event as potential biomarkers in TB-IRIS PMID: 24312369
In childnre, LBP was independently associated with BMI and with measures of obstructive sleep apnea severity as well as with metabolic dysfunction, particularly insulin resistance as indicated by the homeostasis model assessment of insulin resistance. PMID: 24276451
Suggest that the serum LBP concentration constitutes a reliable biologic marker for the diagnosis of a febrile urinary tract infection in children. PMID: 23463341
Mutations in the lipopolysaccharide binding protein impair innate immunity. PMID: 24120359
Serum LBP concentrations increased with age. Overweight, obesity, and having metabolic syndrome (particularly, low HDL cholesterol levels) were associated with higher LBP concentrations PMID: 23349936
expression in oral keratinocytes is stimulated by Porphyromonas gingivalis LPS PMID: 22736337
Human lipopolysaccharide-binding protein (LBP) and CD14 independently deliver triacylated lipoproteins to Toll-like receptor 1 (TLR1) and TLR2 and enhance formation of the ternary signaling complex PMID: 23430250
LBP is a surrogate marker of microbial translocation in association with physical functioning of older adults. PMID: 22960476
Association of lipopolysaccharide-binding protein gene polymorphisms with cerebral infarction in a Chinese population PMID: 22476641
our study demonstrated that an elevated LBP level of >15000 ng/ml may serve as a biomarker for the prediction and monitoring of aGVHD. PMID: 22552880
During the first 14 days of postoperative sepsis, LBP plasma concentrations showed a time course that was very similar to CRP with a high concordance in the pattern of day-to-day changes PMID: 21901123
The availability of commercial methods for the automated measurement of the soluble CD14 subtype presepsin and lipopolysaccharide binding protein represent a challenge for the evaluation in clinical practice of reliable markers of neonatal sepsis. PMID: 21740312
Aseptic trauma primes the innate immune system for the posttraumatic release of lipopolysaccharide binding protein and sCD14 PMID: 21722015
Cytokine concentrations in amniotic fluid during the mid-trimester did not differ with parity or fetal gender. IL-6, IL-8, and LBP levels appeared stable with gestational week (GW), whereas GW significantly influenced TNF-alpha concentrations. PMID: 21702700
LBP measurements performed shortly after preterm premature rupture of membranes, are not of value either in the prediction of newborn's infection, or in the prognosis of latency period duration. PMID: 21353369
This study highlights the LBP rs2232596 and CD14 rs4914 polymorphisms as biomarkers for elevated colorectal carcinoma susceptibility in the Chinese Han population PMID: 21633598
High LBP is associated with Crohn's disease. PMID: 20865702
Elevated circulating LBP was associated with obesity, metabolic syndrome, and type 2 diabetes in apparently healthy Chinese. PMID: 20530747
The association of the polymorphisms c.291C>T and c.613A>G suggest a role of LBP in the disease manifestation of infective endocarditis. PMID: 19560454
Serial LBP serum measurements may offer a clinically useful biomarker for identification of patients with severe sepsis having the worst outcomes and the highest probability of developing sepsis-induced ARDS PMID: 19718443
carboxyl-terminal domain of these closely related endotoxin-binding proteins dictates the route and host responses to complexes they form with endotoxin. PMID: 11773072
a new and reliable infection marker after kidney transplantation PMID: 11976738
Identification of single amino acid residues essential for the binding of lipopolysaccharide (LPS) to LPS binding protein (LBP) residues 86-99 by using an Ala-scanning library PMID: 11991204
plasma factor LBP and cell surface receptor CD14 were necessary for LPS activation of p38, which was tightly associated with LPS priming of the PMN respiratory burst PMID: 12117913
Besides a role in the detoxification of bacterial toxin present in the circulation, LBP-chylomicron complexes may be part of a local defense mechanism of the intestine against translocated bacterial toxin. PMID: 12538700
innate immune recognition of LTA via LBP, CD14, and TLR-2 represents an important mechanism in the pathogenesis of systemic complications in the course of infectious diseases brought about by Gram-positive pathogens. while TLR-4 and MD-2 are not involved. PMID: 12594207
Single nucleotide polymorphism of the LBP gene is not assciated with complicated sepsis after trauma. PMID: 12615620
data support the hypothesis that lipopolysaccharide binding protein can inhibit cell responses to lipopolysaccharide(LPS) by inhibiting LPS transfer from membrane CD14 to the Toll-like receptor 4-MD-2 signaling receptor PMID: 12754215
plays an essential role in the innate immune response to Gram-positive pneumococci PMID: 12932360
In critically ill neonates aged over 48 h and children lipopolysaccharide binding protein is a better marker than procalcitonin, interleukin-6 and C-reactive protein. PMID: 15127192
Dual role of LBP and CD14 in initiation of proinflammatory signaling and clearance or neutralization of LPS. PMID: 15618154
studies suggest that SP-A could contribute to modulate Re-LPS responses by altering the competence of the LBP-CD14 receptor complex PMID: 15932345
LBP mediates the fusion of lipid membranes and LPS aggregates. PMID: 16303759
Human pulmonary LBP acts as an important modulator of the LPS response in the respiratory tract in vivo. PMID: 16493079
Significantly elevated serum concentrations of LBP and sCD14 are found in severe sepsis patients. PMID: 16512634

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

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