Recombinant Human LEC Protein (His Tag)

Name: Recombinant Human LEC Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-3162
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Tag	His
Host Species	Human
Accession	NP_004581.1
Synonym	CCL16, CKb12, HCC-4, ILINCK, LCC-1, LEC, LMC, Mtn-1, NCC-4, NCC4, SCYA16, SCYL4
Background	CCL16, a chemokine poorly characterized at thefunctional level. Human CCL16 is a member of the CCfamily, and its gene maps to human chromosome 17q. In themouse, only a pseudogene has been identified to date.CCL16 is a functional ligand for CCR1, CCR2, CCR5, andCCR8. Recombinant CCL16 demonstrated chemotactic activityon human monocytes and lymphocytes. Based on the abilityof human chemokines to exert activity on and bind to murinereceptors, the TSA mouse adenocarcinoma cell line was transfectedwith human CCL16 cDNA and, in comparison with othercytokines, was shown to be the faster inducer of systemicimmune response due to massive, prompt infiltration of leukocytes.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy
Description	A DNA sequence encoding human CCL16 (NP_004581.1) (Gln24-Gln120) was expressed with a His tag at the C-terminus.
Source	Yeast
Predicted N Terminal	Gln 24
AA Sequence	Gln24-Gln120
Molecular Weight	The recombinant human CCL16 consists of 107 a.a. and predicts a molecular mass of 12.6 KDa. It migrates as an approximately 15 KDa band in SDS-PAGE under reducing conditions.
Purity	>95% as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile PBS, pH 7.4..
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.