Recombinant Human Metalloreductase Steap2 (STEAP2) Protein (His)

Name: Recombinant Human Metalloreductase Steap2 (STEAP2) Protein (His)
Brand: Beta LifeScience
SKU: BLC-06180P
Price: 299.0 USD
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: Beta lifescience's spring promotion, In-stock recombinant proteins, Other recombinant proteins, Recombinant proteins, Recombinant proteins fall special offers - full-length proteins, Recombinant proteins fall special offers - transmembrane proteins

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Size

100ug

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Product Overview

Description	Recombinant Human Metalloreductase Steap2 (STEAP2) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Activity	Not tested.
Uniprotkb	Q8NFT2
Target Symbol	STEAP2
Synonyms	Prostate cancer-associated protein 1;Protein up-regulated in metastatic prostate cancer;PUMPCn;Six-transmembrane epithelial antigen of prostate 2;SixTransMembrane protein of prostate 1
Species	Homo sapiens (Human)
Expression System	in vitro E.coli expression system
Tag	N-10His
Target Protein Sequence	MESISMMGSPKSLSETFLPNGINGIKDARKVTVGVIGSGDFAKSLTIRLIRCGYHVVIGSRNPKFASEFFPHVVDVTHHEDALTKTNIIFVAIHREHYTSLWDLRHLLVGKILIDVSNNMRINQYPESNAEYLASLFPDSLIVKGFNVVSAWALQLGPKDASRQVYICSNNIQARQQVIELARQLNFIPIDLGSLSSAREIENLPLRLFTLWRGPVVVAISLATFFFLYSFVRDVIHPYARNQQSDFYKIPIEIVNKTLPIVAITLLSLVYLAGLLAAAYQLYYGTKYRRFPPWLETWLQCRKQLGLLSFFFAMVHVAYSLCLPMRRSERYLFLNMAYQQVHANIENSWNEEEVWRIEMYISFGIMSLGLLSLLAVTSIPSVSNALNWREFSFIQSTLGYVALLISTFHVLIYGWKRAFEEEYYRFYTPPNFVLALVLPSIVILGKIILFLPCISRKLKRIKKGWEKSQFLEEGMGGTIPHVSPERVTVM
Expression Range	1-490aa
Protein Length	Full Length
Mol. Weight	57.6 kDa
Research Area	Cancer
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor.
Subcellular Location	Endosome membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Protein Families	STEAP family
Database References	HGNC: 17885 OMIM: 605094 KEGG: hsa:261729 STRING: 9606.ENSP00000287908 UniGene: Hs.489051
Tissue Specificity	Expressed at high levels in prostate and at significantly lower levels in heart, brain, kidney, pancreas, and ovary.

Gene Functions References

Over-expression of STEAP2 is associated with prostate cancer progression. PMID: 25248617
Data suggest that STAMP1 is required for prostate cancer growth. PMID: 20587517
Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
cloning and characterization of STAMP1, which is expressed in both normal prostate and prostate neoplasms PMID: 12095985
STEAP2 is involved in the development of prostate cancer. As a cell-surface antigen, it is a potential diagnostic or therapeutic target in prostate cancer. PMID: 12429817
Expression of STAMP2, a gene highly similar to STAMP1 (STEAP2), in prostate cancer cells significantly increases cell growth and colony formation suggesting that STAMP2 may have a role in cell proliferation. PMID: 15897894
STEAPs may represent novel markers of mesenchymal stem cells in man as well as mice. PMID: 19196137
The murine ortholog, Steap2, is a ferrireductase, cupric reductase, and stimulates cellular uptake of both iron and copper in vitro. PMID: 16609065

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.