Recombinant Human MFGE8 Protein (His & AVI Tag), Biotinylated

Name: Recombinant Human MFGE8 Protein (His & AVI Tag), Biotinylated
Brand: Beta LifeScience
SKU: BLPSN-3357
Availability: InStock

Collections: Avi-tagged proteins, Biotinylated proteins, Featured biotinylated protein molecules, Recombinant proteins

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Product Overview

Tag	His&AVI
Host Species	Human
Accession	Q08431
Synonym	BA46, EDIL1, HMFG, hP47, HsT19888, MFG-E8, MFGM, OAcGD3S, SED1, SPAG10
Background	MFG-E8, also known as lactadherin and MFGE8, contains 1 EGF-like domain and 2 F5/8 type C domains. It also contains a phosphatidylserine (PS) binding domain, as well as an Arginine-Glycine-Aspartic acid motif, which enables the binding to integrins. It binds PS, which is exposed on the surface of apoptotic cells. MFG-E8 is expressed in mammary epithelial cell surfaces and aortic media. Overexpression of MFG-E8 can be found in several carcinomas. MFG-E8 has an opsonization of the apoptotic cells and binding to integrins on the surface of phagocytic cells. It also mediates the engulfment of the dead cell. MFG-E8 plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. It promotes VEGF-dependent neovascularization and contributes to phagocytic removal of apoptotic cells in many tissues. It also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner.
Description	A DNA sequence encoding the human MFGE8 (Q08431.2) (Met1-Cys387) was expressed with a c-terminal His tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Source	Baculovirus-Insect Cells
Predicted N Terminal	Leu 24
AA Sequence	Met1-Cys387
Molecular Weight	The recombinant human MFGE8 consists of 390 a.a. and predicts a molecular mass of 44.1 kDa.
Purity	>95% as determined by SDS-PAGE.
Endotoxin	< 1.0 EU per μg protein as determined by the LAL method.
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile 20 mM Tris, 150 mM NaCl, pH 8.5, 10 % glycerol, 0.2 M Arginine, 0.01 % Tween-20..
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization. Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction.; Main constituent of aortic medial amyloid.
Subcellular Location	Membrane; Peripheral membrane protein. Secreted. Cytoplasmic vesicle, secretory vesicle, acrosome membrane; Peripheral membrane protein.
Database References	HGNC: 7036 OMIM: 602281 KEGG: hsa:4240 STRING: 9606.ENSP00000268150 UniGene: Hs.3745
Tissue Specificity	Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.

Gene Functions References

Esophageal cancer patients with high MFG-E8 expression had worse overall survival and relapse-free survival in the subgroup with preoperative chemotherapy. The abundant MFG-E8 expression in esophageal squamous cell carcinoma might have a negative influence on the long-term survival of patients after chemotherapy by affecting T-cell regulation in the tumor microenvironment. PMID: 30156356
MFGE8 polymorphisms were not associated with MetS but were related to DBP, HDL-C level, and WC in Chinese adults. PMID: 30053459
This study showed for the first time that MFG-E8 expression is impaired in the endometrium of patients with endometriosis and infertility during the window of implantation. PMID: 28967712
Our findings suggest that both MFGE8 and TGFbeta1 are agerelated inflammatory factors and are related to the degree of Atherosclerosis (AS). In conclusion, both MFGE8 and TGFbeta1 may serve as potential markers of the severity of AS PMID: 29152651
Elevated serum MFG-E8 levels may be associated with cerebrovascular diseases or neuropsychiatric systemic lupus erythematosus. PMID: 28266034
Circulating MFG-E8 levels are dramatically elevated in pregnancy, and are significantly higher in gestational diabetes mellitus than in normal pregnancy. PMID: 28035763
MFG-E8 could be a potential novel prognostic marker for colorectal cancer (CRC) and overexpression of MFG-E8 might be involved in lymph node metastasis and angiogenesis of CRC. PMID: 28923329
MFG-E8 levels are elevated in T2D but suppressed by increased adipose tissues, thereby allowing inflammatory factors to rise to high levels. MFG-E8 may serve as a potential biomarker for obesity and T2D in the clinical setting PMID: 28067023
These findings provide a better understanding of the molecular mechanism underlying colorectal cancer progression and suggest a predictive role for MFG-E8 in colorectal cancer metastasis and prognosis PMID: 28653875
Levels of MFGE8 were reduced in cirrhotic liver tissue from patients compared with controls. PMID: 27956229
an efficient purification method for production of non-aggregated, full-length MFG-E8. PMID: 27102803
Reduced serum milk fat globule-epidermal growth factor 8 (MFG-E8) concentrations are associated with an increased risk of microvascular complications in patients with type 2 diabetes. PMID: 28089751
MFG-E8 is a protective factor in the pathogenesis of rheumatoid arthritis and subsequent bone loss. PMID: 26391522
After myocardial infarction, Mfge8 (and Mertk)-expressing macrophages synergistically engage the clearance of injured cardiomyocytes. PMID: 26819373
Studied the therapeutic effect of rhMFG-E8 in mouse models of IBD. Treatment with rhMFG-E8 significantly attenuated colitis in both models in a dose-dependent way. PMID: 25751740
MFG-E8 promotes cutaneous wound healing by enhancing angiogenesis. PMID: 24838098
Lactadherin may decrease inflammation by inhibiting secretory phospholipase A2 activity on pre-apoptotic leukemia cells. PMID: 24194865
promotes tumor progression in oral squamous cell carcinoma PMID: 25264705
MFG-E8 had a negative association with hs-CRP and a positive association with LDL-c. The serum level of MFG-E8 was negatively associated with the severity of coronary artery stenosis and the risk of clinical events. PMID: 24561551
medin adopts a predominantly beta-sheet conformation with some unstructured elements. PMID: 24602872
MFG-E8 could be used as a biomarker for diagnosis and monitoring of disease activity in certain systemic lupus erythematosus patients PMID: 24554711
Exogenously added MFG-E8 inhibits receptor activator NF-kappaB ligand-induced osteoclastogenesis of human osteoclast precursors. PMID: 24958900
Our results strongly suggested that MFG-E8 is a promising biomarker for the diagnosis, prognosis, and therapy target of opisthorchiasis-associated cholangiocarcinoma PMID: 24122204
Blockage of MFG-E8 in endometrial tumor cells diminishes trophoblaast cell attachment. PMID: 24424369
TNF-alpha up-regulates endometrial epithelial cell migration and MFG-E8 production. PMID: 24262600
MFG-E8-dependent promotion of apoptotic cell clearance is a novel anti-inflammatory facet of glucocorticoid treatment PMID: 23832117
our data argue that MFGE8 is not likely involved in the phagocytic clearance of neuronal debris associated with nigrostriatal pathway injury. PMID: 23194669
NMR solution structure of C2 domain of MFG-E8 and insights into its molecular recognition with phosphatidylserine PMID: 23262193
Milk fat globule-epidermal growth factor 8 has proapoptotic activity, suggesting participation in endometrial remodeling via an epithelial-stromal cell paracrine effect. PMID: 22921913
MFG-E8 expression in the endometrial epithelium as well as in chorionic villi suggests its possible role in endometrial reorganization during the receptive phase and in events related to normal pregnancy in mammals PMID: 22770563
Decreased colonic MFGE8 expression in patients with ulcerative colitis may be associated with mucosal inflammatory activity and clinical disease activity through basal cell apoptosis and preventing tissue healing in the pathogenesis of ulcerative colitis PMID: 22204000
a key role of MFG-E8 release from apoptotic endothelial cells in macrophage reprogramming PMID: 22558449
The distribution of the SNP (rs4945 and rs1878326) of MFGE8 was analyzed in two groups of patients with "wet" age-related macular Degeneration and their age-matched controls. PMID: 22438901
serum lactadherin is correlated with poor blood glucose control and diabetic vascular complications. PMID: 22018779
We conclude that MFG-E8-dependent signaling stimulates cell proliferation and the acquisition of mesenchymal properties and contributes to mammary carcinoma development. PMID: 21841820
in vitro studies showed that medin amyloid-like fibrils promote the aggregation of protein amyloid A into fibrils PMID: 22070546
Prolactin has a modulatory role for as a stromal/epithelial paracrine factor controlling MFG-E8. This is the first report on MFG-E8 protein localization to the human endometrial epithelium and its up-regulation during the window of implantation. PMID: 21177637
MFG-E8 is expressed in triple-negative breast cancers as a target gene of the p63 pathway, but may serve a suppressive function in ER(+) and erbB2(+) breast cancers. PMID: 21127199
overexpression of MFGE8 during bladder tumor development, correlated with expression of genes involved in cell adhesion or migration and in immune responses. PMID: 20956946
intronic mutation in the human MFG-E8 gene can lead to the development of SLE. PMID: 20213738
SED1 is expressed on the surface of acrosome-intact human sperm and in the anterior caput of the human epididymis, similar to that seen in mouse PMID: 18990388
Truncated fragment of medin, the hexapeptide, NFGSVQ can form typical amyloid fibrils. PMID: 15478463
Might prove useful in the treatment of prolonged ischemia. PMID: 16115445
Lactadherin) is expressed in normal and atherosclerotic human arteries. PMID: 17420351
The trans-activator (TA) isoforms of p63 activate MFGE8 transcription though a p53/p63 motif at -370, which was confirmed by a chromatin immunoprecipitation experiment. PMID: 17637751
Identification of the last 18-19 amino acid residues as constituting the amyloid-promoting region of medin. PMID: 17679143
aggregated medin induced death of aortic smooth muscle cells in vitro. Cells incubated together with medin increased the production of matrix metalloproteinase-2, a protease that degrades elastin and collagen and subsequently weakens the vessel wall. PMID: 17906662
analysis of membrane-interactive loops of Lact-C2 PMID: 18160406
Some childhood-onset and adult SLE patients carried a significant level of MFG-E8 in their blood samples. PMID: 18303131
Lactadherin promoted phagocytosis of phosphatidylserine-expressing RBCs by macrophages in a concentration-dependent manner. PMID: 18647368

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