Recombinant Human Mothers Against Decapentaplegic Homolog 6 (SMAD6) Protein (His&Myc)

Name: Recombinant Human Mothers Against Decapentaplegic Homolog 6 (SMAD6) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-04981P
Price: 349.0 USD
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

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Size

100ug

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Product Overview

Description	Recombinant Human Mothers Against Decapentaplegic Homolog 6 (SMAD6) Protein (His&Myc) is produced by our Baculovirus expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	O43541
Target Symbol	SMAD6
Synonyms	hSMAD6; MAD homolog 6; MADH6; MADH7; Mothers against decapentaplegic homolog 6; Mothers against DPP homolog 6; SMAD 6; SMAD family member 6; SMAD mothers against DPP homolog 6; Smad6; SMAD6_HUMAN
Species	Homo sapiens (Human)
Expression System	Baculovirus
Tag	N-10His&C-Myc
Target Protein Sequence	MFRSKRSGLVRRLWRSRVVPDREEGGSGGGGGGDEDGSLGSRAEPAPRAREGGGCGRSEVRPVAPRRPRDAVGQRGAQGAGRRRRAGGPPRPMSEPGAGAGSSLLDVAEPGGPGWLPESDCETVTCCLFSERDAAGAPRDASDPLAGAALEPAGGGRSREARSRLLLLEQELKTVTYSLLKRLKERSLDTLLEAVESRGGVPGGCVLVPRADLRLGGQPAPPQLLLGRLFRWPDLQHAVELKPLCGCHSFAAAADGPTVCCNPYHFSRLCGPESPPPPYSRLSPRDEYKPLDLSDSTLSYTETEATNSLITAPGEFSDASMSPDATKPSHWCSVAYWEHRTRVGRLYAVYDQAVSIFYDLPQGSGFCLGQLNLEQRSESVRRTRSKIGFGILLSKEPDGVWAYNRGEHPIFVNSPTLDAPGGRALVVRKVPPGYSIKVFDFERSGLQHAPEPDAADGPYDPNSVRISFAKGWGPCYSRQFITSCPCWLEILLNNPR
Expression Range	1-496aa
Protein Length	Full Length
Mol. Weight	57.4
Research Area	Epigenetics And Nuclear Signaling
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Transforming growth factor-beta superfamily receptors signaling occurs through the Smad family of intracellular mediators. SMAD6 is an inhibitory Smad (i-Smad) that negatively regulates signaling downstream of type I transforming growth factor-beta. Acts as a mediator of TGF-beta and BMP anti-inflammatory activities. Suppresses IL1R-TLR signaling through its direct interaction with PEL1, preventing NF-kappa-B activation, nuclear transport and NF-kappa-B-mediated expression of proinflammatory genes. Blocks the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor-activated SMAD1-binding. Binds to regulatory elements in target promoter regions.
Subcellular Location	Nucleus.
Protein Families	Dwarfin/SMAD family
Database References	HGNC: 6772 OMIM: 602931 KEGG: hsa:4091 STRING: 9606.ENSP00000288840 UniGene: Hs.153863
Associated Diseases	Aortic valve disease 2 (AOVD2); Craniosynostosis 7 (CRS7)
Tissue Specificity	Ubiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue.

Gene Functions References

Overexpression of miR186 mimic induced HUVEC apoptosis through mitogenactivated protein kinase (MAPK) activation by targeting and inhibiting SMAD family member 6 (SMAD6). PMID: 29344637
activation of AMPK upregulated Smad6 and Smurf1 and thereby enhanced their interactions, resulting in its proteosome-dependent degradation of ALK2. PMID: 28847510
Results show that high Smad6 expression is correlated with increased risk of metastasis in ER negative breast cancer and that Smad6 determines BMP-regulated invasive behavior of breast cancer cells in a zebrafish xenograft model. PMID: 27113436
evaluated the role of inherited genetic variation in Langerhans cell histiocytosis susceptibility and identified a novel risk variant in SMAD6 PMID: 28935696
This search revealed that rare mutations that disable one copy of a gene called SMAD6 in combination with a common DNA variant near another gene called BMP2 account for about 7% of infants with midline forms of craniosynostosis. PMID: 27606499
Single Nucleotide Polymorphisms in SMAD6 gene is associated with brain metastasis in non-small-cell lung cancer PMID: 23284751
monoubiquitinated SMAD6 impairs the binding affinity of non-modified SMAD6 to the BMP type I receptor. Moreover, UBE2O and SMAD6 cooperated in the regulation of BMP7-induced adipogenesis PMID: 23455153
Smad6 indirectly maintains stemness by preventing spontaneous erythropoiesis in hematopoietic stem cells. PMID: 22705548
Loss of SMAD6 is associated with lung adenocarcinoma. PMID: 22223368
Congenital cardiovascular malformation is related to genetic variation in SMAD6 PMID: 22275001
Haplotype analysis further revealed that two haplotype blocks within SMAD6 were significantly associated with decreased ovarian cancer risk, as compared to the most common haplotype. PMID: 21984931
results suggest that MyD88 degradation driven by the Smad6-Smurf pathway is a novel mechanism for TGF-beta1-mediated negative regulation of MyD88-dependent pro-inflammatory signalling PMID: 21897371
Data show that Runx1, in conjunction with Fli1, Gata2, and Scl, directly regulates the expression of Smad6 in the aorta-gonad-mesonephros region in the developing embryo, where hematopoietic stem cells originate. PMID: 21576367
Smad6 and Smad7, inhibitors of BMP signaling, were up-regulated in HFE-mutant hereditary hemochromatosis compared to controls, disruptin bone morphogenic protein signaling. PMID: 20658468
Smad6 has no role in TGF-beta response and injury in podocytes. In contrast, Smad6 is upregulated in the mesangium in human glomerular diseases and may be involved in functions independent of TGF-beta/Smad signaling. PMID: 12397035
Smad6 and Smad7 regulate thrombomodulin-dependent activation of protein C PMID: 12407115
Smad6 repressed bone morphogenetic protein-induced Id1 transcription through recruiting transcriptional corepressor C-terminal binding protein (CtBP). PMID: 14645520
tetradecanoylphorbol-13-acetate down-regulates Smad6 expression presumably via PKCmu-ERK-dependent pathway and up-regulates Smad7 expression via PKCmu-dependent mechanism(s) which need no MAPK and NF-kappaB activation PMID: 15033458
The regulation in chondrocytes of Smad6 and Smad7 expression by IL-1beta suggests a potentially important role of IL-1beta signaling in chondrocytes, via indirect influencing of the bone morphogenetic protein/TGFbeta signaling cascade. PMID: 15529348
Adrenomedullin can attenuate TGF-beta1-mediated renal tubulointerstitial extracellular matrix turnover via an antagonistic mechanism of inhibitory Smad 6 in TGF-beta1-elicited signaling. PMID: 15665522
the level of Smad6s can alter the level of TGF-beta and the subsequent induction of PAI-1 via a FoxD1 transcription site PMID: 15716278
Smad6 and Smad7 expression affects the progression of early lesions of esophageal SCC and indicates a poor prognosis. PMID: 15736400
appears that the anti-glucocorticoid actions of Smad6 may contribute to the neuroprotective, anticatabolic and pro-wound healing properties of the TGFbeta family of proteins PMID: 16249187
Smad6 interacts with Runx2 and mediates Smad ubiquitin regulatory factor 1-induced Runx2 degradation PMID: 16299379
OAZ can alter the intensity and duration of the BMP4 stimulus through Smad6 PMID: 16373339
Smad6 appears to functionally interact with Dlx3, altering the ability of Dlx3 to bind target gene promoters. PMID: 16687405
Smad6 bound to Pellino-1 promoted TGF-beta-mediated anti-inflammatory effects. PMID: 16951688
Stimulation of Smad 6 inhibits ERK activation and thus results in a negative feedback loop to fine-tune BMP signaling in human umbilical vein endothelial cells. PMID: 17850776
A case-control study in Afro-Caribbeans found SMAD6 SNPs were not strongly associated with increased risk of developing keloids. PMID: 18445023
Splice variant Smad6B, in human prostatic and rodent testicular cell lines, exhibits a truncated C-terminus lacking the entire MH-2 domain and most parts of the linker region. PMID: 19032685
Knockdown of SMAD6 led to the activation of TGF-beta signaling through up-regulation of plasminogen activator inhibitor-1 and phosphorylation of SMAD2/3 in NSCLC. PMID: 19047146
Smad6 gene is a candidate for the genetic determinants of bone mineral density in postmenopausal women. PMID: 19277452
Study data demonstrates downregulated SMAD6 expression in psoriatic skin. PMID: 19688145

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Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

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