Recombinant Human Mucosal Addressin Cell Adhesion Molecule 1 (MADCAM1) Protein (His)

Name: Recombinant Human Mucosal Addressin Cell Adhesion Molecule 1 (MADCAM1) Protein (His)
Brand: Beta LifeScience
SKU: BLC-03796P
Price: 349.0 USD
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of this product could indicate that this peptide derived from Mammalian Cell-expressed Homo sapiens (Human) MADCAM1.

Collections: Other recombinant proteins, Recombinant proteins, Recombinant proteins holiday sale

Price Save $1,220

Size

100ug

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Product Overview

Description	Recombinant Human Mucosal Addressin Cell Adhesion Molecule 1 (MADCAM1) Protein (His) is produced by our Mammalian cell expression system. This is a extracellular protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	Q13477
Target Symbol	MADCAM1
Synonyms	Addressin mucosal; hMAdCAM 1 ; hMAdCAM-1; MACAM1; MADCA_HUMAN; MAdCAM 1; MAdCAM-1; Madcam1; Mucosal addressin cell adhesion molecule 1; Mucosal vascular addressin cell adhesion molecule 1
Species	Homo sapiens (Human)
Expression System	Mammalian cell
Tag	N-10His
Target Protein Sequence	QSLQVKPLQVEPPEPVVAVALGASRQLTCRLACADRGASVQWRGLDTSLGAVQSDTGRSVLTVRNASLSAAGTRVCVGSCGGRTFQHTVQLLVYAFPDQLTVSPAALVPGDPEVACTAHKVTPVDPNALSFSLLVGGQELEGAQALGPEVQEEEEEPQGDEDVLFRVTERWRLPPLGTPVPPALYCQATMRLPGLELSHRQAIPVLHSPTSPEPPDTTSPESPDTTSPESPDTTSQEPPDTTSPEPPDKTSPEPAPQQGSTHTPRSPGSTRTRRPEISQAGPTQGEVIPTGSSKPAGDQ
Expression Range	19-317aa
Protein Length	Extracellular Domain
Mol. Weight	34.9 kDa
Research Area	Immunology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
Subcellular Location	Membrane; Single-pass type I membrane protein.
Database References	HGNC: 6765 OMIM: 102670 KEGG: hsa:8174 STRING: 9606.ENSP00000215637 UniGene: Hs.102598
Tissue Specificity	Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ov

Gene Functions References

Both the ACT-1 assay and the MAdCAM-1 assay demonstrated acceptable reproducibility and repeatability. The assays were sufficiently stable to allow for clinical use. During clinical testing the assays demonstrated that vedolizumab was able to saturate peripheral cells at all doses tested. PMID: 25908521
Doxo-induced apoptosis has a role in inhibiting hepatocellular carcinoma by targeting Madcam1 and AKT and blocking protein translation initiation PMID: 26124182
MADCAM1 is up-regulated in liver cirrhosis with expression on peribiliary vascular plexus and lymphoid aggregates PMID: 23839340
Domain 1 of mucosal addressin cell adhesion molecule has an I1-set fold and a flexible integrin-binding loop. PMID: 23297416
Findings suggest that the inactive alpha4beta7 and alpha4beta7 activated by different stimuli have distinct conformations with different structural requirements for MAdCAM-1 binding. PMID: 21296888
Ca2+ is essential for cell tethering in flow and rolling interactions through alpha 4 integrins with MAdCAM-1 as substrate; Mg2+ promotes firm adhesion of cells on MAdCAM-1 but with much lower tethering efficiency in shear flow. PMID: 11705388
A crystal structure for the two extracellular amino-terminal domains of human MAdCAM-1 PMID: 11807247
more extensive expression of MAdCAM-1 in Crohn's disease (CrD), than in ulcerative colitis, which could contribute not only to mucosal inflammation, but also to transmural inflammation in CrD. PMID: 12100519
MAdCAM-1/integrin alpha4beta7 homing system may participate in gastric inflammation in response to H pylori-infection and contributes to MALT formation, typically leading to the development of nodular gastritis. PMID: 14669317
a marker of graft rejection in small intestine transplantation PMID: 15050155
PI3-K/Akt is involved in the gut-specific differentiation of human intestinal microvascular endothelial cells, which results in expression of the mucosal addressin MAdCAM-1 PMID: 15483224
Immobilized CCL25 and CCL28 are both able to trigger integrin alpha(4)beta(7)-dependent lymphocyte arrest on MAdCAM-1 under shear flow. PMID: 18308860
mucosal addressin cell adhesion molecule 1 may represent an important determinant for the generation of mucosal damage in celiac disease PMID: 19157500
MADCAM1 gene polymorphisms may be associated with the risk of chronic GVHD and may, also, affect mortality related to aHSCT. PMID: 19286444

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.