Recombinant Human Protein Wnt-2 (WNT2) Protein (His)

Name: Recombinant Human Protein Wnt-2 (WNT2) Protein (His)
Brand: Beta LifeScience
SKU: BLC-04799P
Price: 349.0 USD
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

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Size

100ug

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Product Overview

Description	Recombinant Human Protein Wnt-2 (WNT2) Protein (His) is produced by our Baculovirus expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	P09544
Target Symbol	WNT2
Synonyms	Int 1 related protein; Int-1-like protein 1; Int-1-related protein; INT1L 1; INT1L1; IRP; IRP protein; ONCOGENE INT1 LIKE 1; Protein Wnt-2; Secreted growth factor; Wingless type MMTV integration site family member 2; WNT 2; WNT2; WNT2_HUMAN
Species	Homo sapiens (Human)
Expression System	Baculovirus
Tag	N-10His
Target Protein Sequence	SWWYMRATGGSSRVMCDNVPGLVSSQRQLCHRHPDVMRAISQGVAEWTAECQHQFRQHRWNCNTLDRDHSLFGRVLLRSSRESAFVYAISSAGVVFAITRACSQGEVKSCSCDPKKMGSAKDSKGIFDWGGCSDNIDYGIKFARAFVDAKERKGKDARALMNLHNNRAGRKAVKRFLKQECKCHGVSGSCTLRTCWLAMADFRKTGDYLWRKYNGAIQVVMNQDGTGFTVANERFKKPTKNDLVYFENSPDYCIRDREAGSLGTAGRVCNLTSRGMDSCEVMCCGRGYDTSHVTRMTKCGCKFHWCCAVRCQDCLEALDVHTCKAPKNADWTTAT
Expression Range	26-360aa
Protein Length	Full Length of Mature Protein
Mol. Weight	41.5
Research Area	Cancer
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Functions as upstream regulator of FGF10 expression. Plays an important role in embryonic lung development. May contribute to embryonic brain development by regulating the proliferation of dopaminergic precursors and neurons.
Subcellular Location	Secreted, extracellular space, extracellular matrix. Secreted.
Protein Families	Wnt family
Database References	HGNC: 12780 OMIM: 147870 KEGG: hsa:7472 STRING: 9606.ENSP00000265441 UniGene: Hs.567356
Tissue Specificity	Expressed in brain in the thalamus, in fetal and adult lung and in placenta.

Gene Functions References

Down-regulation of miR-30a-3p/5p promotes esophageal squamous cell carcinoma cell proliferation by activating the Wnt signaling pathway through inhibition of Wnt2 and Fzd2. PMID: 29259372
The data suggest that Med19 expression correlates with aggressive characteristics of bladder cancer, and Med19 knockdown suppresses the proliferation and migration of cancer cells through down-regulating the Wnt/beta-catenin pathway. PMID: 28631286
The findings of this study have shown that molecular mechanisms, including aberrant activation of the WNT2 gene signaling pathway, may be involved in the pathogenesis of preeclampsia. PMID: 29109390
SNPs in WNT2 and FOXP2 are associated with clinical symptom severity of autism spectrum disorders. PMID: 28081867
WNT2 insufficiency might cause impaired trophoblast cell proliferation and migration via downregulation of Wnt/beta-catenin signaling pathway. PMID: 28627774
Three-dimensional organotypic co-culture assays revealed that WNT2-mediated fibroblast motility and extracellular matrix remodeling enhanced cancer cell invasion of cell lines even harboring mutations in the adenomatous polyposis coli/beta-catenin pathway. PMID: 28553956
Chondrogenic potential was higher and Wnt/beta-catenin signaling was more potently activated by a GSK-3beta inhibitor in the posterior than in the anterior part of the human infant sclera. PMID: 27336854
data enforced the evidence that knockdown of c-Fos inhibited cell proliferation, migration, and invasion, and promoted the apoptosis of OS cells accompanied by altered expression of Wnt2 and Fzd9 PMID: 28665975
High WNT2 expression is associated with Cervical Carcinoma Metastasis. PMID: 27513465
targeting beta-catenin with lipofection-delivered small interfering RNA oligonucleotide inhibited the proliferation and cell cycle arrest in G0/G1 phase and increased apoptosis of fibroblast cells, accompanied by downregulation of Wnt2 and cyclin D1 as well as the phosphorylation level of glycogen synthase kinase 3 beta in the keloid fibrosis PMID: 28656880
Genetic polymorphisms in the WNT2 and CTNNB1 genes were closely associated with hepatocellular carcinoma risk and progression in a Chinese Han population PMID: 28328801
The WNT2 rs39315 G allele was associated with advanced tumor stage in hepatocellular carcinoma. PMID: 26968103
Wnt2 complements Wnt/beta-catenin signaling in regulating colorectal cancer cell proliferation. PMID: 26484565
Wnt2/beta-catenin signaling was involved in stromal-epithelial cells interaction in endometriosis. PMID: 26116230
High expression of Wnt2 is associated with pancreatic cancer progression promoted by pancreatic stellate cells. PMID: 25731618
Hypermethylation of WNT2 is associated with colorectal cancer. PMID: 23694962
The study demonistrated that expressions of Wnt-2 in high-grade brainstem gliomas were significantly higher than that in low-grade brainstem gliomas and normal brain tissues and were positively correlated with the expression of Ki-67. PMID: 23603171
Decreased placental expression of Wnt2 and increased placental expression of sFRP4 may be associated with the pathogenesis of severe preeclampsia. PMID: 23322712
We demonstrated an activation of Wnt-2 signaling via the Frizzled-8 receptor in non-small cell lung cancer cells PMID: 23815780
The skin lesions of scleroderma patients showed obviously increased expression of cytoplasmic Wnt 2, nuclear Wnt 3a and beta-catenin, but markedly lowered cell membrane expression of beta-catenin than normal skin. PMID: 23268410
significant association with SNP rs4730775 locus on chromosome 7 with genetic susceptibility to Peyronie's disease PMID: 22489561
Pdgf signaling potentiates Wnt2-Wnt7b signaling to promote high levels of Wnt activity in mesenchymal progenitors that is required for proper development of endoderm-derived organs, such as the lung PMID: 22949635
The WNT2 gene may play a suggestive role in language development in autistic disorder. PMID: 22522212
Expression of WNT2 in pancreatic cancer cells suppresses anoikis, enhances anchorage-independent sphere formation, and increases metastatic propensity in vivo. PMID: 22763454
Wnt/beta-catenin pathway forms a negative feedback loop during TGF-beta1 induced human normal skin fibroblast-to-myofibroblast transition PMID: 22041457
a haplotype of WNT2 (rs2896218-rs6950765: G-G) is significantly associated with ASDs. PMID: 21575668
DNA methylation within the WNT2 promoter in human placenta is associated with low birthweight. PMID: 21474991
findings provide evidence for a significant association between WNT2 and autism. PMID: 19895723
The present study does not support a major role for WNT2 in schizophrenia. PMID: 20492734
Wnt2/beta-catenin signaling pathway is constitutively activated in esophageal squamous cell carcinoma (ESCC) cells, indicating that Wnt2/beta-catenin pathway plays an essential role in carcinogenesis of the esophagus. PMID: 19857041
Wnt2 acts as an angiogenic factor for non-sinusoidal endothelium in vitro and in vivo. PMID: 19444628
Up-regulation of WNT2 mRNA by estrogen might play a key role in some cases of human breast cancer through activation of the beta-catenin - TCF signaling pathway. PMID: 11712082
Activating mutations of the WNT2 gene are not a major contributor to the development of autistic disorder. PMID: 11840514
REVIEW: role in human gastrointestinal cancer PMID: 14533014
Our interpretation of these findings is that it is unlikely that DNA variations in RELN and WNT2 play a significant role in the genetic predisposition to autism. PMID: 15048648
Target genes involved in tumor WNT pathways in response to TGF-beta1 were found in cervix cancer cells. PMID: 15878915
Wnt/beta-catenin signalling pathway is activated in most of gastric cancers, which may play pivotal roles either in gastric cancer formation or in tumour invasion and dissemination PMID: 15896469
Inhibition of Wnt2 by monoclonal antibodies is of therapeutic interest in the development of more effective treatments for malignnt pleueral mesothelioma. PMID: 15900580
The increased Wnt2 expression in gastric cancers is paralleled with reduction of membranous E-cadherin. PMID: 16132582
The WNT2 gene is upregulated along the progression from low-grade dysplasia to esophageal adenocarcioma. PMID: 16407829
Regulation of Fz4 expression by Wnt2. PMID: 17386109
inhibition of both Wnt-2 and Gal-3 had synergistic effects on suppressing canonical Wnt signaling and inducing apoptosis, suggesting that aberrant canonical Wnt/beta-catenin signaling in colorectal cancer can be regulated at multiple levels PMID: 17534895
potential coordinative effects of Wnt, NF-kappaB and/or Stat3 activation on gastric cancer formation presumably by promoting the transcription of their common target genes. PMID: 18184402
Persistent upregulation in colorectal carcinoma cases studied. PMID: 18600204
Wnt (Wnt2), Stat3, and Notch-1 and -2 signaling are correlated in human epidermal tumors. PMID: 18703315
Knockdown of Wnt2 by siRNA in human U251 glioma cells inhibited cell proliferation and invasive ability, and induced apoptotic cell death PMID: 18949017
The results suggest that WNT2 could act through its receptor FZD9 to regulate the beta-CATENIN pathway in cumulus cells, recruiting beta-CATENIN into plasma membranes and promoting the formation of adherens junctions involving CDH1. PMID: 19038973
up-regulation of the Wnt-2 protein might play a role in the development of colorectal cancers. PMID: 19239325
RNA samples from 21 neuroblastoma showed a highly significant FZD1 and/or MDR1 overexpression after treatment, underlining a role for FZD1-mediated Wnt/beta-catenin pathway in clinical chemoresistance. PMID: 19421142

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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