Recombinant Human PSG1 Protein (C-6His)

Name: Recombinant Human PSG1 Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-0756NP
Availability: InStock

BL-0756NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Description	Recombinant Human Pregnancy-specific beta-1-glycoprotein 1 is produced by our Mammalian expression system and the target gene encoding Gln35-Pro419 is expressed with a 6His tag at the C-terminus.
Accession	P11464
Synonym	Pregnancy-specific beta-1-glycoprotein 1;PSBG-1;CD66 antigen-like family member F; Fetal liver non-specific cross-reactive antigen 1/2; PSG95; Pregnancy-specific beta-1 glycoprotein C/D
Gene Background	Pregnancy-specific beta-1-glycoprotein 1(PSBG-1 for short), also named CD66 antigen-like family member F, Fetal liver non-specific cross-reactive antigen 1/2, PSG95, Pregnancy-specific beta-1 glycoprotein C/D, is a secreted protein which belongs to the immunoglobulin superfamily, CEA family. It contains 3 Ig-like C2-type (immunoglobulin-like) domains and 1 Ig-like V-type (immunoglobulin-like) domain. The human placenta is a multihormonal endocrine organ that produces hormones, enzymes, and other molecules that support fetal survival and development.
Molecular Mass	44.5 KDa
Apmol Mass	68 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Subcellular Location	Secreted.
Protein Families	Immunoglobulin superfamily, CEA family
Database References	HGNC: 9514 OMIM: 176390 KEGG: hsa:5669 STRING: 9606.ENSP00000244296 UniGene: Hs.709192

Gene Functions References

Inhibiting PSG1 can effectively reduce chemoresistance via a transforming growth factor-beta (TGF-beta)-related pathway in ER-negative breast cancer cells. PMID: 27653744
PSG stimulated IDO activity under the conditions of induction of the monocytes by interferon-gamma. PMID: 27595833
Under conditions of directed induction of phenotypic changes in CD4(+) lymphocytes to regulatory T cells (Treg), pregnancy-specific at high concentrations (10 and 100 mug/mL) increased FOXP3 expression. The evaluation of the spontaneous expression level of FOXP3 mRNA showed that pregnancy-specific beta1-glycoprotein (1 and 100 mug/mL) stimulated the expression of this factor in mononuclear cells and isolated CD4(+) lym... PMID: 27817017
results identify a non-placental site of PSG expression in the gut and associated tumors, with implications for determining whether PSGs have a role in tumor progression, and utility as tumor biomarkers. PMID: 26926266
the presence of GAGs on the surface of endothelial cells was required for the ability of PSG1 to induce tube formation PMID: 21193412
The aspartic acid at position 95 is not required for PSG1 activity but the amino acids implicated in the formation of a salt bridge within the N-domain are essential. PMID: 20335639
first trimester maternal serum marker of small-for-gestational age and preterm delivery PMID: 19911417
Human PSG1a induces alternative activation in human & mouse monocytes & suppresses the accessory-cell-dependent T-cell proliferation. PSG1a contributes to immunomodulation during pregnancy, with opposite effects on maternal innate & adaptative systems. PMID: 12223519
The differential expression of HLA-E and PSG1 in the sperm of fertile and infertile men is discussed. PMID: 18987160

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.