Recombinant Human SCF Protein (aa 1-189, His Tag)

Name: Recombinant Human SCF Protein (aa 1-189, His Tag)
Brand: Beta LifeScience
SKU: BLPSN-4156
Availability: InStock

Collections: Cytokines and growth factors, Growth factors and receptors, Recombinant proteins, Recombinant stem cell factor (scf) proteins

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Product Overview

Tag	His
Host Species	Human
Accession	P21583
Synonym	C-kit ligand, DCUA, FPH2, FPHH, Kitl, KL-1, MGF, SCF, SF, SHEP7
Background	Similar to Kit ligand precursor (C-kit ligand) , also known as Stem cell factor (SCF), Mast cell growth factor (MGF) or Hematopoietic growth factor KL. SCF/C-kit ligand is the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. SCF/C-kit ligand stimulates the proliferation of mast cells. This protein is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It may act synergistically with other cytokines, probably interleukins SCF/C-kit ligand is the ligand for the tyrosine kinase receptor c-kit, which is expressed on both primitive and mature hematopoietic progenitor cells. In vitro, SCF/C-kit ligand synergizes with other growth factors, such as granulocyte colony-stimulating factor (G-CSF), granulocyte macrophage- colony- stimulating factor, and interleukin-3 to stimulate the proliferation and differentiation of cells of the lymphoid, myeloid, erythroid, and megakaryocytic lineages. In vivo, SCF/C-kit also synergizes with other growth factors and has been shown to enhance the mobilization of peripheral blood progenitor cells in combination with G-CSF. In phase I/II clinical studies administration of the combination of SCF and G-CSF resulted in a two- to threefold increase in cells that express the CD34 antigen compared with G-CSF alone.
Description	A DNA sequence encoding the amino acid (Met 1-Ala 189) of human SCF (P21583-1) extracellular domain was expressed, with a His tag at the C-terminus.
Source	Baculovirus-Insect Cells
Predicted N Terminal	Glu 26
AA Sequence	Met 1-Ala 189
Molecular Weight	The secreted recombinant human SCF (aa 1-189) consists of 175 a.a. and predicts a molecular mass of 19.9 kDa. The apparent molecular mass of rh SCF is approximately 22 kDa in SDS-PAGE under reducing conditions.
Purity	>92% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.The ED50 for this effect is 2-8 ng/mL.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
Subcellular Location	[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane; Single-pass type I membrane protein. Cell projection, lamellipodium. Cell projection, filopodium.; [Soluble KIT ligand]: Secreted.
Protein Families	SCF family
Database References	HGNC: 6343 OMIM: 145250 KEGG: hsa:4254 STRING: 9606.ENSP00000228280 UniGene: Hs.1048
Associated Diseases	Hyperpigmentation with or without hypopigmentation, familial progressive (FPHH); Deafness, congenital, unilateral or asymmetric (DCUA)

Gene Functions References

Authors show here that cyclin F, a substrate receptor F-box protein for the SCF (Skp1/Cul1/F-box) family of E3 ubiquitin ligases, is a bona fide AKT substrate. PMID: 28954236
A statistically significant relationship was found between stem cell factor level and oocyte maturation, embryo quality, and clinical pregnancy. PMID: 28285567
PAR2 plays a direct role in melanogenesis by increasing SCF secretion from keratinocytes. PMID: 26909822
We did not find any KITLG mutation in two affected individuals with familial progressive hyperpigmentation and hypopigmentation. PMID: 27859606
results suggest that SCF can accelerate cell homing and the maturation of the pulp-dentin complex in human immature teeth. PMID: 27762658
SCF is a critical regulatory factor for conceptus development and implantation during pregnancy in pigs. PMID: 28551414
results revealed that the Th17-mediated inflammatory environment promotes mast cell accumulation through keratinocyte-derived SCF PMID: 28456630
Bone marrow adipocytes synthesize SCF, promoting hematopoietic stem cell proliferation/regeneration. PMID: 28714970
Hypoxia microenvironment in hepatocellular carcinoma up-regulates HIF2/SCF expression, which in turn promotes angiogenesis and HCC metastasis. PMID: 28153790
Insights into the transmembrane helix associations of Kit ligand have been reported. PMID: 28370370
There were no significant differences in CD334-SCF double-positive FB and CD31-SCF double-positive EC levels between the two periapical tissue groups (P > 0.05). CD14-SCF double-positive MP density was considerably higher in periapical granulomas than in cysts (P < 0.01). PMID: 28290616
A significant association of rs995030 of the KITLG gene with the development of testicular germ cell tumors (p = 0.029 for the allele G, p = 0.0124 for the genotype GG) was revealed. PMID: 28064312
Overexpression of SCF is associated with Lewis lung carcinoma. PMID: 26666817
both SCF and IL-31 play an important role in mediating inflammation and enhancing severity of atopic asthma. PMID: 26853551
women with endometriosis have low levels of endometrial SCF PMID: 26791471
results suggested the existence of E2F1-miR-34c-SCF negative feedback loop which was interrupted by the hyper-methylation of miR-34c promoter in CRC cells and increased cell proliferation. PMID: 26704889
IL-1b, which normally bridges innate and adaptive immunity, induces the production of the major haematopoietic/proleukaemic growth factor SCF through the PI-3K/mTOR pathway and the HIF-1a transcription complex. PMID: 25418470
We identified a new heterozygous missense mutation in two families with hyper- and hypopigmentation disorders (p.Thr34Asn and p.Val37Gly, respectively). Of the six affected individuals examined by us, two had cancer. PMID: 26179221
Na+/H+ antiporter inhibition induces cell death in TF-1 erythroleukemia cells stimulated by the stem cell factor PMID: 26188365
IL-7 and SCF are elevated for a prolonged period after double umbilical cord blood transplantation and persistently high levels of these cytokines may correlate with worse clinical outcomes. PMID: 26177551
Allelic Mutations of KITLG, Encoding KIT Ligand, Cause Asymmetric and Unilateral Hearing Loss and Waardenburg Syndrome Type 2. PMID: 26522471
Foxc1 is essential for progenitor cell development and maintenance of bone marrow niches for these cells upregulating CXCL12 and SCF expression and inhibiting of adipogenic processes in cell progenitors. (Review) PMID: 26049754
Results identified SCF as a direct target of HIF-1 in pancreatic cancer. SCF/c-kit promoted invasion and proliferation of pancreatic cancer cells and may be a new target for the treatment of pancreatic cancer. PMID: 25799412
we found that Kl treatment impairs Nodal mRNA expression and Fgf9-mediated Nanos2 induction, reinforcing the antagonistic effect of these two growth factors on the meiotic fate of male germ cells PMID: 25766327
A functional p53 responsive polymorphism in KITLG, rs4590952, does not affect the risk of breast cancer. PMID: 25220285
High SCF expression is associated with metastasis in non-small cell lung cancers. PMID: 25401222
SCF-mediated gamma-globin gene expression in adult human erythroid cells is associated with KLF1, BCL11A and SOX6 down-regulation. PMID: 25457384
overexpression of Snail in PDAC cells increased SCF levels, and the media conditioned by Snail-expressing PDAC cells promoted mast cell migration. PMID: 24944064
Paraffin-embedded, formalin-fixed tissue from 100 consecutive patients with invasive breast cancer was immunohistochemically stained for Ki-67 and Ki-67/KL1. Ki-67 was manually scored in different regions by 2 observers and automated image analysis PMID: 24897070
KIT-L could be now a direct NOBOX target. PMID: 25514101
Stem cell factor is a novel independent prognostic biomarker for hepatocellular carcinoma after curative resection. PMID: 25086759
Together, these data indicate that stem cell factor1-165 is more thermostable than stem cell factor1-141. PMID: 25061857
High KITLG is associated with growth and invasion in colorectal cancer. PMID: 25213795
These findings suggest that integration of SCF and LTD4 signals may contribute to MC hyperplasia and hyper-reactivity during airway hyper-response and inflammation PMID: 25161061
study reports a modulatory role for antimullerian hormone as an ovarian/follicular autocrine/paracrine factor controlling SCF expression via the cAMP/PKA pathway in granulosa cells PMID: 25241372
Gain-of-function mutations in c-Kit result mastocytosis, gastrointestinal stromal tumors and other hematological neoplasms. (Review) PMID: 24745671
Variants of the KITLG enhancer is associated with blond hair color. PMID: 24880339
Data reveal that SCF disrupts the endothelial adherens junction and enhances vascular leakage, and suggest that anti-SCF/cKit therapy may hold promise as a potential therapy for the treatment of hyperpermeable vascular diseases. PMID: 24790137
Data indicate that insulin-like growth factor-1 (IGF1) stimulated s tem cell factor (Kitl/KITLG) protein and mRNA expression and promoter activity by activating several signaling pathways. PMID: 24116170
Cardiomyocyte-specific overexpression of SCF protects the heart from myocardial ischemia reperfusion injury. PMID: 23680593
Data indicate that stem cell factor (SCF) was expressed in differentiating dental pulp. PMID: 23703692
Seborrheic keratosis cells do not respond to IL-1alpha or TNF-alpha to stimulate production of SCF, whereas a significant stimulation of SCF is elicited by those same cytokines in normal human keratinocytes. PMID: 23662587
Study uncovered a single-nucleotide polymorphism in a functional p53-binding site and establish its influence on the ability of p53 to bind to and regulate transcription of the KITLG gene. The SNP resides in KITLG and associates with one of the largest risks identified among cancer genome-wide association studies. PMID: 24120139
N-linked carbohydrate modifiers affect stem cell factor and endothelin-1-stimulated epidermal pigmentation in human epidermal equivalents PMID: 23273644
RIN3 is a negative regulator of mast cell responses to SCF PMID: 23185384
this is the first report to determine the significant contribution of SCF autoproduction to neoplastic proliferation of mast cells PMID: 23125456
A unique requirement for KIT Ligand in the upregulation of glucocorticoid receptor alpha isoform and optimal erythroblast expansion in cultures that mimic stress erythropoiesis. PMID: 22533504
Stem cell factor was expressed in all tumour subgroups, with the highest in oncocytomas and papillary renal cell carcinomas. Stem cell factor correlated positively with the cytoplasmic expression of KIT. PMID: 23060556
Elevated plasma levels of SCF are associated with inflammatory bowel disease with JCV infection. PMID: 22467521
The KITLG (rs4474514) variants were significantly associated with germ cell tumors only in the adolescent age group. PMID: 22072546

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