Recombinant Human SNAP-25 Protein (His Tag)

Name: Recombinant Human SNAP-25 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-4303
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	His
Host Species	Human
Accession	P60880
Synonym	bA416N4.2, dJ1068F16.2, RIC-4, RIC4, SEC9, SNAP, SNAP-25
Background	Synaptosomal-associated protein 25, also known as Super protein, Synaptosomal-associated 25 kDa protein, SNAP25 and SNAP, is a cytoplasm and cell membrane protein which belongs to theSNAP-25 family. SNAP25 / SUP contains 2t-SNARE coiled-coil homology domains. SNAP25 / SUP is a membrane bound protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP25 / SUP protein is a component of the SNARE complex, which is proposed to account for the specificity of membrane fusion and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together. SNAP25 / SUP is a Q-SNARE protein contributing two alpha-helices in the formation of the exocytotic fusion complex in neurons where it assembles with syntaxin-1 and synaptobrevin. SNAP25 / SUP is involved in the molecular regulation of neurotransmitter release. It may play an important role in the synaptic function of specific neuronal systems. SNAP25 / SUP associates with proteins involved in vesicle docking and membrane fusion. SNAP25 / SUP regulates plasma membrane recycling through its interaction with CENPF. SNAP25 / SUP inhibits P/Q- and L-type voltage-gated calcium channels located presynaptically and interacts with the synaptotagmin C2B domain in Ca2+-independent fashion. In glutamatergic synapses SNAP25 / SUP decreases the Ca2+ responsiveness, while it is naturally absent in GABAergic synapses.
Description	A DNA sequence encoding the human SNAP25 (P60880-1) (Met 1-Gly 206) was expressed, with a His tag at the N-terminus.
Source	E.coli
Predicted N Terminal	Met
AA Sequence	Met 1-Gly 206
Molecular Weight	The recombinant human SNAP25 consisting of 217 a.a. and has a calculated molecular mass of 24.8 kDa. It migrates as an approximately 28 kDa band in SDS-PAGE under reducing conditions.
Purity	>90% as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.
Subcellular Location	Cytoplasm, perinuclear region. Cell membrane; Lipid-anchor. Cell junction, synapse, synaptosome. Photoreceptor inner segment.
Protein Families	SNAP-25 family
Database References	HGNC: 11132 OMIM: 600322 KEGG: hsa:6616 STRING: 9606.ENSP00000254976 UniGene: Hs.167317
Associated Diseases	Myasthenic syndrome, congenital, 18 (CMS18)
Tissue Specificity	Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.

Gene Functions References

Comparing clinical features of reported patients with SNAP25 mutations, the current patients demonstrated apparently milder clinical features with normal intelligence, and no magnetic resonance imaging abnormality or facial dysmorphism. Our results expand the clinical spectrum of SNAP25 mutations. PMID: 29491473
report some evidence supporting the association of SNAP25 to Attention Deficit/Hyperactivity Disorder PMID: 26941099
Single nucleotide polymorphism in SNAP-25 gene is associated with Attention deficit hyperactivity disorder. PMID: 28512748
Findings indicate that lower SNAP-25 in ventromedial caudate in schizophrenia is primarily due to less SNAP-25A, and that the remaining SNAP-25 is associated with greater protein-protein interaction with syntaxin. The absence of a link to recent treatment effects in the human samples, or to administration of antipsychotic drugs in rats suggests the findings are illness- and not treatment-related. PMID: 26971072
Allelic variation of SNAP25 modulates development and plasticity of the prefrontal-limbic network and a shared genetic vulnerability between Bipolar Disorder and Schizophrenia. PMID: 28972123
SH3BP5, LMO3, and SNAP25 were expressed in diffuse large B-cell lymphoma cells and associated with clinical features. PMID: 27184832
show that FOXC1 regulates the expression of RAB3GAP1, RAB3GAP2 and SNAP25 PMID: 28575017
The results of this study suggested that the polymorphisms rs3746544 and rs1051312 may increase the odds of developing ADHD. PMID: 27380186
study demonstrated that miR-27a and -b, which are widely expressed in host cells, suppress SNAP25 and TXN2 expression through posttranscriptional gene silencing. PMID: 28356525
Data suggest that A-syn (alpha-synuclein) promotes SNARE-dependent vesicle docking; phosphatidylserine (PS) removal from t-SNARE-bearing vesicles causes A-syn to inhibit vesicle docking; PS removal from v-SNARE-bearing vesicles promotes vesicle docking; the C-terminal 45 residues of A-syn are required for promotion of vesicle docking. (Here, t-SNARE is SNAP-25; v-SNARE is VAMP2.) PMID: 28495859
our data indicate that the expression of SNAP25 is crucial for dendrite formation and is associated with the effects of targeted chemotherapy. The detection of SNAP25 expression in MB cells may thus be essential for the chemotherapeutic application of Ara-C. PMID: 28339008
Robust association of the rs3746544 SNP and ASD, in both allele and haplotype-based analyses, was observed in Iranian population. PMID: 27888397
Our results will provide novel evidence to reveal the possible role of SNAP-25 in B[a]P-induced neurotoxicity and may be helpful for searching the potential strategy for the prevention measures against B[a]P neurotoxicity. PMID: 28412278
Our analysis indicated that there is no significant association between none of studied variants in SNAP-25 and ADHD. PMID: 27627841
These results of this study suggested that SNAP25 and NOS1 genotypes influence ADHD symptoms only in adults with ADHD. PMID: 26821215
Snap25 rs363050 (G) allele, which results in a reduced expression of Snap25, is associated with altered glycemic parameters in T2DM possibly because of reduced functionality in the exocytotic machinery leading to suboptimal release of insulin. PMID: 26779543
single nucleotide polymorphisms in either the region of NEUROD6 or SNAP25 were significantly associated with Alzheimer's Disease, in APOE4+ females and APOE4+ males, respectively. PMID: 26395074
Data demonstrate a role for SNAP-25 in controlling PSD-95 clustering and open the possibility that genetic reductions of the protein levels may contribute to the pathology through an effect on postsynaptic function and plasticity. PMID: 25678324
BoNT-A injection, but not Lipotoxin instillation, effectively cleaves SNAP-25 in the suburothelium. PMID: 26241848
The expression of SNAP-25 within the enteric nervous system and its downregulation in DD suggest an essential role in enteric neurotransmission and as a marker for impaired synaptic plasticity in enteric neuropathies. PMID: 25655772
The SNAP-25 Ddel T/C genotype was more common in fibromyalgia syndrome patients compared to controls, and it related to behavioral symptoms, personality and psychological disorders. PMID: 24885975
The rs363050 gene polymorphism correlates with decreasing cognitive scores in autistic children. PMID: 25629685
This study demonstrated that SNAP-25 polymorphisms may be associated with Alzheimer's disease and correlate with alterations in categorical fluency and a reduced localized brain activity. PMID: 25024311
SNARE complex genes and their interactions may play a significant role in susceptibility and working memory of ADHD. PMID: 25445064
Patterns of the immunoreactivity with antibodies to SNAP-25, synapsin-I and synaptophysin are completely appropriate to those of adult's OB on the 38-40 weeks of the prenatal development. PMID: 26204769
The results show that the positively charged amino acids at the SNAP-25 C terminus promote tight SNARE complex zippering and are required for high release frequency and rapid release in individual fusion events. PMID: 25698757
Ile67Asn variant in SNAP25B is pathogenic because it inhibits synaptic vesicle exocytosis PMID: 25381298
Cerebrospinal fluid SNAP-25 differentiated Alzheimer's disease from controls PMID: 25418885
SNPs in SNAP25 represent a common risk factor of both schizophrenia and major depressive disorder in the Han Chinese population. PMID: 25650683
In conclusion,the present results of a family-based association study do not support a strong role of SNARE genes in adult ADHD PMID: 24176595
Full-length SNAP25 is cleaved more efficiently by the protease domain of serotype A botulinum neurotoxin than its shorter fragments. PMID: 24769566
found evidence of the association of SNAP25 and ADHD. PMID: 24362847
no associaton with idiopathic generalized epilepsy was found regarding Intron 7 rs1569061 of Syntaxin 1A gene, MnlI rs3746544 and DdeI rs1051312 polymorphisms of SNAP-25 gene compared with healthy subjects PMID: 24164654
Association of impulsivity and polymorphic microRNA-641 target sites in the SNAP-25 gene. PMID: 24391914
direct interaction between TRIM9 and the Netrin-1 receptor DCC as well as a Netrin-1-sensitive interaction between TRIM9 and the SNARE component SNAP25. The interaction with SNAP25 negatively regulates SNARE-mediated exocytosis and axon PMID: 24778312
A combination of NET1 (rs2242447) and SNAP-25 (rs3746544) is a risk factor for ADHD. PMID: 23872233
DNA variation at SNAP25 confers risk to attention deficit disorder with hyperactivity. PMID: 23593184
Significant associations between two SNPs in the SNAP-25 gene (rs363039 and rs363050) and shyness was found. PMID: 23888754
There was an association between schizophrenia and the SNAP-25 rs1503112 polymorphism found, which survived correction for multiple testing. PMID: 22940547
There was a trend towards the increase in the frequency of an G allele of SNAP-25 in siblings of patients. PMID: 23612411
syntaxin 1 and SNAP-25 cooperate as SNARE proteins to support neuron survival. PMID: 23403573
PRIP inhibits regulated exocytosis through the interaction of its C2 domain with syntaxin 1 and SNAP-25, potentially competing with accessory proteins such as synaptotagmin I and by directly inhibiting trans-SNARE complex formation PMID: 23341457
low IQ group of children has higher frequency of SNAP25 allele, associated with intellectual disability. PMID: 22762387
There was a loss of SNAP-25 in visual cortex in dementia with Lewy bodies compared to controls. PMID: 23242284
no Alzheimer's Disease-associated differences in SNAP25 promoter DNA methylation were observed PMID: 22732502
Data obtained in this preliminary study indicates that rs3746544 'T' SNAP25 allele may have some role in the disease etiology in the studied Indian population PMID: 21996783
SNAP25 might represent the first description of an adaptively evolving gene with a role in cognition. PMID: 22193912
Letter: incubation of pancreatic islets with fibronectin improves beta cell function via increased expression of syntaxin 1 and SNAP25. PMID: 21926557
Forming an acceptor SNARE complex between syntaxin-1A and SNAP-25 weakens but does not abrogate cholesterol-controlled cluster formation and indicates that the reconstitution process results in equal incorporation of protein at either lipid composition. PMID: 21916482
DdeI and MnII T/T genotypes of SNAP25 may be a risk factor for antisocial behavior in a Turkish population. PMID: 21756448

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