Recombinant Human STC-1 Protein (C-6His)

Name: Recombinant Human STC-1 Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-2328NP
Availability: InStock

BL-2328NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Description	Recombinant Human Stanniocalcin 1 is produced by our Mammalian expression system and the target gene encoding Thr18-Ala247 is expressed with a 6His tag at the C-terminus.
Accession	P52823
Synonym	Stanniocalcin 1; stanniocalcin-1; STC1; STC-1; STCSTC-1
Gene Background	Stanniocalcin 1 (STC-1) is a homodimeric glycoprotein hormone that is involved in calcium and phosphate homeostasis. It was originally identified in bony fishes, where elevation of calcium in serum causes the release of STC from the endocrine glands called the corpuscles of Stannius. STC-1 inhibits the breakdown of PAPP-A, protects cancer cells from apoptosis, reduces tumor size of liver cancers, promotes osteoblast differentiation and inhibits longitudinal bone growth directly at the growth plate. It is also a biomarker of brain and lung cancer progression. STC1 signals through inhibitory G-protein modulates CGRP receptor spatial localization during osteoblastogenesis and may function as a regulatory factor interacting with calcitonin peptide members during bone formation.
Molecular Mass	26.9 KDa
Apmol Mass	28-36 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Stimulates renal phosphate reabsorption, and could therefore prevent hypercalcemia.
Subcellular Location	Secreted.
Protein Families	Stanniocalcin family
Database References	HGNC: 11373 OMIM: 601185 KEGG: hsa:6781 STRING: 9606.ENSP00000290271 UniGene: Hs.25590
Tissue Specificity	Expressed in most tissues, with the highest levels in ovary, prostate, heart, kidney and thyroid. In the kidney, expression is confined to the nephron, specifically in the distal convoluted tubule and in the collecting tubule. Not detected in the brain, l

Gene Functions References

these results suggest that mesenchymal stem cells protect vascular cells from inflammatory injury partially by secreting STC1 PMID: 29857240
Data indicate that stanniocalcin 1 (STC1) is a non-canonical NOTCH1 ligand and acts as a crucial regulator of stemness in glioblastoma (GBM). PMID: 29196129
these data demonstrated that STC1 can promote cell apoptosis via NF-kappaB phospho-P65 (Ser536) by PI3K/AKT, IkappaBalpha and IKK signaling in cervical cancer cells PMID: 28545028
Ssecretory STC1 enhances metastatic potential of hepatocellular carcinomas via JNK signaling. PMID: 28688970
Glycolysis from glucose is regulated by hSTC-1, decreasing the adequate supply of glycerol-3-phosphate (G3P) needed for fatty acid esterification and triacylglycerol (TG) storage in brown adipose tissue (BAT). The decrease of TG capacity synthesis from glucose by hSTC-1 compromises the BAT thermogenic capacity. PMID: 28435144
Increased STC1 plasma level represents a hallmark of late-onset preeclampsia; STC1 gene variants modulate placental gene expression and maternal hormone levels. PMID: 27603899
Latanoprost-induced reduction of intraocular pressure is mediated through the downstream signaling molecule STC-1. When used by itself, STC-1 exhibits ocular hypotensive properties. PMID: 28538979
Study demonstrates that STC1 is overexpressed in the prostate carcinoma cell lines and suggests that it promotes prostate carcinoma cell proliferation via cyclin E1/CDK2. PMID: 28350121
In the fed state, STC1 increases the incorporation of (14)C from glucose into lipids in the white retroperitoneal adipose tissue (WRAT). STC1 is one of the hormonal factors that control glucose metabolism in WRAT in the fed state. PMID: 27730346
STC1 protein is significantly up-regulated in midsecretory endometrial fluid and is dysregulated in eutopic endometrial tissue from women with endometriosis. It is likely regulated by cAMP and may be involved in the pathogenesis of decidualization defects. PMID: 27322879
STC1 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
elevated STC-1 expression is associated with poor clinical outcome in triple-negative breast cancer (TNBC) patients, and STC-1 is directly involved in the invasiveness of TNBC cells PMID: 27461417
we demonstrated that aberrant STC1 expression is associated with poor prognosis and stimulates the invasiveness of triplenegative breast cancer cells PMID: 27459971
our findings strongly suggest that elevated expression of STC1 protein at the III-IV stage of lung adenocarcinoma promotes tumorigenesis of lung adenocarcinoma and positively associates with the cancer progression, which may be of potential value as tumor marker in clinical tracking lung adenocarcinoma progression. PMID: 26577859
Data suggest that stanniocalcin 1 and 2 (STC1, STC2) participate in inhibition of proteolytic activity of pregnancy-associated plasma protein-A (PAPP-A) during folliculogenesis. PMID: 26874357
STC1 gene expression at diagnosis might be a useful prognostic marker for clinical outcome and monitoring therapeutic response in patients with acute leukemia. PMID: 26547904
STC1 blunts bleomycin-induced rise in thrombin protein and activity, diminishes thrombin-induced signaling through PAR1 to ERK, and inhibits bleomycin-induced pneumonitis. PMID: 26640170
Data revealed the existence of a moderating effect between Klotho and STC1, where Klotho may inhibit thyroid tumor progression by inhibiting the tumor marker level of STC1. PMID: 26531219
High expression levels of stanniocalcin-1 is associated with Hepatocellular Carcinoma. PMID: 26469082
Results show that STC1 have an important role in the carbohydrate metabolism regulation, in particular gluconeogenesis from glutamine in the kidney, across the vertebrates. PMID: 26187698
Stanniocalcin 1 is expressed in thyroid side population cells and thyroid cancer cells. PMID: 25647164
Data show that co-transfection with cDNA encoding stanniocalcin-1 abrogates the proteolytic activity of pregnancy-associated plasma protein-A (PAPP-A) toward IGF-binding protein 4 (IGFBP-4). PMID: 26195635
These findings demonstrate a role for STC1 in metastasis of early stage clear cell renal cell carcinoma. PMID: 25740019
Mesenchymal stem cells correct inappropriate epithelial-mesenchyme relation in pulmonary fibrosis using Stc1. PMID: 25373521
These results suggested that STC1 may be a valuable biomarker in diagnosing malignant degree of glioma and evaluating prognostic following surgery PMID: 25783529
STC1 interferes with CALCRL signaling during osteoblastogenesis via adenylate cyclase inhibition. PMID: 25591908
Stanniocalcin 1 downregulation is responsible for sorafenib-induced cardiotoxicity through activated ROS generation. PMID: 25370841
Circulating STC1 and STC2 mRNA are potentially useful blood markers for LSCC PMID: 24743310
This is the first study to show definitively that STC1 plays an oncogenic role in breast cancer, and indicates that STC1 could be a potential therapeutic target for treatment of breast cancer patients. PMID: 25391215
High STC1 expression is associated with glioma. PMID: 24729417
was indicated that secreted STC-1 promotes metastatic potential of breast cancer cells via activation of PI3K/AKT PMID: 25056605
Stanniocalcin-1 and -2 promote angiogenic sprouting in HUVECs via VEGF/VEGFR2 and angiopoietin signaling pathways. PMID: 23664860
An anti-apoptotic protein stanniocalcin-1 (STC-1) can prevent loss of retinal ganglion cells in the rat retina with optic nerve transection PMID: 23667669
Data of this experiment showed that higher STC-1 expression during 96 h of preconditioning correlated with more effective MMP protection through the regulation of intracellular level of ROS and cytosolic free Ca2 + and preservation of cell viability. PMID: 23566487
STC1 is differentially expressed in the culprit coronary plaques of patients with acute myocardial infarction versus those with stable angina. STC1 may play a role in plaque instability. PMID: 23757035
Thus, these results provided evidence that STC1 inhibited cell proliferation and invasion through NF-kappaB p65 activation in cervical cancer. PMID: 23382863
Data indicate that the secreted glycoprotein stanniocalcin-1 (STC1) as a mediator of metastasis by PDGF receptor function in the setting of colorectal cancer. PMID: 23243022
STC-1 mRNA expression is a reliable marker for detection of DTCs in PB and BM of ESCC patients, and STC-1-positive DTCs may be a promising tool for diagnosis and prognosis assessment in ESCC. PMID: 22537917
STC1 is a potentially useful blood marker for predicting biological tumor aggressiveness in patients with gastric cancer PMID: 22889960
STC1 activates a novel anti-oxidant pathway in cardiac myocytes through induction of UCP3 PMID: 22693564
Stanniocalcin (STC1) plays an important role in functional adaption in pediatric kidney transplantation. PMID: 22588538
Overexpression of STC1 was an independent prognostic factor in patients with esophageal cancer who had undergone curative surgery. PMID: 22200953
Level of circulating STC1 mRNA in NSCLC was significantly higher than in benign pulmonary disease or healthy volunteers. Higher levels of circulating STC1 mRNA were associated with more advanced tumor stages and histological subtypes. PMID: 21656524
The results demonstrate that PKCalpha suppresses the expression of STC1 in breast cancer cells. PMID: 21720730
STC-1 could promote angiogenesis in vitro and in vivo, and the angiogenesis was consistent with VEGF expression in vitro. Inhibition of VEGF expression in supernatants with neutralizing antibody markedly abolished angiogenesis induced by STC-1 in vitro. PMID: 21672207
Vascular endothelial growth factor-D stimulates endothelial cell VEGF-A, stanniocalcin-1, and neuropilin-2 and has potent angiogenic effects. PMID: 21474827
STC1 plays an important role in the early response to mechanical injury by epithelial cells by modulating signaling of extracellular ATP PMID: 20422040
STC1 induction by thyroid hormone depends on both TRbeta and PI3K activation. PMID: 20827662
High STC1 gene expression is associated with colorectal cancer. PMID: 21273618
Human stanniocalcin-1 or -2 expressed in mice reduces bone size and severely inhibits cranial intramembranous bone growth. PMID: 20174869

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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